|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 261, Issue 27, 12579-12585, 09, 1986
M Handa, K Titani, LZ Holland, JR Roberts and ZM Ruggeri
The glycoprotein Ib (GPIb), a two-chain integral platelet membrane protein,
acts as a receptor for von Willebrand factor. In order to obtain
information on the domain involved in this function, as well as on the
structural organization of GPIb, the protein has been purified and
submitted to limited proteolysis using three different enzymes. The
resulting fragments were topographically oriented by means of partial
NH2-terminal sequence analysis and immunological identification using
monoclonal antibodies. One of these antibodies (LJ-Ib1) inhibited the von
Willebrand factor-GPIb interaction completely, one (LJ-P3) partially, and
one (LJ-Ib10) had no inhibitory effect. Three distinct fragments, the
38-kDa fragment produced by Serratia marcescens protease as well as the 45-
and 35-kDa fragments produced by trypsin, had the same NH2 terminus as the
intact GPIb alpha-chain (apparent molecular mass = 140 kDa). These
fragments and the alpha-chain reacted with the inhibitory antibodies. On
the other hand, three fragments produced by Staphylococcus aureus V8
protease, one of 92 kDa similar to the previously described
"macroglycopeptide" and two others of 52 and 45 kDa, had NH2-terminal
sequences different from that of the GPIb alpha- chain and reacted only
with the noninhibitor monoclonal antibody LJIb10. Thus, the binding domain
for von Willebrand factor resides near the NH2 terminus of the GPIb
alpha-chain, whereas the carbohydrate-rich region is part of the innermost
portion of GPIb and does not appear to be involved in the von Willebrand
factor binding function.
The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Peng, M. C. Berndt, M. A. Cruz, and J. A. Lopez The {alpha}1 helix-{beta}13 strand spanning Leu214 to Val229 of platelet glycoprotein Ib{alpha} facilitates the interaction with von Willebrand factor: evidence from characterization of the epitope of monoclonal antibody AP1 Blood, December 15, 2004; 104(13): 3971 - 3978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sabri, M. Jandrot-Perrus, J. Bertoglio, R. W. Farndale, V. M.-D. Mas, N. Debili, and W. Vainchenker Differential regulation of actin stress fiber assembly and proplatelet formation by {alpha}2{beta}1 integrin and GPVI in human megakaryocytes Blood, November 15, 2004; 104(10): 3117 - 3125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kanaji, S. Russell, J. Cunningham, K. Izuhara, J. E. B. Fox, and J. Ware Megakaryocyte proliferation and ploidy regulated by the cytoplasmic tail of glycoprotein Ib{alpha} Blood, November 15, 2004; 104(10): 3161 - 3168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shimizu, T. Matsushita, T. Kondo, Y. Inden, T. Kojima, H. Saito, and M. Hirai Identification of the Amino Acid Residues of the Platelet Glycoprotein Ib (GPIb) Essential for the von Willebrand Factor Binding by Clustered Charged-to-Alanine Scanning Mutagenesis J. Biol. Chem., April 16, 2004; 279(16): 16285 - 16294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kanaji, T. Kanaji, K. Furihata, K. Kato, J. L. Ware, and T. J. Kunicki Convulxin Binds to Native, Human Glycoprotein Ib{alpha} J. Biol. Chem., October 10, 2003; 278(41): 39452 - 39460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ulrichts, K. Vanhoorelbeke, S. Cauwenberghs, S. Vauterin, H. Kroll, S. Santoso, and H. Deckmyn Von Willebrand Factor But Not {alpha}-Thrombin Binding to Platelet Glycoprotein Ib{alpha} Is Influenced by the HPA-2 Polymorphism Arterioscler. Thromb. Vasc. Biol., July 1, 2003; 23(7): 1302 - 1307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Sjobring, U. Ringdahl, and Z. M. Ruggeri Induction of platelet thrombi by bacteria and antibodies Blood, December 15, 2002; 100(13): 4470 - 4477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mazzucato, P. Pradella, M. R. Cozzi, L. De Marco, and Z. M. Ruggeri Sequential cytoplasmic calcium signals in a 2-stage platelet activation process induced by the glycoprotein Ibalpha mechanoreceptor Blood, September 26, 2002; 100(8): 2793 - 2800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakayama, T. Matsushita, Z. Dong, J. E. Sadler, S. Jorieux, C. Mazurier, D. Meyer, T. Kojima, and H. Saito Identification of the Regulatory Elements of the Human von Willebrand Factor for Binding to Platelet GPIb. IMPORTANCE OF STRUCTURAL INTEGRITY OF THE REGIONS FLANKED BY THE CYS1272-CYS1458 DISULFIDE BOND J. Biol. Chem., June 7, 2002; 277(24): 22063 - 22072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Tait, J.-F. Dong, J. A. Lopez, I. W. Dawes, and B. H. Chong Site-directed mutagenesis of platelet glycoprotein Ibalpha demonstrating residues involved in the sulfation of tyrosines 276, 278, and 279 Blood, May 29, 2002; 99(12): 4422 - 4427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goto, N. Tamura, K. Eto, Y. Ikeda, and S. Handa Functional Significance of Adenosine 5'-Diphosphate Receptor (P2Y12) in Platelet Activation Initiated by Binding of von Willebrand Factor to Platelet GP Ib{alpha} Induced by Conditions of High Shear Rate Circulation, May 28, 2002; 105(21): 2531 - 2536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Douglas, K Michaelides, D A Gorog, E Durante-Mangoni, N Ahmed, G J Davies, and E G D Tuddenham Platelet membrane glycoprotein Ib{alpha} gene -5T/C Kozak sequence polymorphism as an independent risk factor for the occurrence of coronary thrombosis Heart, January 1, 2002; 87(1): 70 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Morigi, M. Galbusera, E. Binda, B. Imberti, S. Gastoldi, A. Remuzzi, C. Zoja, and G. Remuzzi Verotoxin-1-induced up-regulation of adhesive molecules renders microvascular endothelial cells thrombogenic at high shear stress Blood, September 15, 2001; 98(6): 1828 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsushita, D. Meyer, and J. E. Sadler Localization of von Willebrand Factor-binding Sites for Platelet Glycoprotein Ib and Botrocetin by Charged-to-Alanine Scanning Mutagenesis J. Biol. Chem., April 6, 2000; 275(15): 11044 - 11049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shen, G. M. Romo, J.-f. Dong, A. Schade, L. V. McIntire, D. Kenny, J. C. Whisstock, M. C. Berndt, J. A. Lopez, and R. K. Andrews Requirement of leucine-rich repeats of glycoprotein (GP) Ibalpha for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex Blood, February 1, 2000; 95(3): 903 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Kahn, T. G. Diacovo, D. F. Bainton, F. Lanza, J. Trejo, and S. R. Coughlin Glycoprotein V-Deficient Platelets Have Undiminished Thrombin Responsiveness and Do Not Exhibit a Bernard-Soulier Phenotype Blood, December 15, 1999; 94(12): 4112 - 4121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lepage, G. Uzan, N. Touche, M. Morales, J.-P. Cazenave, F. Lanza, and C. de la Salle Functional Characterization of the Human Platelet Glycoprotein V Gene Promoter: A Specific Marker of Late Megakaryocytic Differentiation Blood, November 15, 1999; 94(10): 3366 - 3380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ramakrishnan, P. S. Reeves, F. DeGuzman, U. Deshpande, K. Ministri-Madrid, R. B. DuBridge, and D. R. Phillips Increased thrombin responsiveness in platelets from mice lacking glycoprotein V PNAS, November 9, 1999; 96(23): 13336 - 13341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Savage, M. H. Ginsberg, and Z. M. Ruggeri Influence of Fibrillar Collagen Structure on the Mechanisms of Platelet Thrombus Formation Under Flow Blood, October 15, 1999; 94(8): 2704 - 2715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Marchese, E. Saldivar, J. Ware, and Z. M. Ruggeri Adhesive properties of the isolated amino-terminal domain of platelet glycoprotein Ibalpha in a flow field PNAS, July 6, 1999; 96(14): 7837 - 7842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. M. Ruggeri, J. A. Dent, and E. Saldivar Contribution of Distinct Adhesive Interactions to Platelet Aggregation in Flowing Blood Blood, July 1, 1999; 94(1): 172 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. De Candia, R. De Cristofaro, and R. Landolfi Thrombin-Induced Platelet Activation Is Inhibited by High- and Low-Molecular-Weight Heparin Circulation, June 29, 1999; 99(25): 3308 - 3314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miyata and Z. M. Ruggeri Distinct Structural Attributes Regulating von Willebrand Factor A1 Domain Interaction with Platelet Glycoprotein Ibalpha under Flow J. Biol. Chem., March 5, 1999; 274(10): 6586 - 6593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goto, H. Sakai, M. Goto, M. Ono, Y. Ikeda, S. Handa, and Z. M. Ruggeri Enhanced Shear-Induced Platelet Aggregation in Acute Myocardial Infarction Circulation, February 9, 1999; 99(5): 608 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mazzucato, P. Spessotto, A. Masotti, L. De Appollonia, M. R. Cozzi, A. Yoshioka, R. Perris, A. Colombatti, and L. De Marco Identification of Domains Responsible for von Willebrand Factor Type VI Collagen Interaction Mediating Platelet Adhesion under High Flow J. Biol. Chem., January 29, 1999; 274(5): 3033 - 3041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujita, Y. Hashimoto, S. Russell, B. Zieger, and J. Ware In Vivo Expression of Murine Platelet Glycoprotein Ibalpha Blood, July 15, 1998; 92(2): 488 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takafuta, G. Wu, G. F. Murphy, and S. S. Shapiro Human beta -Filamin Is a New Protein That Interacts with the Cytoplasmic Tail of Glycoprotein Ibalpha J. Biol. Chem., July 10, 1998; 273(28): 17531 - 17538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mazzucato, L. De Marco, A. Masotti, P. Pradella, W. F. Bahou, and Z. M. Ruggeri Characterization of the Initial alpha -Thrombin Interaction with Glycoprotein Ibalpha in Relation to Platelet Activation J. Biol. Chem., January 23, 1998; 273(4): 1880 - 1887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Asazuma, Y. Ozaki, K. Satoh, Y. Yatomi, M. Handa, Y. Fujimura, S. Miura, and S. Kume Glycoprotein Ib-von Willebrand Factor Interactions Activate Tyrosine Kinases in Human Platelets Blood, December 15, 1997; 90(12): 4789 - 4798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wu, D. W. Essex, F. J. Meloni, T. Takafuta, K. Fujimura, B. A. Konkle, and S. S. Shapiro Human Endothelial Cells in Culture and In Vivo Express on Their Surface All Four Components of the Glycoprotein Ib/IX/V Complex Blood, October 1, 1997; 90(7): 2660 - 2669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Moriki, M. Murata, T. Kitaguchi, H. Anbo, M. Handa, K. Watanabe, H. Takahashi, and Y. Ikeda Expression and Functional Characterization of an Abnormal Platelet Membrane Glycoprotein Ibalpha (Met239 right-arrow Val) Reported in Patients With Platelet-Type von Willebrand Disease Blood, July 15, 1997; 90(2): 698 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ravanat, M. Morales, D. O. Azorsa, S. Moog, S. Schuhler, P. Grunert, D. Loew, A. Van Dorsselaer, J.-P. Cazenave, and F. Lanza Gene Cloning of Rat and Mouse Platelet Glycoprotein V: Identification of Megakaryocyte-Specific Promoters and Demonstration of Functional Thrombin Cleavage Blood, May 1, 1997; 89(9): 3253 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miyata, S. Goto, A. B. Federici, J. Ware, and Z. M. Ruggeri Conformational Changes in the A1 Domain of von Willebrand Factor Modulating the Interaction with Platelet Glycoprotein Ibalpha J. Biol. Chem., April 12, 1996; 271(15): 9046 - 9053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goto, D. R. Salomon, Y. Ikeda, and Z. M. Ruggeri Characterization of the Unique Mechanism Mediating the Shear-dependent Binding of Soluble von Willebrand Factor to Platelets J. Biol. Chem., October 6, 1995; 270(40): 23352 - 23361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Pelletier, T. Kunicki, Z. M. Ruggeri, and V. Quaranta The Activation State of the Integrin [IMAGE][IMAGE][IMAGE][IMAGE] Affects Outside-in Signals Leading to Cell Spreading and Focal Adhesion Kinase Phosphorylation J. Biol. Chem., July 28, 1995; 270(30): 18133 - 18140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Meyer and J. E. B. Fox Interaction of Platelet Glycoprotein V with Glycoprotein Ib-IX Regulates Expression of the Glycoproteins and Binding of von Willebrand Factor to Glycoprotein Ib-IX in Transfected Cells J. Biol. Chem., June 16, 1995; 270(24): 14693 - 14699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsushita and J. E. Sadler Identification of Amino Acid Residues Essential for von Willebrand Factor Binding to Platelet Glycoprotein Ib J. Biol. Chem., June 2, 1995; 270(22): 13406 - 13414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Marchese, M. Murata, M. Mazzucato, P. Pradella, L. D. Marco, J. Ware, and Z. M. Ruggeri Identification of Three Tyrosine Residues of Glycoprotein Ib[IMAGE] with Distinct Roles in von Willebrand Factor and [IMAGE]-Thrombin Binding J. Biol. Chem., April 21, 1995; 270(16): 9571 - 9578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Yap, S. C. Hughan, S. L. Cranmer, W. S. Nesbitt, M. M. Rooney, S. Giuliano, S. Kulkarni, S. M. Dopheide, Y. Yuan, H. H. Salem, et al. Synergistic Adhesive Interactions and Signaling Mechanisms Operating between Platelet Glycoprotein Ib/IX and Integrin alpha IIbbeta 3. STUDIES IN HUMAN PLATELETS AND TRANSFECTED CHINESE HAMSTER OVARY CELLS J. Biol. Chem., December 22, 2000; 275(52): 41377 - 41388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Englund, R. J. Bodnar, Z. Li, Z. M. Ruggeri, and X. Du Regulation of von Willebrand Factor Binding to the Platelet Glycoprotein Ib-IX by a Membrane Skeleton-dependent Inside-out Signal J. Biol. Chem., May 11, 2001; 276(20): 16952 - 16959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. De Candia, S. W. Hall, S. Rutella, R. Landolfi, R. K. Andrews, and R. De Cristofaro Binding of Thrombin to Glycoprotein Ib Accelerates the Hydrolysis of Par-1 on Intact Platelets J. Biol. Chem., February 9, 2001; 276(7): 4692 - 4698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Soslau, R. Class, D. A. Morgan, C. Foster, S. T. Lord, P. Marchese, and Z. M. Ruggeri Unique Pathway of Thrombin-induced Platelet Aggregation Mediated by Glycoprotein Ib J. Biol. Chem., June 8, 2001; 276(24): 21173 - 21183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ware, S. Russell, and Z. M. Ruggeri Generation and rescue of a murine model of platelet dysfunction: The Bernard-Soulier syndrome PNAS, March 14, 2000; 97(6): 2803 - 2808. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |