HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rujanavech, C. Articles by Silbert, D. F. Search for Related Content PubMed PubMed Citation Articles by Rujanavech, C. Articles by Silbert, D. F. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 16, 7196-7203, Jun, 1986

LM cell growth and membrane lipid adaptation to sterol structure

C Rujanavech and DF Silbert

Using a sterol auxotroph of the LM cell mouse fibroblast, we demonstrate that relatively few cholesterol analogues can substitute for cholesterol as a growth factor. The auxotroph grows normally on desmosterol and trans-22-dehydrocholesterol and at reduced rates on dihydrocholesterol, campesterol, and 22,23-dihydrobrassicasterol. It does not grow with beta-sitosterol, stigmasterol, ergosterol, or cis-22- dehydrocholesterol when the sterol is present as sole supplement but does grow at normal rates when the analogue is supplied with suboptimal amounts of cholesterol. Two contrasting types of membrane lipid changes are observed in cells grown on cholesterol analogues. In cells grown with dihydrocholesterol, a marked increase in desaturation and elongation of fatty acids is noted. Conversely, when cells are grown with cis-22-dehydrocholesterol, desaturation and elongation of fatty acids are severely curtailed. Cells grown on alkyl sterols respond like cells grown on cis-22-dehydrocholesterol but in a less pronounced fashion. The effects of sterol substitution in mammalian cells versus in lower eukaryotes are compared, and an explanation for the secondary changes in fatty acid composition in terms of phospholipid phase behavior is suggested.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Xu, S. D. Rychnovsky, J. D. Belani, H. H. Hobbs, J. C. Cohen, and R. B. Rawson Dual roles for cholesterol in mammalian cells PNAS, October 11, 2005; 102(41): 14551 - 14556. [Abstract] [Full Text] [PDF]

				C. Fernandez, M. Martin, D. Gomez-Coronado, and M. A. Lasuncion Effects of distal cholesterol biosynthesis inhibitors on cell proliferation and cell cycle progression J. Lipid Res., May 1, 2005; 46(5): 920 - 929. [Abstract] [Full Text] [PDF]

				I. Nilsson, H. Ohvo-Rekila, J. P. Slotte, A. E. Johnson, and G. von Heijne Inhibition of Protein Translocation across the Endoplasmic Reticulum Membrane by Sterols J. Biol. Chem., November 2, 2001; 276(45): 41748 - 41754. [Abstract] [Full Text] [PDF]

				C. Roux, C. Wolf, N. Mulliez, W. Gaoua, V. Cormier, F. Chevy, and D. Citadelle Role of cholesterol in embryonic development Am. J. Clinical Nutrition, May 1, 2000; 71(5): 1270S - 1279. [Abstract] [Full Text] [PDF]

				J. MARTÍNEZ-BOTAS, Y. SUÁREZ, A. J. FERRUELO, D. GÓMEZ-CORONADO, and M. A. LASUNCIÓN Cholesterol starvation decreases P34cdc2 kinase activity and arrests the cell cycle at G2 FASEB J, August 1, 1999; 13(11): 1359 - 1370. [Abstract] [Full Text]

				S. J. Fliesler, M. J. Richards, C.-y. Miller, and N. S. Peachey Marked Alteration of Sterol Metabolism and Composition without Compromising Retinal Development or Function Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1792 - 1801. [Abstract] [Full Text] [PDF]