HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alexander, M. Articles by Goodman, H. M. Search for Related Content PubMed PubMed Citation Articles by Alexander, M. Articles by Goodman, H. M. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 22, 11978-11985, Oct, 1985

Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase

M Alexander, G Curtis, J Avruch and HM Goodman

The effect of insulin on protein biosynthesis was examined in differentiated 3T3-L1 and 3T3-F442A adipocytes. Insulin altered the relative rate of synthesis of specific proteins independent of its ability to hasten conversion of the fibroblast (preadipocyte) phenotype to the adipocyte phenotype. Although more than one pattern of response to insulin was observed, we focused on the induction of a Mr 33,000 protein which was identified as the glycolytic enzyme glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Exposure of 3T3 adipocytes to insulin throughout differentiation specifically increased GAPDH activity and protein content by 2- to 3-fold as compared to 3T3 adipocytes differentiated in the absence of insulin. These changes in enzyme activity and content could be accounted for by a 4-fold increase in the relative rate of synthesis of GAPDH and a 9-fold increase in hybridizable mRNA levels. Within 2 h of insulin addition to 3T3 adipocytes differentiated in the absence of hormone, hybridizable GAPDH mRNA levels increased 3-fold, and within 24 h GAPDH mRNA levels increased 8-fold, and [35S] methionine incorporation into GAPDH protein increased 5-fold. The increase in GAPDH mRNA and GAPDH biosynthesis could be demonstrated using physiologic concentrations of insulin (0.24 nM), indicating that these effects are mediated through a specific interaction with the insulin receptor. These studies demonstrate that insulin, as the sole hormonal perturbant, can increase the synthesis of certain 3T3 adipocyte proteins by altering the cellular content of a specific mRNA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. K Berg, C. Li, G. Asher, D. N Wells, and B. Oback Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny Biol Reprod, September 1, 2007; 77(3): 384 - 394. [Abstract] [Full Text] [PDF]

				S. L. Lay, I. Lefrere, C. Trautwein, I. Dugail, and S. Krief Insulin and Sterol-regulatory Element-binding Protein-1c (SREBP-1C) Regulation of Gene Expression in 3T3-L1 Adipocytes. IDENTIFICATION OF CCAAT/ENHANCER-BINDING PROTEIN beta AS AN SREBP-1C TARGET J. Biol. Chem., September 13, 2002; 277(38): 35625 - 35634. [Abstract] [Full Text] [PDF]

				M. J. Stevens, Y. Hosaka, J. A. Masterson, S. M. Jones, T. P. Thomas, and D. D. Larkin Downregulation of the human taurine transporter by glucose in cultured retinal pigment epithelial cells Am J Physiol Endocrinol Metab, October 1, 1999; 277(4): E760 - E771. [Abstract] [Full Text] [PDF]

				A. I. Loaiza-Pérez, M.-T. Seisdedos, D. L. Kleiman de Pisarev, H. A. Sancovich, A. S. Randi, A. M. Ferramola de Sancovich, and P. Santisteban Hexachlorobenzene, a Dioxin-Type Compound, Increases Malic Enzyme Gene Transcription through a Mechanism Involving the Thyroid Hormone Response Element Endocrinology, September 1, 1999; 140(9): 4142 - 4151. [Abstract] [Full Text]

				F Leyva, T-P Chua, I F Godsland, A J S Coats, and S D Anker Loss of the normal coupling between the anaerobic threshold and insulin sensitivity in chronic heart failure Heart, September 1, 1999; 82(3): 348 - 351. [Abstract] [Full Text] [PDF]

				K. Ranganna and F. M. Yatsu Inhibition of Platelet-Derived Growth Factor BB–Induced Expression of Glyceraldehyde- 3-Phosphate Dehydrogenase by Sodium Butyrate in Rat Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3420 - 3427. [Abstract] [Full Text]

				E. Hu, P. Liang, and B. M. Spiegelman AdipoQ Is a Novel Adipose-specific Gene Dysregulated in Obesity J. Biol. Chem., May 3, 1996; 271(18): 10697 - 10703. [Abstract] [Full Text] [PDF]

				R. O'Brien, P. Lucas, C. Forest, M. Magnuson, and D. Granner Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription Science, August 3, 1990; 249(4968): 533 - 537. [Abstract] [PDF]