JBC, Vol. 250, Issue 18, 7188-7193, Sep, 1975
Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. I. Quantitative covariance of the rate of glucose utilization and the cellular level of fructose 1,6-diphosphate during exponential growth and nutrient limitation
D. N. Dietzler, M. P. Leckie, P. E. Bergstein and M. J. Sughrue
In cultures of Escherichia coli W4597(K) and G34 under various nutritional
conditions the rates of glucose utilization and cellular levels of
fructose-1,6-P2 are quantitatively related by the Hill equation where the
value of the Hill coefficient is approximately equal to 2. This is the
first evidence that fructose-P2, or any metabolite which covaries with
fructose-P2, modulates glucose utilization in E. coli. In light of previous
observations from our laboratory this new observation and those in the
succeeding report provide the first evidence that in E. coli glycolysis,
glycogen synthesis and glucose utilization are coordinately regulated, thus
providing for the coupling of ATP utilization and production under various
metabolic circumstances. Alterations in the level of ATP apparently affect
the velocity of phosphofructokinase, the rate-limiting enzyme in
glycolysis, altering the cellular levels of glucose-6-P or fructose-P2.
Changes in the levels of these hexose phosphates are quantitatively related
to alterations in the rates of glucose utilization and glycogen synthesis
in the intact E. coli cell.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?