|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 261, Issue 2, 538-547, Jan, 1986
PA Singer, K Trevor and RG Oshima
A cDNA clone of a keratin-related, intermediate filament protein,
designated Endo B, was constructed from size-fractionated parietal
endodermal mRNA and characterized. The 1466-nucleotide cDNA insert contains
an open reading frame of 1272 nucleotides that would result in 5' and 3'
noncoding sequences of 54 and 60 nucleotides, respectively. The predicted
amino acid composition, molecular weight (47,400), and peptide pattern
correlate well with data obtained on the isolated protein. The predicted
amino acid sequence fits easily into the general domain structure suggested
for all intermediate filament proteins with a unique amino-terminal head
domain, a large conserved central domain of predominantly alpha-helical
structure, and a relatively unique carboxyl-terminal or tail domain. Over
the entire molecule, Endo B is 43% identical with human 52-kDa epidermal
type I keratin. However, over two of the three regions contained in the
central domain that are predicted to form coiled-coil structures, the Endo
B is 54-68% identical with other type I keratin sequences. This homology,
along with the presence of the completely conserved sequence
DNARLAADDFR-KYE, which is found in all type I keratins, permits the
unambiguous identification of Endo B as a type I keratin. Comparison of the
Endo B sequence to other intermediate filament proteins reveals 22 residues
which are identical in all intermediate filament proteins regardless of
whether filament formation requires only one type of protein subunit
(vimentin, desmin, glial fibrillar acidic protein, or a neurofilament
protein) or two dissimilar types (type I and type II keratins). Endo B mRNA
was detectable in RNA isolated from F9 cells treated with retinoic acid for
48 h. Approximately three to five genes homologous to Endo B were detected
in the mouse genome.
Molecular cloning and characterization of the Endo B cytokeratin expressed in preimplantation mouse embryos
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kingsley-Kallesen, S. S. Mukhopadhyay, S. L. Wyszomierski, S. Schanler, G. Schutz, and J. M. Rosen The Mineralocorticoid Receptor May Compensate for the Loss of the Glucocorticoid Receptor at Specific Stages of Mammary Gland Development Mol. Endocrinol., September 1, 2002; 16(9): 2008 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Miyoshi, J. M. Shillingford, G. H. Smith, S. L. Grimm, K.-U. Wagner, T. Oka, J. M. Rosen, G. W. Robinson, and L. Hennighausen Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium J. Cell Biol., November 12, 2001; 155(4): 531 - 542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Robertson, M Kennedy, J. Shannon, and G Keller A transitional stage in the commitment of mesoderm to hematopoiesis requiring the transcription factor SCL/tal-1 Development, January 6, 2000; 127(11): 2447 - 2459. [Abstract] [PDF]
|
|
|
|
|
|
R. Eferl, M. Sibilia, F. Hilberg, A. Fuchsbichler, I. Kufferath, B. Guertl, R. Zenz, E. F. Wagner, and K. Zatloukal Functions of c-Jun in Liver and Heart Development J. Cell Biol., May 31, 1999; 145(5): 1049 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Spagnoli, L. Amicone, M. Tripodi, and M. C. Weiss Identification of a Bipotential Precursor Cell in Hepatic Cell Lines Derived from Transgenic Mice Expressing Cyto-Met in the Liver J. Cell Biol., November 16, 1998; 143(4): 1101 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. Robinson, P. F. Johnson, L. Hennighausen, and E. Sterneck The C/EBPbeta transcription factor regulates epithelial cell proliferation and differentiation in the mammary gland Genes & Dev., June 15, 1998; 12(12): 1907 - 1916. [Abstract] [Full Text]
|
|
|
|
|
|
G. F. Spath and M. C. Weiss Hepatocyte Nuclear Factor 4 Provokes Expression of Epithelial Marker Genes, Acting As a Morphogen in Dedifferentiated Hepatoma Cells J. Cell Biol., February 23, 1998; 140(4): 935 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Caulin, G. S. Salvesen, and R. G. Oshima Caspase Cleavage of Keratin 18 and Reorganization of Intermediate Filaments during Epithelial Cell Apoptosis J. Cell Biol., September 22, 1997; 138(6): 1379 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Post, P Souza, J Liu, I Tseu, J Wang, M Kuliszewski, and A. Tanswell Keratinocyte growth factor and its receptor are involved in regulating early lung branching Development, January 10, 1996; 122(10): 3107 - 3115. [Abstract] [PDF]
|
|
|
|
|
|
E Hermesz, S Mackem, and K. Mahon Rpx: a novel anterior-restricted homeobox gene progressively activated in the prechordal plate, anterior neural plate and Rathke's pouch of the mouse embryo Development, January 1, 1996; 122(1): 41 - 52. [Abstract] [PDF]
|
|
|
|
|
|
H Baribault, J Price, K Miyai, and R G Oshima Mid-gestational lethality in mice lacking keratin 8. Genes & Dev., July 1, 1993; 7(7a): 1191 - 1202. [Abstract] [PDF]
|
|
|
|
|
|
A S Espeseth, S P Murphy, and E Linney Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells. Genes & Dev., November 1, 1989; 3(11): 1647 - 1656. [Abstract] [PDF]
|
|
|
|
|
|
S E LaFlamme, M Jamrich, K Richter, T D Sargent, and I B Dawid Xenopus endo B is a keratin preferentially expressed in the embryonic notochord. Genes & Dev., July 1, 1988; 2(7): 853 - 862. [Abstract] [PDF]
|
|
|
|
|
|
R G Oshima, K Trevor, L H Shevinsky, O A Ryder, and G Cecena Identification of the gene coding for the Endo B murine cytokeratin and its methylated, stable inactive state in mouse nonepithelial cells. Genes & Dev., May 1, 1988; 2(5): 505 - 516. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |