HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baudier, J. Articles by Gerard, D. Search for Related Content PubMed PubMed Citation Articles by Baudier, J. Articles by Gerard, D. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 18, 8192-8203, Jun, 1986

Ions binding to S100 proteins. I. Calcium- and zinc-binding properties of bovine brain S100 alpha alpha, S100a (alpha beta), and S100b (beta beta) protein: Zn2+ regulates Ca2+ binding on S100b protein

J Baudier, N Glasser and D Gerard

Flow dialysis measurements of calcium binding to bovine brain S100 alpha alpha, S100a (alpha beta), and S100b (beta beta) proteins in 20 mM Tris-HCl buffer at pH 7.5 and 8.3 revealed that S100 proteins bind specifically 4 Ca2+ eq/mol of protein dimer. The specific calcium- binding sites had, therefore, been assigned to typical amino acid sequences on the alpha and beta subunit. The protein affinity for calcium is much lower in the presence of magnesium and potassium. Potassium strongly antagonizes calcium binding on two calcium-binding sites responsible for most of the Ca2+-induced conformational changes on S100 proteins (probably site II alpha and site II beta). Zinc- binding studies in the absence of divalent cations revealed eight zinc- binding sites/mol of S100b protein dimer that we assumed to correspond to 4 zinc-binding sites/beta subunit. Zinc binding to S100b studied with UV spectroscopy methods showed that the occupation of the four higher affinity sites and the four lower affinity sites on the protein dimer were responsible for different conformational changes in S100b structure. Zinc binding on the higher affinity sites regulates calcium binding to S100b by increasing the protein affinity for calcium and decreasing the antagonistic effect of potassium on calcium binding. Zinc-binding studies on S100a and S100 alpha alpha protein showed that the Trp-containing S100 proteins bind zinc more weakly than S100b protein. Calcium-binding studies on zinc-bound S100a proved that calcium- and zinc-binding sites were distinct although there was no increase in zinc-bound S100a affinity for calcium, as in S100b protein. Finally we provide evidence that discrepancies between previously published results on the optical properties of S100b protein probably result from oxidation of the sulfhydryl groups in the protein.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Rosenberger, I. S. Thorey, S. Werner, and P. Boukamp A Novel Regulator of Telomerase: S100A8 MEDIATES DIFFERENTIATION-DEPENDENT AND CALCIUM-INDUCED INHIBITION OF TELOMERASE ACTIVITY IN THE HUMAN EPIDERMAL KERATINOCYTE LINE HaCaT J. Biol. Chem., March 2, 2007; 282(9): 6126 - 6135. [Abstract] [Full Text] [PDF]

				E. J. Kim and D. M. Helfman Characterization of the Metastasis-associated Protein, S100A4: ROLES OF CALCIUM BINDING AND DIMERIZATION IN CELLULAR LOCALIZATION AND INTERACTION WITH MYOSIN J. Biol. Chem., August 8, 2003; 278(32): 30063 - 30073. [Abstract] [Full Text] [PDF]

				P. Most, A. Remppis, C. Weber, J. Bernotat, P. Ehlermann, S. T. Pleger, W. Kirsch, M. Weber, D. Uttenweiler, G. L. Smith, et al. The C Terminus (Amino Acids 75-94) and the Linker Region (Amino Acids 42-54) of the Ca2+-binding Protein S100A1 Differentially Enhance Sarcoplasmic Ca2+ Release in Murine Skinned Skeletal Muscle Fibers J. Biol. Chem., July 11, 2003; 278(29): 26356 - 26364. [Abstract] [Full Text] [PDF]

				J. A. R. Hobbs, R. May, K. Tanousis, E. McNeill, M. Mathies, C. Gebhardt, R. Henderson, M. J. Robinson, and N. Hogg Myeloid Cell Function in MRP-14 (S100A9) Null Mice Mol. Cell. Biol., April 1, 2003; 23(7): 2564 - 2576. [Abstract] [Full Text] [PDF]

				G. O. Mbele, J. C. Deloulme, B. J. Gentil, C. Delphin, M. Ferro, J. Garin, M. Takahashi, and J. Baudier The Zinc- and Calcium-binding S100B Interacts and Co-localizes with IQGAP1 during Dynamic Rearrangement of Cell Membranes J. Biol. Chem., December 13, 2002; 277(51): 49998 - 50007. [Abstract] [Full Text] [PDF]

				P. Most, J. Bernotat, P. Ehlermann, S. T. Pleger, M. Reppel, M. Borries, F. Niroomand, B. Pieske, P. M. L. Janssen, T. Eschenhagen, et al. S100A1: A regulator of myocardial contractility PNAS, November 20, 2001; 98(24): 13889 - 13894. [Abstract] [Full Text] [PDF]

				T. B. Stradal, H. Troxler, C. W. Heizmann, and M. Gimona Mapping the Zinc Ligands of S100A2 by Site-directed Mutagenesis J. Biol. Chem., April 28, 2000; 275(18): 13219 - 13227. [Abstract] [Full Text] [PDF]

				K. Ridinger, B. W. Schafer, I. Durussel, J. A. Cox, and C. W. Heizmann S100A13. BIOCHEMICAL CHARACTERIZATION AND SUBCELLULAR LOCALIZATION IN DIFFERENT CELL LINES J. Biol. Chem., March 17, 2000; 275(12): 8686 - 8694. [Abstract] [Full Text] [PDF]

				C. Scotto, C. Delphin, J. C. Deloulme, and J. Baudier Concerted Regulation of Wild-Type p53 Nuclear Accumulation and Activation by S100B and Calcium-Dependent Protein Kinase C Mol. Cell. Biol., October 1, 1999; 19(10): 7168 - 7180. [Abstract] [Full Text] [PDF]

				T. Tanaka, N. Miwa, S. Kawamura, H. Sohma, K. Nitta, and N. Matsushima Molecular modeling of single polypeptide chain of calcium-binding protein p26olf from dimeric S100B(ßß) Protein Eng. Des. Sel., May 1, 1999; 12(5): 395 - 405. [Abstract] [Full Text] [PDF]

				C. Delphin, M. Ronjat, J. C. Deloulme, G. Garin, L. Debussche, Y. Higashimoto, K. Sakaguchi, and J. Baudier Calcium-dependent Interaction of S100B with the C-terminal Domain of the Tumor Suppressor p53 J. Biol. Chem., April 9, 1999; 274(15): 10539 - 10544. [Abstract] [Full Text] [PDF]

				K. R. Barber, K. A. McClintock, G. A. Jamieson Jr., R. V. W. Dimlich, and G. S. Shaw Specificity and Zn2+ Enhancement of the S100B Binding Epitope TRTK-12 J. Biol. Chem., January 15, 1999; 274(3): 1502 - 1508. [Abstract] [Full Text] [PDF]

				C. Franz, I. Durussel, J. A. Cox, B. W. Schafer, and C. W. Heizmann Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins J. Biol. Chem., July 24, 1998; 273(30): 18826 - 18834. [Abstract] [Full Text] [PDF]

				C. Scotto, J. C. Deloulme, D. Rousseau, E. Chambaz, and J. Baudier Calcium and S100B Regulation of p53-Dependent Cell Growth Arrest and Apoptosis Mol. Cell. Biol., July 1, 1998; 18(7): 4272 - 4281. [Abstract] [Full Text]

				C. Scotto, Y. Mely, H. Ohshima, J. Garin, C. Cochet, E. Chambaz, and J. Baudier Cysteine Oxidation in the Mitogenic S100B Protein Leads to Changes in Phosphorylation by Catalytic CKII-alpha Subunit J. Biol. Chem., February 13, 1998; 273(7): 3901 - 3908. [Abstract] [Full Text] [PDF]

				R. A. Newton and N. Hogg The Human S100 Protein MRP-14 Is a Novel Activator of the {beta}2 Integrin Mac-1 on Neutrophils J. Immunol., February 1, 1998; 160(3): 1427 - 1435. [Abstract] [Full Text] [PDF]

				U. Missler, M. Wiesmann, C. Friedrich, and M. Kaps S-100 Protein and Neuron-Specific Enolase Concentrations in Blood as Indicators of Infarction Volume and Prognosis in Acute Ischemic Stroke Stroke, October 1, 1997; 28(10): 1956 - 1960. [Abstract] [Full Text]

				T. Nishikawa, I. S. M. Lee, N. Shiraishi, T. Ishikawa, Y. Ohta, and M. Nishikimi Identification of S100b Protein as Copper-binding Protein and Its Suppression of Copper-induced Cell Damage J. Biol. Chem., September 12, 1997; 272(37): 23037 - 23041. [Abstract] [Full Text] [PDF]

				U. G. Föhr, C. W. Heizmann, D. Engelkamp, B. W. Schäfer, and J. A. Cox Purification and Cation Binding Properties of the Recombinant Human S100 Calcium-binding Protein A3, an EF-hand Motif Protein with High Affinity for Zinc J. Biol. Chem., September 8, 1995; 270(36): 21056 - 21061. [Abstract] [Full Text] [PDF]

				B. W. Schafer, J.-M. Fritschy, P. Murmann, H. Troxler, I. Durussel, C. W. Heizmann, and J. A. Cox Brain S100A5 Is a Novel Calcium-, Zinc-, and Copper Ion-binding Protein of the EF-hand Superfamily J. Biol. Chem., September 22, 2000; 275(39): 30623 - 30630. [Abstract] [Full Text] [PDF]

				J. C. Deloulme, N. Assard, G. O. Mbele, C. Mangin, R. Kuwano, and J. Baudier S100A6 and S100A11 Are Specific Targets of the Calcium- and Zinc-binding S100B Protein in Vivo J. Biol. Chem., November 3, 2000; 275(45): 35302 - 35310. [Abstract] [Full Text] [PDF]

				B. J. Gentil, C. Delphin, G. O. Mbele, J. C. Deloulme, M. Ferro, J. Garin, and J. Baudier The Giant Protein AHNAK Is a Specific Target for the Calcium- and Zinc-binding S100B Protein. POTENTIAL IMPLICATIONS FOR Ca2+ HOMEOSTASIS REGULATION BY S100B J. Biol. Chem., June 22, 2001; 276(26): 23253 - 23261. [Abstract] [Full Text] [PDF]

				G. E. Davey, P. Murmann, and C. W. Heizmann Intracellular Ca2+ and Zn2+ Levels Regulate the Alternative Cell Density-dependent Secretion of S100B in Human Glioblastoma Cells J. Biol. Chem., August 10, 2001; 276(33): 30819 - 30826. [Abstract] [Full Text] [PDF]