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J. Biol. Chem., Vol. 261, Issue 20, 9079-9082, 07, 1986

Different substrate specificities of the two DNA ligases of mammalian cells

JE Arrand, AE Willis, I Goldsmith and T Lindahl

Mammalian cells contain the DNA ligases I and II. These enzymes show different molecular weights and heat labilities, and antibodies against ligase I do not inhibit ligase II. Here, the nonidentical substrate specificities of the enzymes are described. Under standard reaction conditions DNA ligase I, but not ligase II, catalyzes blunt-end joining of DNA, while ligase II is the only activity that joins oligo(dT) molecules hydrogen-bonded to poly(rA). These differences facilitate the distinction between the two enzymes and should permit further analysis of their functions.
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