HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heinegard, D. Search for Related Content PubMed PubMed Citation Articles by Heinegard, D. Social Bookmarking                      What's this?

JBC, Vol. 252, Issue 6, 1980-1989, Mar, 1977

Polydispersity of cartilage proteoglycans. Structural variations with size and buoyant density of the molecules

D. Heinegard

Proteoglycan monomers were subfractionated according to buoyant density by dissociative CsCl density gradient centrifugation. It was shown that with decreasing buoyant density of the proteoglycan subfraction: (a) the average sizes of the molecules decreased (shown by Sepharose 2B chromatography); (b) the relative content of chondroitin sulfate decreased; (c) the relative content of protein increased; (d) the relative proportion of the amino acids glycine and serine, which occur close to the chondroitin sulfate-peptide linkage, decreased; (e) the relative proportion of the hyaluronic acid-binding region released by treatment of proteoglycans with cyanogen bromide increased; (f) the relative content of the keratan sulfate-enriched region increased. The data indicate that proteoglycans contain a nonvariable hyaluronic acid-binding region, a keratan sulfate-enriched region and a chondroitin sulfate-enriched region of variable size. It is concluded that proteoglycans vary in size mainly because of variations in the size of the chondroitin sulfate-enriched region. Additional data were obtained using subfractions of proteoglycan monomers isolated according to size differences by using Sepharose 2B chromatography. The Kav values of the subfractions on Sepharose 2B ranged from 0 to 0.54. Analyses of these subfractions showed the same variations with size of the content of chondroitin sulfate, protein, amino acids, hyaluronic acid-binding region, and keratan sulfate-enriched region, as was shown for the subfractions isolated at different buoyant densities in the dissociative gradient.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Pothacharoen, K. Kalayanamitra, S. S. Deepa, S. Fukui, T. Hattori, N. Fukushima, T. Hardingham, P. Kongtawelert, and K. Sugahara Two Related but Distinct Chondroitin Sulfate Mimetope Octasaccharide Sequences Recognized by Monoclonal Antibody WF6 J. Biol. Chem., November 30, 2007; 282(48): 35232 - 35246. [Abstract] [Full Text] [PDF]

				P. Pothacharoen, S. Siriaunkgul, S. Ong-Chai, J. Supabandhu, P. Kumja, C. Wanaphirak, K. Sugahara, T. Hardingham, and P. Kongtawelert Raised Serum Chondroitin Sulfate Epitope Level in Ovarian Epithelial Cancer J. Biochem., October 1, 2006; 140(4): 517 - 524. [Abstract] [Full Text] [PDF]

				S. S. Sivan, E. Tsitron, E. Wachtel, P. J. Roughley, N. Sakkee, F. van der Ham, J. DeGroot, S. Roberts, and A. Maroudas Aggrecan Turnover in Human Intervertebral Disc as Determined by the Racemization of Aspartic Acid J. Biol. Chem., May 12, 2006; 281(19): 13009 - 13014. [Abstract] [Full Text] [PDF]

				S. Shi, S. Grothe, Y. Zhang, M. D. O'Connor-McCourt, A. R. Poole, P. J. Roughley, and J. S. Mort Link Protein Has Greater Affinity for Versican than Aggrecan J. Biol. Chem., March 26, 2004; 279(13): 12060 - 12066. [Abstract] [Full Text] [PDF]

				M. Wendel, Y. Sommarin, and D. Heinegard Bone Matrix Proteins: Isolation and Characterization of a Novel Cell-binding Keratan Sulfate Proteoglycan (Osteoadherin) from Bovine Bone J. Cell Biol., May 4, 1998; 141(3): 839 - 847. [Abstract] [Full Text] [PDF]

				N. Hauser, M. Paulsson, D. Heinegard, and M. Morgelin Interaction of Cartilage Matrix Protein with Aggrecan. INCREASED COVALENT CROSS-LINKING WITH TISSUE MATURATION J. Biol. Chem., December 13, 1996; 271(50): 32247 - 32252. [Abstract] [Full Text] [PDF]

				A. Caplan, M. Fiszman, and H. Eppenberger Molecular and cell isoforms during development Science, September 2, 1983; 221(4614): 921 - 927. [Abstract] [PDF]