HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shulman, R. W. Articles by Warner, J. R. Search for Related Content PubMed PubMed Citation Articles by Shulman, R. W. Articles by Warner, J. R. Social Bookmarking                      What's this?

JBC, Vol. 252, Issue 4, 1344-1349, Feb, 1977

Noncoordinated transcription in the absence of protein synthesis in yeast

R. W. Shulman, C. E. Sripati and J. R. Warner

In the yeast Saccharomyces cerevisiae we have carried out a detailed study of the response of transcription to the inhibition of translation. Measurement of the incorporation of labeled bases and nucleosides into the nucleoside triphosphate pools revealed that amino acid deprivation brings about a 10- to 50-fold inhibition of such labeling. Therefore accurate comparisons of RNA synthesis using such precursors are difficult to obtain. To overcome this problem we have turned to the use of L-[methyl-3H] methionine as a precursor, because the labeling of the S-adenosylmethionine pool is relatively unaffected by the rate of protein synthesis. Using this precursor, we have observed that in the absence of protein synthesis the transcription of ribosomal RNA is reduced by 80%, the transcription of messenger RNA is reduced by about 25%, and the transcription of transfer RNA is reduced by less than 20%. These results are obtained when protein synthesis is inhibited either by deprivation of an amino acid or by the addition of cycloheximide. Ribosomal precursor RNA synthesized in the absence of protein synthesis is fully methylated. We conclude that the transcription of rRNA is the primary target of stringent control. Furthermore the inhibition of protein synthesis, itself, may be the trigger for this response.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Conesa, R. Ruotolo, P. Soularue, T. A. Simms, D. Donze, A. Sentenac, and G. Dieci Modulation of Yeast Genome Expression in Response to Defective RNA Polymerase III-Dependent Transcription Mol. Cell. Biol., October 1, 2005; 25(19): 8631 - 8642. [Abstract] [Full Text] [PDF]

				K. Pluta, O. Lefebvre, N. C. Martin, W. J. Smagowicz, D. R. Stanford, S. R. Ellis, A. K. Hopper, A. Sentenac, and M. Boguta Maf1p, a Negative Effector of RNA Polymerase III in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 2001; 21(15): 5031 - 5040. [Abstract] [Full Text] [PDF]

				M. Nomura Regulation of Ribosome Biosynthesis in Escherichia coli and Saccharomyces cerevisiae: Diversity and Common Principles J. Bacteriol., November 15, 1999; 181(22): 6857 - 6864. [Full Text]

				E. M. Clarke, C. L. Peterson, A. V. Brainard, and D. L. Riggs Regulation of the RNA Polymerase I and III Transcription Systems in Response to Growth Conditions J. Biol. Chem., September 6, 1996; 271(36): 22189 - 22195. [Abstract] [Full Text] [PDF]

				G. Dieci, L. Duimio, G. Peracchia, and S. Ottonello Selective Inactivation of Two Components of the Multiprotein Transcription Factor TFIIIB in Cycloheximide Growth-arrested Yeast Cells J. Biol. Chem., June 2, 1995; 270(22): 13476 - 13482. [Abstract] [Full Text] [PDF]

				D. R. W. Edwards, J. B. Taylor, W. F. Wakeling, F. Z. Watts, and I. R. Johnston Studies on the Prereplicative Phase of the Cell Cycle in Saccharomyces cerevisiae Cold Spring Harb Symp Quant Biol, January 1, 1979; 43(0): 577 - 586. [Abstract] [PDF]