HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hohengasser, C. J. Articles by Wirtz, K. W. Search for Related Content PubMed PubMed Citation Articles by Hohengasser, C. J. Articles by Wirtz, K. W. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 14, 6255-6259, May, 1986

Phosphorylation and degradation of exogenous phosphatidylinositol incorporated into Friend erythroleukemic cells

CJ Hohengasser, JT Thornburg, PA van Paridon, P van der Schaft and KW Wirtz

Phosphatidyl[2-3H]inositol (PtdIns) obtained from rat skeletal muscle and yeast was introduced into Friend erythroleukemic cells by use of the PtdInstransfer protein or by spontaneous route. The mammalian PtdIns incorporated by the transfer protein appeared metabolically inert while the spontaneously incorporated PtdIns was both phosphorylated to PtdIns-4-phosphate (i.e. 30% of the total PtdIns incorporated) and converted into lyso-PtdIns (i.e. 20% of the total PtdIns incorporated); formation of PtdIns, 4,5-bisphosphate was minimal. The extensive metabolism of the spontaneously incorporated PtdIns strongly suggests that this PtdIns does not rapidly equilibrate with the endogenous PtdIns pools. A similar spontaneous incorporation of yeast PtdIns was accompanied by a negligible degree of phosphorylation and hydrolysis. Evidence is provided that this difference in metabolism reflects the absence of arachidonate in the yeast PtdIns.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P.-y. Wang and R. S. Munford CD14-dependent Internalization and Metabolism of Extracellular Phosphatidylinositol by Monocytes J. Biol. Chem., August 13, 1999; 274(33): 23235 - 23241. [Abstract] [Full Text] [PDF]

				A. Wongkajornsilp and T. L. Rosenberry Uptake of Exogenous sn-1-Acyl-2- lyso-phosphatidylinositol into HeLa S3 Cells J. Biol. Chem., April 21, 1995; 270(16): 9147 - 9153. [Abstract] [Full Text] [PDF]