HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cole, K. D. Articles by Kistler, W. S. Search for Related Content PubMed PubMed Citation Articles by Cole, K. D. Articles by Kistler, W. S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 16, 7178-7183, 06, 1986

Isolation of the gene for the testis-specific H1 histone variant H1t

KD Cole, JC Kandala and WS Kistler

H1t is a testis-specific H1 variant found in pachytene spermatocytes and round spermatids of mammals. The H1t gene was isolated from the Sargent-Bonner library of recombinant lambda bacteriophage containing EcoRI fragments of rat liver DNA using a hybridization probe derived from a chicken H1 variant. The rat H1t gene encodes a 207-amino acid protein (ignoring the initiating methionine) that matches perfectly what is known of the sequence and composition of H1t isolated from rat testes. The gene lacks introns and has good matches to all the consensus sequences known to lie upstream from a variety of H1 genes from diverse organisms. It also has the standard downstream palindromic sequence that specifies the 3'-end of most histone messages. Accordingly, the features of the gene or its environs that restrict its expression to a particular phase of spermatogenesis are not yet evident.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. C. Horvath, W. S. Kistler, and M. K. Kistler RFX2 Is a Potential Transcriptional Regulatory Factor for Histone H1t and Other Genes Expressed During the Meiotic Phase of Spermatogenesis Biol Reprod, November 1, 2004; 71(5): 1551 - 1559. [Abstract] [Full Text] [PDF]

				W. Yan, L. Ma, K. H. Burns, and M. M. Matzuk HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis PNAS, September 2, 2003; 100(18): 10546 - 10551. [Abstract] [Full Text] [PDF]

				S. A. Wolfe and S. R. Grimes Specific Binding of Nuclear Proteins to a Bifunctional Promoter Element Upstream of the H1/AC Box of the Testis-Specific Histone H1t Gene Biol Reprod, June 1, 2003; 68(6): 2267 - 2273. [Abstract] [Full Text] [PDF]

				G. C. Horvath, S. E. Clare, M. K. Kistler, and W. Stephen Kistler Characterization of the H1t Promoter: Role of Conserved Histone 1 AC and TG Elements and Dominance of the Cap-Proximal Silencer Biol Reprod, October 1, 2001; 65(4): 1074 - 1081. [Abstract] [Full Text] [PDF]

				D. A. Fantz, W. R. Hatfield, G. Horvath, M. K. Kistler, and W. S. Kistler Mice with a Targeted Disruption of the H1t Gene Are Fertile and Undergo Normal Changes in Structural Chromosomal Proteins During Spermiogenesis Biol Reprod, February 1, 2001; 64(2): 425 - 431. [Abstract] [Full Text]

				J. G. Bartell, D. A. Fantz, T. Davis, M. J. Dewey, M. K. Kistler, and W. S. Kistler Elimination of Male Germ Cells in Transgenic Mice by the Diphtheria Toxin A Chain Gene Directed by the Histone H1t Promoter Biol Reprod, August 1, 2000; 63(2): 409 - 416. [Abstract] [Full Text]

				Q. Lin, A. Sirotkin, and A. I. Skoultchi Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t Mol. Cell. Biol., March 15, 2000; 20(6): 2122 - 2128. [Abstract] [Full Text]

				L. Cataldo, M.-A. Mastrangelo, and K. C. Kleene A quantitative sucrose gradient analysis of the translational activity of 18 mRNA species in testes from adult mice Mol. Hum. Reprod., March 1, 1999; 5(3): 206 - 213. [Abstract] [Full Text] [PDF]

				J. M. vanWert, H. R. Panek, S. A. Wolfe, and S. R. Grimes The TE Promoter Element of the Histone H1t Gene Is Essential for Transcription in Transgenic Mouse Primary Spermatocytes Biol Reprod, July 1, 1998; 59(3): 704 - 710. [Abstract] [Full Text]

				S. E. Clare, D. A. Fantz, W. S. Kistler, and M. K. Kistler The Testis-specific Histone H1t Gene Is Strongly Repressed by a G/C-rich Region Just Downstream of the TATA Box J. Biol. Chem., December 26, 1997; 272(52): 33028 - 33036. [Abstract] [Full Text] [PDF]

				J. G. Bartell, T. Davis, E. J. Kremer, M. J. Dewey, and W. S. Kistler Expression of the Rat Testis-specific Histone H1t Gene in Transgenic Mice J. Biol. Chem., February 23, 1996; 271(8): 4046 - 4054. [Abstract] [Full Text] [PDF]

				R C Smith, E Dworkin-Rastl, and M B Dworkin Expression of a histone H1-like protein is restricted to early Xenopus development. Genes & Dev., October 1, 1988; 2(10): 1284 - 1295. [Abstract] [PDF]