|
|
|
|||||||
|
|||||||||
JBC, Vol. 250, Issue 15, 5995-6001, Aug, 1975
J. P. Bouche, K. Zechel and A. Kornberg
The protein responsible for the initiation of conversion of single-stranded
phage G4 DNA to the duplex replicative form has been purified approximately
3000-fold and identified with Escherichia coli dnaG gene product. The
protein is a rifampicin-resistant RNA polymerase of approximately 64,000
daltons. It catalyzes the incorporation of the four ribonucleoside
triphosphates into an oligoribonucleotide, using as template the
single-stranded DNA coated with the DNA unwinding protein of E. coli. An
RNA transcript of a unique region of the chromosome can serve as a primer
by covalent extension by DNA polymerase III holoenzyme to form a nearly
full-length linear complementary strand. A similar role for the dnaG
protein in the initiation of nascent (Okazaki) fragments in replication of
the host chromosome is discussed.
dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. E. Corn and J. M. Berger Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions Nucleic Acids Res., September 10, 2006; 34(15): 4082 - 4088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Oakley, K. V. Loscha, P. M. Schaeffer, E. Liepinsh, G. Pintacuda, M. C. J. Wilce, G. Otting, and N. E. Dixon Crystal and Solution Structures of the Helicase-binding Domain of Escherichia coli Primase: o J. Biol. Chem., March 25, 2005; 280(12): 11495 - 11504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Mitkova, S. M. Khopde, and S. B. Biswas Mechanism and Stoichiometry of Interaction of DnaG Primase with DnaB Helicase of Escherichia coli in RNA Primer Synthesis J. Biol. Chem., December 26, 2003; 278(52): 52253 - 52261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu and K. J. Marians Purification and Characterization of DnaC810, a Primosomal Protein Capable of Bypassing PriA Function J. Biol. Chem., March 10, 2000; 275(11): 8196 - 8205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Sandler and K. J. Marians Role of PriA in Replication Fork Reactivation in Escherichia coli J. Bacteriol., January 1, 2000; 182(1): 9 - 13. [Full Text]
|
|
|
|
|
|
H. Hiasa and K. J. Marians Initiation of Bidirectional Replication at the Chromosomal Origin Is Directed by the Interaction between Helicase and Primase J. Biol. Chem., September 17, 1999; 274(38): 27244 - 27248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nurse, J. Liu, and K. J. Marians Two Modes of PriA Binding to DNA J. Biol. Chem., August 27, 1999; 274(35): 25026 - 25032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu and K. J. Marians PriA-directed Assembly of a Primosome on D Loop DNA J. Biol. Chem., August 27, 1999; 274(35): 25033 - 25041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, L. Xu, S. J. Sandler, and K. J. Marians Replication fork assembly at recombination intermediates is required for bacterial growth PNAS, March 30, 1999; 96(7): 3552 - 3555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Marians, H. Hiasa, D. R. Kim, and C. S. McHenry Role of the Core DNA Polymerase III Subunits at the Replication Fork. alpha IS THE ONLY SUBUNIT REQUIRED FOR PROCESSIVE REPLICATION J. Biol. Chem., January 23, 1998; 273(4): 2452 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Klann, A. E. Belanger, A. A.-D. Mello, J. Y. Lee, and G. F. Hatfull Characterization of the dnaG Locus in Mycobacterium smegmatis Reveals Linkage of DNA Replication and Cell Division J. Bacteriol., January 1, 1998; 180(1): 65 - 72. [Abstract] [Full Text]
|
|
|
|
|
|
K. Tougu and K. J. Marians The Interaction between Helicase and Primase Sets the Replication Fork Clock J. Biol. Chem., August 30, 1996; 271(35): 21398 - 21405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, P. Nurse, and K. J. Marians The Ordered Assembly of the phi X174-type Primosome. III. PriB FACILITATES COMPLEX FORMATION BETWEEN PriA AND DnaT J. Biol. Chem., June 28, 1996; 271(26): 15656 - 15661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Mustaev and G. N. Godson Studies of the Functional Topography of the Catalytic Center of Escherichia coli Primase J. Biol. Chem., June 30, 1995; 270(26): 15711 - 15718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.M. Stayton, L. Bertsch, S. Biswas, P. Burgers, N. Dixon, J.E. Flynn Jr., R. Fuller, J. Kaguni, J. Kobori, M. Kodaira, et al. Enzymatic Recognition of DNA Replication Origins Cold Spring Harb Symp Quant Biol, January 1, 1983; 47(0): 693 - 700. [Abstract] [PDF]
|
|
|
|
|
|
G. Hobom, R. Grosschedl, M. Lusky, G. Scherer, E. Schwarz, and H. Kossel Functional Analysis of the Replicator Structure of Lambdoid Bacteriophage DNAs Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 165 - 178. [Abstract] [PDF]
|
|
|
|
|
|
K. R. Thomas, P. Manlapaz-Ramos, R. Lundquist, and B. M. Olivera Formation of Okazaki Pieces at the Escherichia coli Replication Fork In Vitro Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 231 - 237. [Abstract] [PDF]
|
|
|
|
|
|
P. Reichard and R. Eliasson Synthesis and Function of Polyoma Initiator RNA Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 271 - 277. [Abstract] [PDF]
|
|
|
|
|
|
R. R. Meyer, J. Shlomai, J. Kobori, D. L. Bates, L. Rowen, R. McMacken, K. Ueda, and A. Kornberg Enzymatic Conversion of Single-stranded {phi}X174 and G4 Circles to Duplex Forms: Discontinuous Replication Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 289 - 293. [Abstract] [PDF]
|
|
|
|
|
|
S. H. Wickner DNA Replication Proteins of Escherichia coli and Phage {lambda} Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 303 - 310. [Abstract] [PDF]
|
|
|
|
|
|
C. Sumida-Yasumoto, J.-E. Ikeda, E. Benz, K. J. Marians, R. Vicuna, S. Sugrue, S. L. Zipursky, and J. Hurwitz Replication of {phi}X174 DNA: In Vitro Synthesis of {phi}X RFI DNA and Circular, Single-stranded DNA Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 311 - 329. [Abstract] [PDF]
|
|
|
|
|
|
J. Sims, K. Koths, and D. Dressler Single-stranded Phage Replication: Positive- and Negative-strand DNA Synthesis Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 349 - 365. [Abstract] [PDF]
|
|
|
|
|
|
G. N. Godson G4 DNA Replication In Vivo and the Origins of Its Strand Synthesis Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 367 - 377. [Abstract] [PDF]
|
|
|
|
|
|
G. Hillenbrand, G. Morelli, E. Lanka, and E. Scherzinger Bacteriophage T7 DNA Primase: A Multifunctional Enzyme Involved in DNA Replication Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 449 - 459. [Abstract] [PDF]
|
|
|
|
 |
|
 I. Lehman and D. Uyemura DNA polymerase I: essential replication enzyme Science, September 10, 1976; 193(4257): 963 - 969. [PDF]
|
|
|
|
|
|
P. Chang and K. J. Marians Identification of a Region of Escherichia coli DnaB Required for Functional Interaction with DnaG at the Replication Fork J. Biol. Chem., August 18, 2000; 275(34): 26187 - 26195. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |