HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wettenhall, R. E. Articles by Hardesty, B. Search for Related Content PubMed PubMed Citation Articles by Wettenhall, R. E. Articles by Hardesty, B. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 261, Issue 27, 12444-12447, Sep, 1986

The NH2-terminal sequence of the alpha and gamma subunits of eukaryotic initiation factor 2 and the phosphorylation site for the heme-regulated eIF-2 alpha kinase

RE Wettenhall, W Kudlicki, G Kramer and B Hardesty

Rabbit reticulocyte eukaryotic initiation factor 2 was phosphorylated with the heme-regulated alpha subunit of eukaryotic initiation factor 2 kinase, and then the individual subunits were resolved by reversed- phase high performance liquid chromatography. Phosphorylated and unphosphorylated forms of the alpha subunit also were well resolved. The NH2-terminal sequences of intact alpha and gamma subunits were determined. No sequence was obtained from the beta subunit, suggesting that it may have a blocked NH2-terminus. Overlapping tryptic and chymotryptic phosphopeptides from the NH2-terminal sequence of the alpha subunit of eukaryotic initiation factor 2 were used to establish the order of amino acids 1-52 and localized the phosphorylation site within the sequence: -Leu-Leu-Ser48-Glu-Leu-Ser51-. Subdigestion of a tryptic fragment with chymotrypsin generated only phosphopeptides that appeared to terminate at leucine 50, indicating phosphorylation at serine 48.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. S. Y. Ting, P. N. Kao, D. W. Chan, L. G. Lintott, and S. P. Lees-Miller DNA-dependent Protein Kinase Interacts with Antigen Receptor Response Element Binding Proteins NF90 and NF45 J. Biol. Chem., January 23, 1998; 273(4): 2136 - 2145. [Abstract] [Full Text] [PDF]

				M. Gross and S. Hessefort Purification and Characterization of a 66-kDa Protein from Rabbit Reticulocyte Lysate Which Promotes the Recycling of Hsp 70 J. Biol. Chem., July 12, 1996; 271(28): 16833 - 16841. [Abstract] [Full Text] [PDF]

				R. Pearson, R. Wettenhall, A. Means, D. Hartshorne, and B. Kemp Autoregulation of enzymes by pseudosubstrate prototopes: myosin light chain kinase Science, August 19, 1988; 241(4868): 970 - 973. [Abstract] [PDF]

				L. Suva, G. Winslow, R. Wettenhall, R. Hammonds, J. Moseley, H Diefenbach-Jagger, C. Rodda, B. Kemp, H Rodriguez, E. Chen, et al. A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression Science, August 21, 1987; 237(4817): 893 - 896. [Abstract] [PDF]