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J. Biol. Chem., Vol. 261, Issue 19, 8597-8600, Jul, 1986
J Preiss, CR Loomis, WR Bishop, R Stein, JE Niedel and RM Bell
A simple enzymatic method for the quantitation of the mass of sn-1,2-
diacylglycerol (DAG) present in crude lipid extracts was developed to
assess the function of DAGs as intracellular "second messengers" of
extracellular agents and of oncogene products. The assay employed
Escherichia coli DAG kinase which constituted approximately 15% of the
membrane protein of a plasmid-bearing strain and defined mixed micellar
conditions to solubilize the DAG present and allow its quantitative
conversion to [32P]phosphatidic acid. The assay was proportional with the
amount of DAG added over the range of 25 pmol to 25 nmol. The rapid rise of
DAG in platelets stimulated with thrombin (210% over basal) and in
hepatocytes stimulated with vasopressin (230% over basal) was quantitated
and the values agreed with previous measurements. The amounts of DAG in
normal rat kidney (NRK) cells grown at 34 and 38 degrees C, respectively,
were 0.47 and 0.61 nmol/100 nmol of phospholipid. In K-ras-transformed NRK
cells grown at 34 or 38 degrees C, DAG levels were elevated 168 or 138%,
respectively. When a temperature-sensitive K-ras NRK cell line was
investigated, the amount of DAG present was elevated at the permissive but
not at the restrictive temperature. These data are consistent with the
K-ras protein functioning in transmembrane signalling by activating
phospholipase C. Protein kinase C (Ca2+/phospholipid-dependent enzyme)
activation by DAG may play an important role in cellular transformation.
Quantitative measurement of sn-1,2-diacylglycerols present in platelets, hepatocytes, and ras- and sis-transformed normal rat kidney cells
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|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 G. Liu, M. H. Ghahremani, B. Banihashemi, and P. R. Albert Diacylglycerol and ceramide formation induced by dopamine D2S receptors via Gbeta gamma -subunits in Balb/c-3T3 cells Am J Physiol Cell Physiol, March 1, 2003; 284(3): C640 - C648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Czarny, J. Liu, P. Oh, and J. E. Schnitzer Transient Mechanoactivation of Neutral Sphingomyelinase in Caveolae to Generate Ceramide J. Biol. Chem., February 7, 2003; 278(7): 4424 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
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|
|
|
|
|
|
V. Hinkovska-Galcheva, L. Boxer, P. J. Mansfield, A. D. Schreiber, and J. A. Shayman Enhanced Phagocytosis through Inhibition of de Novo Ceramide Synthesis J. Biol. Chem., January 3, 2003; 278(2): 974 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
L. Shu, L. Lee, and J. A. Shayman Regulation of Phospholipase C-gamma Activity by Glycosphingolipids J. Biol. Chem., May 17, 2002; 277(21): 18447 - 18453. [Abstract] [Full Text] [PDF]
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