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J. Biol. Chem., Vol. 261, Issue 21, 9587-9590, 07, 1986
JD Robishaw, MD Smigel and AG Gilman
Most cells contain two forms of the alpha subunit of the G protein (Gs)
that stimulates adenylate cyclase; their apparent molecular weights are
45,000 and 52,000. Two cDNAs that correspond to distinct mRNAs for the
alpha subunit of Gs have been cloned from a bovine adrenal library and
sequenced. The sequences of the two cDNAs, designated pGs-l and pGs-S, are
identical except for a single stretch of 46 nucleotides in the coding
region, where four are altered and 42 are deleted in pGs-S. Expression of
pGs-S and pGs-l in COS-m6 cells yields protein products with apparent
molecular weights of 45,000 and 52,000, respectively, based on their
mobility in sodium dodecyl sulfate-polyacrylamide gels. We conclude that
pGs-S and pGs-l encode the 45- and 52-kDa forms of Gs alpha, respectively,
and propose that the mRNAs encoding these proteins arise from a single gene
by internal alternative RNA splicing.
Molecular basis for two forms of the G protein that stimulates adenylate cyclase
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