HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Higgins, T. J. Articles by Inglis, A. S. Search for Related Content PubMed PubMed Citation Articles by Higgins, T. J. Articles by Inglis, A. S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 24, 11124-11130, Aug, 1986

Gene structure, protein structure, and regulation of the synthesis of a sulfur-rich protein in pea seeds

TJ Higgins, PM Chandler, PJ Randall, D Spencer, LR Beach, RJ Blagrove, AA Kortt and AS Inglis

Two low molecular weight pea seed albumins (Mr approximately 6000 and approximately 4000) have been characterized by protein, cDNA, and gene sequencing. Both proteins are encoded by separate regions of the same mRNA species. The initial translation product is a preproprotein from which a signal sequence is removed co-translationally. The resultant proprotein (PA1) is then cleaved post-translationally to yield the mature form of the two albumins (PA1a and PA1b). Comparison of cDNA and protein sequences suggests that at least four different PA1 genes are expressed in the pea genome. Both PA1a and PA1b have an unusually high cysteine content (7.5 and 16.2%, respectively). Pea seeds developing under suboptimal levels of sulfur nutrient supply contain reduced levels of PA1 mRNA and accumulate greatly reduced levels of PA1a and PA1b in the mature seed. In vitro transcription studies showed that this reduced level of PA1 mRNA resulted from reduced post- transcriptional stability rather than an altered rate of transcription of the PA1 gene. In contrast, during normal seed development, the level of PA1 mRNA seems to be under transcriptional control. Sequence comparisons reveal some homology between PA1 and a number of low molecular weight proteins from seeds of a wide range of mono- and dicotyledonous plants.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Gallardo, C. Firnhaber, H. Zuber, D. Hericher, M. Belghazi, C. Henry, H. Kuster, and R. Thompson A Combined Proteome and Transcriptome Analysis of Developing Medicago truncatula Seeds: Evidence for Metabolic Specialization of Maternal and Filial Tissues Mol. Cell. Proteomics, December 1, 2007; 6(12): 2165 - 2179. [Abstract] [Full Text] [PDF]

				M. Le Gall, L. Quillien, J. Gueguen, H. Rogniaux, and B. Seve Identification of Dietary and Endogenous Ileal Protein Losses in Pigs by Immunoblotting and Mass Spectrometry J. Nutr., May 1, 2005; 135(5): 1215 - 1222. [Abstract] [Full Text] [PDF]

				K. Gallardo, C. Le Signor, J. Vandekerckhove, R. D. Thompson, and J. Burstin Proteomics of Medicago truncatula Seed Development Establishes the Time Frame of Diverse Metabolic Processes Related to Reserve Accumulation Plant Physiology, October 1, 2003; 133(2): 664 - 682. [Abstract] [Full Text] [PDF]

				F. Thibaud-Nissen, R. T. Shealy, A. Khanna, and L. O. Vodkin Clustering of Microarray Data Reveals Transcript Patterns Associated with Somatic Embryogenesis in Soybean Plant Physiology, May 1, 2003; 132(1): 118 - 136. [Abstract] [Full Text] [PDF]