|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 261, Issue 20, 9274-9281, Jul, 1986
J Northrop, A Weber, MS Mooseker, C Franzini-Armstrong, MF Bishop, GR Dubyak, M Tucker and TP Walsh
The concentration of ionized calcium required for the capping of barbed
filament ends by villin is about 4 orders of magnitude lower than that
required for the cutting activity of villin. Capping was 50% complete at
about 10-30 nM Ca2+, a level expected in resting cells, whereas the cutting
rate was half-maximal at about 200 microM, making it possible to completely
separate filament capping from filament cutting. Analysis of capping in
terms of coupled equilibria between calcium binding to villin and
calcium-villin binding to the barbed ends of actin filaments gives a value
of 10(16)-10(17) M-2 for the product of the two binding constants. By
comparison the binding constant reported for the rapidly exchanging calcium
sites on villin is 2 X 10(5) M-1 and that for binding of calcium-saturated
villin to barbed ends has a minimum value of 10(11) M-1 giving a product of
2 X 10(16) M-1. The close similarity of the two sets of values suggests
that capping is regulated by the rapidly exchanging calcium sites on
villin. In terms of coupled equilibria the calcium requirement for filament
capping decreases with increasing concentrations of free villin. The scant
information on the mechanism of cutting allows only an estimate of the
maximal value for the calcium-binding constant of the site regulating
cutting which is about 2-5 X 10(3) M-1. Cutting is followed by rapid
capping of the newly released barbed ends.
Different calcium dependence of the capping and cutting activities of villin
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Revenu, M. Courtois, A. Michelot, C. Sykes, D. Louvard, and S. Robine Villin Severing Activity Enhances Actin-based Motility In Vivo Mol. Biol. Cell, March 1, 2007; 18(3): 827 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tomar, S. George, P. Kansal, Y. Wang, and S. Khurana Interaction of Phospholipase C-{gamma}1 with Villin Regulates Epithelial Cell Migration J. Biol. Chem., October 20, 2006; 281(42): 31972 - 31986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lian, Y. Wang, J. Draznin, D. Eslin, J. S. Bennett, M. Poncz, D. Wu, and C. S. Abrams The relative role of PLC{beta} and PI3K{gamma} in platelet activation Blood, July 1, 2005; 106(1): 110 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, R. C. Robinson, L. Y. Gao, T. Matsumoto, A. Brunet, L. Blanchoin, and C. J. Staiger Arabidopsis VILLIN1 Generates Actin Filament Cables That Are Resistant to Depolymerization PLANT CELL, February 1, 2005; 17(2): 486 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tomar, Y. Wang, N. Kumar, S. George, B. Ceacareanu, A. Hassid, K. E. Chapman, A. M. Aryal, C. M. Waters, and S. Khurana Regulation of Cell Motility by Tyrosine Phosphorylated Villin Mol. Biol. Cell, November 1, 2004; 15(11): 4807 - 4817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kumar, A. Tomar, A. L. Parrill, and S. Khurana Functional Dissection and Molecular Characterization of Calcium-sensitive Actin-capping and Actin-depolymerizing Sites in Villin J. Biol. Chem., October 22, 2004; 279(43): 45036 - 45046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kumar and S. Khurana Identification of a Functional Switch for Actin Severing by Cytoskeletal Proteins J. Biol. Chem., June 11, 2004; 279(24): 24915 - 24918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, L. Blanchoin, F. Chaudhry, V. E. Franklin-Tong, and C. J. Staiger A Gelsolin-like Protein from Papaver rhoeas Pollen (PrABP80) Stimulates Calcium-regulated Severing and Depolymerization of Actin Filaments J. Biol. Chem., May 28, 2004; 279(22): 23364 - 23375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kumar, P. Zhao, A. Tomar, C. A. Galea, and S. Khurana Association of Villin with Phosphatidylinositol 4,5-Bisphosphate Regulates the Actin Cytoskeleton J. Biol. Chem., January 23, 2004; 279(4): 3096 - 3110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Athman, D. Louvard, and S. Robine Villin Enhances Hepatocyte Growth Factor-induced Actin Cytoskeleton Remodeling in Epithelial Cells Mol. Biol. Cell, November 1, 2003; 14(11): 4641 - 4653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Fowler, N. J. Greenfield, and J. Moyer Tropomodulin Contains Two Actin Filament Pointed End-capping Domains J. Biol. Chem., October 10, 2003; 278(41): 40000 - 40009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Athman, D. Louvard, and S. Robine The Epithelial Cell Cytoskeleton and Intracellular Trafficking: III. How is villin involved in the actin cytoskeleton dynamics in intestinal cells? Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G496 - G502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Weber, C. R. Pennise, and V. M. Fowler Tropomodulin Increases the Critical Concentration of Barbed End-capped Actin Filaments by Converting ADP{middle dot}Pi-actin to ADP-actin at All Pointed Filament Ends J. Biol. Chem., December 3, 1999; 274(49): 34637 - 34645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ferrary, M. Cohen-Tannoudji, G. Pehau-Arnaudet, A. Lapillonne, R. Athman, T. Ruiz, L. Boulouha, F. El Marjou, A. Doye, J.-J. Fontaine, et al. In Vivo, Villin Is Required for Ca2+-dependent F-actin Disruption in Intestinal Brush Borders J. Cell Biol., August 23, 1999; 146(4): 819 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Feng, E. Kim, W.-L. Lee, C. J. Miller, B. Kuang, E. Reisler, and P. A. Rubenstein Fluorescence Probing of Yeast Actin Subdomain 3/4 Hydrophobic Loop 262-274. ACTIN-ACTIN AND ACTIN-MYOSIN INTERACTIONS IN ACTIN FILAMENTS J. Biol. Chem., July 4, 1997; 272(27): 16829 - 16837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Freeman, Z. Chen, J. Horenstein, A. Weber, and J. Field An Actin Monomer Binding Activity Localizes to the Carboxyl-terminal Half of the Saccharomyces cerevisiae Cyclase-associated Protein J. Biol. Chem., March 10, 1995; 270(10): 5680 - 5685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Bubb, I. Spector, A. D. Bershadsky, and E. D. Korn Swinholide A Is a Microfilament Disrupting Marine Toxin That Stabilizes Actin Dimers and Severs Actin Filaments J. Biol. Chem., February 24, 1995; 270(8): 3463 - 3466. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |