HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kruithof, E. K. Articles by Bachmann, F. Search for Related Content PubMed PubMed Citation Articles by Kruithof, E. K. Articles by Bachmann, F. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 24, 11207-11213, 08, 1986

Purification and characterization of a plasminogen activator inhibitor from the histiocytic lymphoma cell line U-937

EK Kruithof, JD Vassalli, WD Schleuning, RJ Mattaliano and F Bachmann

We report the production, purification, characterization, and partial amino acid sequence of a plasminogen inhibitor (PA-I). The starting material is culture fluid from phorbol myristate 13-acetate-treated U- 937 cells and the isolation steps consist of preparative isoelectric focusing followed by affinity chromatography on Cibacron Blue- Sepharose. PA-I migrates as a closely spaced doublet of 47-kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and forms covalent complexes with urokinase and two-chain tissue-type plasminogen activator, displaying second order rate constants of 0.9 X 10(6) M-1 s- 1 and 0.2 X 10(6) M-1 s-1, respectively. Upon treatment with 1 M NH4OH, the covalent complexes were hydrolyzed, yielding a 35-kDa inhibitor fragment. A partial amino acid sequence of PA-I showed that it belongs to the antithrombin III family of inhibitors. PA-I is immunologically related to a PA-inhibitor from human placenta. mRNA from phorbol myristate 13-acetate-treated U-937 cells directed, in a rabbit reticulocyte derived cell-free system, the biosynthesis of only one 47- kDa protein that could be immunoprecipitated with anti-PA-I IgG, indicating that the two molecular forms of PA-I are the products of post-translational processing.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Buyru, J. Altinisik, C. B. Gurel, and T. Ulutin PCR-RFLP Detection of PAI-2 Variants in Myocardial Infarction Clinical and Applied Thrombosis/Hemostasis, October 1, 2003; 9(4): 333 - 336. [Abstract] [PDF]

				T. Kietzmann, A. Samoylenko, U. Roth, and K. Jungermann Hypoxia-inducible factor-1 and hypoxia response elements mediate the induction of plasminogen activator inhibitor-1 gene expression by insulin in primary rat hepatocytes Blood, February 1, 2003; 101(3): 907 - 914. [Abstract] [Full Text] [PDF]

				A. Samoylenko, U. Roth, K. Jungermann, and T. Kietzmann The upstream stimulatory factor-2a inhibits plasminogen activator inhibitor-1 gene expression by binding to a promoter element adjacent to the hypoxia-inducible factor-1 binding site Blood, May 1, 2001; 97(9): 2657 - 2666. [Abstract] [Full Text] [PDF]

				H.-M. Zhou, I. Bolon, A. Nichols, A. Wohlwend, and J.-D. Vassalli Overexpression of Plasminogen Activator Inhibitor Type 2 in Basal Keratinocytes Enhances Papilloma Formation in Transgenic Mice Cancer Res., February 1, 2001; 61(3): 970 - 976. [Abstract] [Full Text]

				S. H. Cho, S. W. Tam, S. Demissie-Sanders, S. A. Filler, and C. K. Oh Production of Plasminogen Activator Inhibitor-1 by Human Mast Cells and Its Possible Role in Asthma J. Immunol., September 15, 2000; 165(6): 3154 - 3161. [Abstract] [Full Text] [PDF]

				T. Kietzmann, U. Roth, and K. Jungermann Induction of the Plasminogen Activator Inhibitor-1 Gene Expression by Mild Hypoxia Via a Hypoxia Response Element Binding the Hypoxia-Inducible Factor-1 in Rat Hepatocytes Blood, December 15, 1999; 94(12): 4177 - 4185. [Abstract] [Full Text] [PDF]

				D. L. Williams, B. Risse, S. Kim, D. Saunders, S. Orlin, M. S. Baker, P. J. Jensen, and R. M. Lavker Plasminogen Activator Inhibitor Type 2 in Human Corneal Epithelium Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1669 - 1675. [Abstract] [Full Text] [PDF]

				K. M. Dougherty, J. M. Pearson, A. Y. Yang, R. J. Westrick, M. S. Baker, and D. Ginsburg The plasminogen activator inhibitor-2 gene is not required for normal murine development or survival PNAS, January 19, 1999; 96(2): 686 - 691. [Abstract] [Full Text] [PDF]

				D. N. Saunders, K. M. L. Buttigieg, A. Gould, V. McPhun, and M. S. Baker Immunological Detection of Conformational Neoepitopes Associated with the Serpin Activity of Plasminogen Activator Inhibitor Type-2 J. Biol. Chem., May 1, 1998; 273(18): 10965 - 10971. [Abstract] [Full Text] [PDF]

				S. Sheng, B. Truong, D. Fredrickson, R. Wu, A. B. Pardee, and R. Sager Tissue-type plasminogen activator is a target of the tumor suppressor gene maspin PNAS, January 20, 1998; 95(2): 499 - 504. [Abstract] [Full Text] [PDF]

				P. Mikus and T. Ny Intracellular Polymerization of the Serpin Plasminogen Activator Inhibitor Type 2 J. Biol. Chem., April 26, 1996; 271(17): 10048 - 10053. [Abstract] [Full Text] [PDF]

				P. A. Pemberton, D. T. Wong, H. L. Gibson, M. C. Kiefer, P. A. Fitzpatrick, R. Sager, and P. J. Barr The Tumor Suppressor Maspin Does Not Undergo the Stressed to Relaxed Transition or Inhibit Trypsin-like Serine Proteases J. Biol. Chem., June 30, 1995; 270(26): 15832 - 15837. [Abstract] [Full Text] [PDF]

				C. Chen, B Lyons-Giordano, G. Lazarus, and P. Jensen Differential expression of plasminogen activators and their inhibitors in an organotypic skin coculture system J. Cell Sci., January 9, 1993; 106(1): 45 - 53. [Abstract] [PDF]