HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doyle, J. J. Articles by Slightom, J. L. Search for Related Content PubMed PubMed Citation Articles by Doyle, J. J. Articles by Slightom, J. L. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 20, 9228-9238, 07, 1986

The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. Structural homologies of genes and proteins

JJ Doyle, MA Schuler, WD Godette, V Zenger, RN Beachy and JL Slightom

Considerable information is now available concerning the 7 S seed storage proteins of legumes and the genes that encode them. Our study compares the gene encoding a beta-type subunit of phaseolin (Pvu beta), the 7 S protein of common bean (Phaseolus vulgaris), with the gene encoding an alpha'-subunit of beta-conglycinin (Gma alpha'), the 7 S protein of soybean (Glycine max). The comparison involves 2880 base pairs of Pvu beta and 3636 base pairs of Gma alpha' and includes approximately 1 kilobase pair of 5'-flanking sequences, and 5' and 3' untranslated sequences, as well as the six exons and five introns that are found to occur in similar positions in both genes. Conserved sequences in the 5'-flanking regions of these genes are discussed in light of their potential regulatory role. Published sequences for 7 S genes of pea (Pisum sativum) permit the inference of the nature and direction of evolutionary change and, in particular, show that the major size difference between the large Gma alpha' polypeptide and the smaller polypeptides of pea and common bean is due to a large insertion in the first exon of Gma alpha'. Comparisons of protein primary structure, potential glycosylation sites, and predicted protein hydropathy show that strongly conserved features of 7 S proteins cut across exon boundaries and that nonconserved regions exist that may have potential for protein modification.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Sriram, D. B. Fulton, V. V. Iyer, J. M. Peterson, R. Zhou, M. E. Westgate, M. H. Spalding, and J. V. Shanks Quantification of Compartmented Metabolic Fluxes in Developing Soybean Embryos by Employing Biosynthetically Directed Fractional 13C Labeling, Two-Dimensional [13C, 1H] Nuclear Magnetic Resonance, and Comprehensive Isotopomer Balancing Plant Physiology, October 1, 2004; 136(2): 3043 - 3057. [Abstract] [Full Text] [PDF]

				T. Nishi, H. Hara, K. Asano, and F. Tomita The Soybean {beta}-Conglycinin {beta} 51-63 Fragment Suppresses Appetite by Stimulating Cholecystokinin Release in Rats J. Nutr., August 1, 2003; 133(8): 2537 - 2542. [Abstract] [Full Text] [PDF]

				D. Yamauchi Regulation of Gene Expression of a Cysteine Proteinase, EP-C1, by a VIVIPAROUS1-like Factor from Common Bean Plant Cell Physiol., June 15, 2003; 44(6): 649 - 652. [Abstract] [Full Text] [PDF]

				E. M. Herman, R. M. Helm, R. Jung, and A. J. Kinney Genetic Modification Removes an Immunodominant Allergen from Soybean Plant Physiology, May 1, 2003; 132(1): 36 - 43. [Abstract] [Full Text] [PDF]

				E. B. Cahoon, K. G. Ripp, S. E. Hall, and B. McGonigle Transgenic Production of Epoxy Fatty Acids by Expression of a Cytochrome P450 Enzyme from Euphorbia lagascae Seed Plant Physiology, February 1, 2002; 128(2): 615 - 624. [Abstract] [Full Text] [PDF]

				E. B. Cahoon, E.-F. Marillia, K. L. Stecca, S. E. Hall, D. C. Taylor, and A. J. Kinney Production of Fatty Acid Components of Meadowfoam Oil in Somatic Soybean Embryos Plant Physiology, September 1, 2000; 124(1): 243 - 252. [Abstract] [Full Text]

				E. B. Cahoon, T. J. Carlson, K. G. Ripp, B. J. Schweiger, G. A. Cook, S. E. Hall, and A. J. Kinney Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos PNAS, October 26, 1999; 96(22): 12935 - 12940. [Abstract] [Full Text] [PDF]

				P. J. Overvoorde, W. S. Chao, and H. D. Grimes A Plasma Membrane Sucrose-binding Protein That Mediates Sucrose Uptake Shares Structural and Sequence Similarity with Seed Storage Proteins but Remains Functionally Distinct J. Biol. Chem., June 20, 1997; 272(25): 15898 - 15904. [Abstract] [Full Text] [PDF]

				R. KAY, A. CHAN, M. DALY, and J. MCPHERSON Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes Science, June 5, 1987; 236(4806): 1299 - 1302. [Abstract] [PDF]

				E. B. Cahoon, K. G. Ripp, S. E. Hall, and A. J. Kinney Formation of Conjugated Delta 8,Delta 10-Double Bonds by Delta 12-Oleic-acid Desaturase-related Enzymes. BIOSYNTHETIC ORIGIN OF CALENDIC ACID J. Biol. Chem., January 19, 2001; 276(4): 2637 - 2643. [Abstract] [Full Text] [PDF]