HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mullenbach, G. T. Articles by Steimer, K. S. Search for Related Content PubMed PubMed Citation Articles by Mullenbach, G. T. Articles by Steimer, K. S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 2, 719-722, Jan, 1986

Chemical synthesis and expression in yeast of a gene encoding connective tissue activating peptide-III. A novel approach for the facile assembly of a gene encoding a human platelet-derived mitogen

GT Mullenbach, A Tabrizi, RW Blacher and KS Steimer

A synthetic gene encoding the platelet-derived factor, connective tissue activating peptide-III (CTAP-III) (Castor, C.W., Miller, J.W., and Walz, D.A. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 765-769), has been expressed and secreted from Saccharomyces cerevisiae by using a yeast expression vector and an alpha-factor leader segment. Mitogenic activity reported for naturally derived CTAP-III has been demonstrated here for recombinant CTAP-III. Active CTAP-III has been purified to apparent homogeneity. Structural studies have confirmed its identity. A general approach for the facile synthesis of genes is presented which has permitted the assembly of the entire structural gene and flanking regions (280 base pairs) from 20 oligomers in a single annealing and ligation reaction pool.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. M. Pillai, M. Iwata, N. Awaya, L. Graf, and B. Torok-Storb Monocyte-derived CXCL7 peptides in the marrow microenvironment Blood, May 1, 2006; 107(9): 3520 - 3526. [Abstract] [Full Text] [PDF]

				S. Struyf, P. Proost, J.-P. Lenaerts, G. Stoops, A. Wuyts, and J. Van Damme Identification of a blood-derived chemoattractant for neutrophils and lymphocytes as a novel CC chemokine, Regakine-1 Blood, April 15, 2001; 97(8): 2197 - 2204. [Abstract] [Full Text] [PDF]

				B. J. Rollins Chemokines Blood, August 1, 1997; 90(3): 909 - 928. [Full Text] [PDF]

				K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, and H. Erlich Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction Cold Spring Harb Symp Quant Biol, January 1, 1986; 51(0): 263 - 273. [Abstract] [PDF]