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J. Biol. Chem., Vol. 261, Issue 18, 8122-8127, 06, 1986

Cellular transglutaminase. Lung matrix-associated transglutaminase: characterization and activation with sulfhydryls

ET Cocuzzi and SI Chung

Transglutaminase in the rat lung is tightly associated with the insoluble matrix which is not extractable with detergent, 0.5 M NaCl, and 40% glycerol solutions. The insoluble matrix was found to be rich in heparin sulfate and poor in collagen, elastin, and DNA. The lung transglutaminase was found to be distinct from tissue transglutaminase (identifiable with the well-characterized guinea pig liver transglutaminase) in its retention volume in DEAE-Sephacel columns and its Kd value in gel-filtration columns. The enzyme was activated 6-8- fold with the sulfhydryl reagent dithiothreitol. This activation was accompanied with the dissociation of enzyme from the tightly bound insoluble matrix and resulted in changes of the molecular properties of the enzyme--increase in affinity for anion-exchanger and decrease in Stokes radius. Addition of 50 mM KSCN induced a 2-fold increase in SH- dependent activation of transglutaminase activity. These results suggest that sulfhydryl agents may play a role in the activation and compartmental translocation of the transglutaminase in the lung.
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