HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bittner, M. A. Articles by Neubig, R. R. Search for Related Content PubMed PubMed Citation Articles by Bittner, M. A. Articles by Neubig, R. R. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 22, 10182-10188, 08, 1986

Guanine nucleotide effects on catecholamine secretion from digitonin- permeabilized adrenal chromaffin cells

MA Bittner, RW Holz and RR Neubig

The nonhydrolyzable GTP analogue guanosine 5'-(beta, gamma- imido)triphosphate (GMP-PNP) produced an ATP-dependent but Ca2+- independent stimulation of [3H]norepinephrine release from permeabilized chromaffin cells. This stimulation of secretion was 25- 35% of the secretion induced by 10 microM Ca2+. A similar Ca2+- independent stimulation was produced by other non-hydrolyzable GTP analogues. No effect was seen with a variety of other nucleotides, including GTP. The GMP-PNP effect was specifically inhibited by low concentrations of guanine nucleotides. Addition of cAMP did not mimic the Ca2+-independent GMP-PNP effect, but did slightly enhance Ca2+- dependent secretion. Pretreatment with pertussis toxin had no effect on Ca2+-dependent secretion or on the GMP-PNP effect. There was no detectable diglyceride or inositol phosphate produced during GMP-PNP treatment, and addition of diglyceride and inositol trisphosphate did not induce secretion. Guanosine 5'-(beta-thio)diphosphate (GDP-beta-S), in addition to its ability to inhibit the GMP-PNP effect, partially inhibited Ca2+-dependent secretion. At 10 microM free Ca2+, the effects of GMP-PNP and Ca2+ were nonadditive. In fact, secretion in the presence of both GMP-PNP and 10 microM Ca2+ was slightly less than secretion due to Ca2+ alone. These data suggest that a guanine nucleotide-dependent process interacts in some way with one or more components of the normal Ca2+-dependent secretory pathway. However, it may not be an intrinsic part of the mechanism underlying Ca2+-dependent secretion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Li, L. Han, T.-C. Chou, Y. Fujita, L. Arunachalam, A. Xu, A. Wong, S.-K. Chiew, Q. Wan, L. Wang, et al. RalA and RalB Function as the Critical GTP Sensors for GTP-Dependent Exocytosis J. Neurosci., January 3, 2007; 27(1): 190 - 202. [Abstract] [Full Text] [PDF]

				L. Wang, G. Li, and S. Sugita RalA-Exocyst Interaction Mediates GTP-dependent Exocytosis J. Biol. Chem., May 7, 2004; 279(19): 19875 - 19881. [Abstract] [Full Text] [PDF]

				M. G. Mozhayeva, M. F. Matos, X. Liu, and E. T. Kavalali Minimum Essential Factors Required for Vesicle Mobilization at Hippocampal Synapses J. Neurosci., February 18, 2004; 24(7): 1680 - 1688. [Abstract] [Full Text] [PDF]

				R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]

				H. Caohuy and H. B. Pollard Protein Kinase C and Guanosine Triphosphate Combine to Potentiate Calcium-dependent Membrane Fusion Driven by Annexin 7 J. Biol. Chem., July 5, 2002; 277(28): 25217 - 25225. [Abstract] [Full Text] [PDF]

				S. Boehm and H. Kubista Fine Tuning of Sympathetic Transmitter Release via Ionotropic and Metabotropic Presynaptic Receptors Pharmacol. Rev., March 1, 2002; 54(1): 43 - 99. [Abstract] [Full Text] [PDF]

				C. Herr, N. Smyth, S. Ullrich, F. Yun, P. Sasse, J. Hescheler, B. Fleischmann, K. Lasek, K. Brixius, R. H. G. Schwinger, et al. Loss of Annexin A7 Leads to Alterations in Frequency-Induced Shortening of Isolated Murine Cardiomyocytes Mol. Cell. Biol., July 1, 2001; 21(13): 4119 - 4128. [Abstract] [Full Text] [PDF]

				D. R. Giovannucci, M. D. Hlubek, and E. L. Stuenkel Mitochondria Regulate the Ca2+-Exocytosis Relationship of Bovine Adrenal Chromaffin Cells J. Neurosci., November 1, 1999; 19(21): 9261 - 9270. [Abstract] [Full Text] [PDF]

				S. Kanwal, D. G. Lowe, and G. J. Trachte Intracellular Fragments of the Natriuretic Peptide Receptor-C (NPR-C) Attenuate Dopamine Efflux Endocrinology, March 1, 1999; 140(3): 1118 - 1124. [Abstract] [Full Text]

				S. Takida, B. J. Elmquist, and G. J. Trachte C-Type Natriuretic Peptide Attenuates Evoked Dopamine Efflux by Influencing Go{alpha} Hypertension, January 1, 1999; 33(1): 124 - 129. [Abstract] [Full Text] [PDF]

				F Darchen, J Senyshyn, W. Brondyk, D. Taatjes, R. Holz, J. Henry, J. Denizot, and I. Macara The GTPase Rab3a is associated with large dense core vesicles in bovine chromaffin cells and rat PC12 cells J. Cell Sci., January 4, 1995; 108(4): 1639 - 1649. [Abstract] [PDF]

				R. W. Holz, M. D. Hlubek, S. D. Sorensen, S. K. Fisher, T. Balla, S. Ozaki, G. D. Prestwich, E. L. Stuenkel, and M. A. Bittner A Pleckstrin Homology Domain Specific for Phosphatidylinositol 4,5-Bisphosphate (PtdIns-4,5-P2) and Fused to Green Fluorescent Protein Identifies Plasma Membrane PtdIns-4,5-P2 as Being Important in Exocytosis J. Biol. Chem., June 2, 2000; 275(23): 17878 - 17885. [Abstract] [Full Text] [PDF]

				M. A. Bittner and R. W. Holz Latrotoxin Stimulates Secretion in Permeabilized Cells by Regulating an Intracellular Ca2+- and ATP-dependent Event. A ROLE FOR PROTEIN KINASE C J. Biol. Chem., August 11, 2000; 275(33): 25351 - 25357. [Abstract] [Full Text] [PDF]

				H. Caohuy and H. B. Pollard Activation of Annexin 7 by Protein Kinase C in Vitro and in Vivo J. Biol. Chem., April 13, 2001; 276(16): 12813 - 12821. [Abstract] [Full Text] [PDF]