|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 261, Issue 20, 9109-9115, 07, 1986
T Hayakawa, K Suzuki, S Suzuki, PC Andrews and BM Babior
Two-dimensional gel electrophoresis was used to study protein
phosphorylation in granules, membranes, and soluble fractions from human
neutrophils that had been loaded with 32Pi. In resting cells, label was
incorporated primarily into proteins of the membranes and the soluble
supernatant; little appeared in the granules. Activation of 32P- loaded
neutrophils resulted in an increase in the 32P content of a small number of
membrane and soluble proteins without a change in the labeling of the
granule fraction. The identity of the proteins affected by activation
depended on the activating agent used; all of the activating agents,
however, caused an increase in the labeling of a group of approximately
48-kDa proteins that appeared to be distributed between the membranes and
the soluble supernatant. To investigate the role of phosphorylation in the
activation of the respiratory burst oxidase, the incorporation of 32P into
phosphoproteins was studied in neutrophils from patients with chronic
granulomatous disease. When these cells were exposed to phorbol myristate
acetate, one of the agents used for the activation of normal neutrophils,
the 48-kDa proteins in the membranes and supernatants failed to take up
additional 32P. Phosphorylation patterns in normal neutrophils activated
under nitrogen were similar to the patterns seen with cells activated in
air, suggesting that the differences in phosphorylation between normal and
chronic granulomatous disease neutrophils did not represent secondary
effects of the oxidants produced by the normal cells, but reflected primary
biochemical differences between the normal and the defective phagocytes. We
postulate from these results that the uptake of phosphate by the 48-kDa
protein group may be involved in the activation of the respiratory burst
oxidase.
A possible role for protein phosphorylation in the activation of the respiratory burst in human neutrophils. Evidence from studies with cells from patients with chronic granulomatous disease
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Oshizawa, T. Yamaguchi, K. Suzuki, Y. Yamamoto, and T. Hayakawa Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase J. Biochem., December 1, 2003; 134(6): 827 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M.-C. Dang, A. R. Cross, M. T. Quinn, and B. M. Babior Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558 II PNAS, April 2, 2002; 99(7): 4262 - 4265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ochi, T. Miyawaki, H. Matsuda, M. Oda, M. Nagahama, and J. Sakurai Clostridium perfringens{alpha}-toxin induces rabbit neutrophil adhesion Microbiology, January 1, 2002; 148(1): 237 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M.-C. Dang, A. R. Cross, and B. M. Babior Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558 PNAS, March 13, 2001; 98(6): 3001 - 3005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M.-C. Dang, A. Fontayne, J. Hakim, J. El Benna, and A. Perianin Protein Kinase C {{zeta}} Phosphorylates a Subset of Selective Sites of the NADPH Oxidase Component p47phox and Participates in Formyl Peptide-Mediated Neutrophil Respiratory Burst J. Immunol., January 15, 2001; 166(2): 1206 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Lopes, C. R. Hoyal, U. G. Knaus, and B. M. Babior Activation of the Leukocyte NADPH Oxidase by Protein Kinase C in a Partially Recombinant Cell-free System J. Biol. Chem., May 28, 1999; 274(22): 15533 - 15537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-A. H. Allen, F. R. DeLeo, A. Gallois, S. Toyoshima, K. Suzuki, and W. M. Nauseef Transient Association of the Nicotinamide Adenine Dinucleotide Phosphate Oxidase Subunits p47phox and p67phox With Phagosomes in Neutrophils From Patients With X-Linked Chronic Granulomatous Disease Blood, May 15, 1999; 93(10): 3521 - 3530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li, V. Subbulakshmi, A. P. Fields, N. R. Murray, and M. K. Cathcart Protein Kinase Calpha Regulates Human Monocyte Obardot 2 Production and Low Density Lipoprotein Lipid Oxidation J. Biol. Chem., February 5, 1999; 274(6): 3764 - 3771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Johnson, J.-W. Park, J. E. Benna, L. P. Faust, O. Inanami, and B. M. Babior Activation of p47PHOX, a Cytosolic Subunit of the Leukocyte NADPH Oxidase. PHOSPHORYLATION OF Ser-359 OR Ser-370 PRECEDES PHOSPHORYLATION AT OTHER SITES AND IS REQUIRED FOR ACTIVITY J. Biol. Chem., December 25, 1998; 273(52): 35147 - 35152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Morozov, O. Lotan, G. Joseph, Y. Gorzalczany, and E. Pick Mapping of Functional Domains in p47phox Involved in the Activation of NADPH Oxidase by "Peptide Walking" J. Biol. Chem., June 19, 1998; 273(25): 15435 - 15444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Inanami, J. L. Johnson, J. K. McAdara, J. E. Benna, L. R. P. Faust, P. E. Newburger, and B. M. Babior Activation of the Leukocyte NADPH Oxidase by Phorbol Ester Requires the Phosphorylation of p47PHOX on Serine 303 or 304 J. Biol. Chem., April 17, 1998; 273(16): 9539 - 9543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhen, L. Yu, and M. C. Dinauer Probing the Role of the Carboxyl Terminus of the gp91phox Subunit of Neutrophil Flavocytochrome b558 using Site-directed Mutagenesis J. Biol. Chem., March 13, 1998; 273(11): 6575 - 6581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Swain, S. L. Helgerson, A. R. Davis, L. K. Nelson, and M. T. Quinn Analysis of Activation-induced Conformational Changes in p47phox Using Tryptophan Fluorescence Spectroscopy J. Biol. Chem., November 21, 1997; 272(47): 29502 - 29510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-W. Park, C. R.. Hoyal, J. E. Benna, and B. M. Babior Kinase-dependent Activation of the Leukocyte NADPH Oxidase in a Cell-free System. PHOSPHORYLATION OF MEMBRANES AND p47PHOX DURING OXIDASE ACTIVATION J. Biol. Chem., April 25, 1997; 272(17): 11035 - 11043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. De Leo, K. V. Ulman, A. R. Davis, K. L. Jutila, and M. T. Quinn Assembly of the Human Neutrophil NADPH Oxidase Involves Binding of p67phox and Flavocytochrome b to a Common Functional Domain in p47phox J. Biol. Chem., July 19, 1996; 271(29): 17013 - 17020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Benna, L. R. P. Faust, J. L. Johnson, and B. M. Babior Phosphorylation of the Respiratory Burst Oxidase Subunit p47[IMAGE] as Determined by Two-dimensional Phosphopeptide Mapping J. Biol. Chem., March 15, 1996; 271(11): 6374 - 6378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. DeLeo, W. M. Nauseef, A. J. Jesaitis, J. B. Burritt, R. A. Clark, and M. T. Quinn A Domain of p47[IMAGE] That Interacts with Human Neutrophil Flavocytochrome b[IMAGE] J. Biol. Chem., November 3, 1995; 270(44): 26246 - 26251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Suzuki, T. Yamaguchi, T. Tanaka, T. Kawanishi, T. Nishimaki-Mogami, K. Yamamoto, T. Tsuji, T. Irimura, T. Hayakawa, and A. Takahashi Activation Induces Dephosphorylation of Cofilin and Its Translocation to Plasma Membranes in Neutrophil-like Differentiated HL-60 Cells J. Biol. Chem., August 18, 1995; 270(33): 19551 - 19556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lomax, T. Leto, H Nunoi, J. Gallin, and H. Malech Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease Science, July 28, 1989; 245(4916): 409 - 412. [Abstract] [PDF]
|
|
|
|
|
|
H Nunoi, D Rotrosen, J. Gallin, and H. Malech Two forms of autosomal chronic granulomatous disease lack distinct neutrophil cytosol factors Science, December 2, 1988; 242(4883): 1298 - 1301. [Abstract] [PDF]
|
|
|
|
|
|
P. M.-C. Dang, A. R. Cross, M. T. Quinn, and B. M. Babior Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558 II PNAS, April 2, 2002; 99(7): 4262 - 4265. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |