HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coletta, M. Articles by Traylor, T. G. Search for Related Content PubMed PubMed Citation Articles by Coletta, M. Articles by Traylor, T. G. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 21, 9811-9814, 07, 1986

pH dependence of carbon monoxide binding to ferrous horseradish peroxidase

M Coletta, F Ascoli, M Brunori and TG Traylor

The kinetic parameters of the reaction of horseradish peroxidase with CO have been determined at pH values between 10 and 3. At pH 7.0 the CO binding equilibrium constant L was measured using submicromolar concentrations of horseradish peroxidase; the value obtained corresponds to the ratio of the association and dissociation kinetic constants as expected for a simple binding mechanism to a monomeric hemeprotein. The CO association rate constant is pH-independent below pH 7, whereas in going from pH 7 to pH 11 a 2-fold increase can be detected, as previously reported (Kertesz, D., Antonini, E., Brunori, M., Wyman, J., and Zito, R. (1965) Biochemistry 4, 2672-2676). On the other hand, CO dissociation displays a peculiar pH rate profile characterized by a progressive decrease from pH 10 to pH 5 and by a very marked increase as the pH is further lowered to pH congruent to 3. Furthermore, the rate of CO dissociation is markedly enhanced in peroxidase reconstituted with protoheme dimethyl ester, suggesting a role of the propionates in the regulation of this process.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Ciaccio, G. De Sanctis, S. Marini, F. Sinibaldi, R. Santucci, A. Arcovito, A. Bellelli, E. Ghibaudi, P. Ferrari Rosa, and M. Coletta Proton Linkage for CO Binding and Redox Properties of Bovine Lactoperoxidase Biophys. J., January 1, 2004; 86(1): 448 - 454. [Abstract] [Full Text] [PDF]