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J. Biol. Chem., Vol. 261, Issue 21, 9703-9709, 07, 1986
SR Ellis, MJ Morales, JM Li, AK Hopper and NC Martin
The trm1 mutation of Saccharomyces cerevisiae is a single nuclear mutation
that affects a specific base modification of both cytoplasmic and
mitochondrial tRNA. Transfer RNA isolated from trm1 cells lacks the
modified base N2,N2-dimethylguanosine, and extracts from these cells do not
have detectable N2,N2-dimethylguanosine-specific tRNA methyltransferase
activity. As part of our efforts to determine how this mutation affects
enzyme activities in two different cellular compartments we have isolated
the TRM1 locus by genetic complementation. The TRM1 locus restores the
N2,N2-dimethylguanosine modification to both cytoplasmic and mitochondrial
tRNA in trm1 cells. An open reading frame in this TRM1 gene is essential
for complementation of the trm1 phenotype. Expression of this open reading
frame in Escherichia coli converts the organism from one that neither makes
N2,N2-dimethylguanosine nor has N2,N2-dimethylguanosine-specific tRNA
methyltransferase activity into one that does. This result suggests that
the TRM1 locus is the structural gene for the tRNA modification enzyme and
that both nuclear/cytoplasmic and mitochondrial forms of the
methyltransferase are produced from the same gene.
Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae
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