HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carruthers, A. Search for Related Content PubMed PubMed Citation Articles by Carruthers, A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 24, 11028-11037, 08, 1986

ATP regulation of the human red cell sugar transporter

A Carruthers

Purified human red blood cell sugar transport protein intrinsic tryptophan fluorescence is quenched by D-glucose and 4,6-ethylidene glucose (sugars that bind to the transport), phloretin and cytochalasin B (transport inhibitors), and ATP. Cytochalasin B-induced quenching is a simple saturable phenomenon with Kd app of 0.15 microM and maximum capacity of 0.85 cytochalasin B binding sites per transporter. Sugar- induced quenching consists of two saturable components characterized by low and high Kd app binding parameters. These binding sites appear to correspond to influx and efflux transport sites, respectively, and coexist within the transporter molecule. ATP-induced quenching is also a simple saturable process with Kd app of 50 microM. Indirect estimates suggest that the ratio of ATP-binding sites per transporter is 0.87:1. ATP reduces the low Kd app and increases the high Kd app for sugar- induced fluorescence quenching. This effect is half-maximal at 45 microM ATP. ATP produces a 4-fold reduction in Km and 2.4-fold reduction in Vmax for cytochalasin B-inhibitable D-glucose efflux from inside-out red cell membrane vesicles (IOVs). This effect on transport is half-maximal at 45 microM ATP. AMP, ADP, alpha, beta- methyleneadenosine 5'-triphosphate, and beta, gamma-methyleneadenosine 5'-triphosphate at 1 mM are without effect on efflux of D-glucose from IOVs. ATP modulation of Km for D-glucose efflux from IOVs is immediate in onset and recovery. ATP inhibition of Vmax for D-glucose exit is complete within 5-15 min and is only partly reversed following 30-min incubation in ATP-free medium. These findings suggest that the human red cell sugar transport protein contains a nucleotide-binding site(s) through which ATP modifies the catalytic properties of the transporter.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. K. Khera, C. H. Joiner, A. Carruthers, C. J. Lindsell, E. P. Smith, R. S. Franco, Y. R. Holmes, and R. M. Cohen Evidence for Interindividual Heterogeneity in the Glucose Gradient Across the Human Red Blood Cell Membrane and Its Relationship to Hemoglobin Glycation Diabetes, September 1, 2008; 57(9): 2445 - 2452. [Abstract] [Full Text] [PDF]

				J. M. Leitch and A. Carruthers ATP-dependent sugar transport complexity in human erythrocytes Am J Physiol Cell Physiol, February 1, 2007; 292(2): C974 - C986. [Abstract] [Full Text] [PDF]

				R. Dauterive, S. Laroux, R. C. Bunn, A. Chaisson, T. Sanson, and B. C. Reed C-terminal Mutations That Alter the Turnover Number for 3-O-Methylglucose Transport by GLUT1 and GLUT4 J. Biol. Chem., May 10, 1996; 271(19): 11414 - 11421. [Abstract] [Full Text] [PDF]

				J. C. Vera, A. M. Reyes, J. G. Cárcamo, F. V. Velásquez, C. I. Rivas, R. H. Zhang, P. Strobel, R. Iribarren, H. I. Scher, J. C. Slebe, et al. Genistein Is a Natural Inhibitor of Hexose and Dehydroascorbic Acid Transport through the Glucose Transporter, GLUT1 J. Biol. Chem., April 12, 1996; 271(15): 8719 - 8724. [Abstract] [Full Text] [PDF]