HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Meijer, A. J. Articles by Williamson, J. R. Search for Related Content PubMed PubMed Citation Articles by Meijer, A. J. Articles by Williamson, J. R. Social Bookmarking                      What's this?

JBC, Vol. 250, Issue 19, 7728-7738, Oct, 1975

Role of anion translocation across the mitochondrial membrane in the regulation of urea synthesis from ammonia by isolated rat hepatocytes

A. J. Meijer, J. A. Gimpel, G. A. Deleeuw, J. M. Tager and J. R. Williamson

The regulation of urea synthesis from ammonia was investigated using isolated hepatocytes from fasted rats. Addition of ammonia alone produced only a small increase of urea formation, which was stimulated 2-fold by ornithine in conjunction with a fall of ATP levels and an accumulation of citrulline. Further addition of oleate or beta-hydroxybutyrate produced an additional 2-fold stimulation of urea formation to approximately 200 mumol/g dry weight/hour. The presence of oleate also protected against the inhibitory effect of 2,4-dinitrophenol on urea synthesis and the cellular ATP content. The data suggest that both the rate of of energy production and the rate of generation of reducing equivalents from endogensou substrates are insufficient to meet the requirements for optimal rates of urea synthesis. Urea formation from NH3 in the presence of ornithine and oleate, but iin the absence of gluconeogenic precursors, was inhibited by butylmalonate, a known inhibitor of malate-phosphate exchange across the mitochondrial membrane, and stimulated by theaddition of malate and other dicarboxylic acids and amino acids to the cell suspension...
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Fiermonte, V. Dolce, L. David, F. M. Santorelli, C. Dionisi-Vici, F. Palmieri, and J. E. Walker The Mitochondrial Ornithine Transporter: BACTERIAL EXPRESSION, RECONSTITUTION, FUNCTIONAL CHARACTERIZATION, AND TISSUE DISTRIBUTION OF TWO HUMAN ISOFORMS J. Biol. Chem., August 29, 2003; 278(35): 32778 - 32783. [Abstract] [Full Text] [PDF]

				S. v. Dahl, F. Schliess, R. Reissmann, B. Gorg, O. Weiergraber, M. Kocalkova, F. Dombrowski, and D. Haussinger Involvement of Integrins in Osmosensing and Signaling toward Autophagic Proteolysis in Rat Liver J. Biol. Chem., July 11, 2003; 278(29): 27088 - 27095. [Abstract] [Full Text] [PDF]

				J. G. Bode, T. Peters–Regehr, R. Kubitz, and D. Häussinger Expression of Glutamine Synthetase in Macrophages J. Histochem. Cytochem., March 1, 2000; 48(3): 415 - 422. [Abstract] [Full Text]

				M. Wettstein, T. Peters-Regehr, R. Kubitz, R. Fischer, C. Holneicher, I. Monnighoff, and D. Haussinger Release of osmolytes induced by phagocytosis and hormones in rat liver Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G227 - G233. [Abstract] [Full Text] [PDF]