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J. Biol. Chem., Vol. 261, Issue 27, 12534-12537, Sep, 1986
MA Bourdon, M Shiga and E Ruoslahti
The yolk sac carcinoma cell line L2 secretes a chondroitin/dermatan sulfate
proteoglycan that has an Mr 10,000 core protein and carries an average of
14 glycosaminoglycan chains. The amino acid sequence of the mature core
protein has been determined from cloned cDNA (Bourdon, M. A., Oldberg, A.,
Pierschbacher, M., and Ruoslahti, E. (1985) Proc. Natl. Acad. Sci. U. S. A.
82, 1321-1325). From additional cDNA sequences described in this report we
have identified the prepro core protein precursor of the yolk sac carcinoma
chondroitin/dermatan sulfate proteoglycan. From the amino acid sequence of
the core protein precursor can be deduced the protein processing events in
the biosynthesis of the proteoglycan. The amino acid sequence shows that
the 104-amino acid mature core protein is processed from a 179-amino acid
prepro core protein precursor which, in addition to the mature core
protein, contains a 26-amino acid signal peptide as well as a 49- amino
acid propeptide. The molecular weight of the prepro core protein predicted
from the cDNA sequence (Mr = 18,600) was in good agreement with the
molecular weight of the in vitro translation product (Mr = 19,000) of
hybrid-selected mRNA. Accordingly, we have designated the proteoglycan core
protein PG19. Further analysis of the PG19 mRNA by RNA sequencing confirmed
the identification of the core protein translation initiation codon by
revealing stop codons in all three reading frames of the upstream mRNA
sequence. Primer extension analyses demonstrated that the 5' untranslated
sequence of the proteoglycan mRNA is approximately 220 nucleotides in
length, which, combined with the length of cDNA clones, accounts for the
entire length of the coding sequence of PG19 mRNA from L2 cells. The cDNA
sequences presented here establish the complete protein sequence of PG19
and provide evidence of polypeptide processing during the biosynthesis of
the proteoglycan core protein.
Identification from cDNA of the precursor form of a chondroitin sulfate proteoglycan core protein
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