HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Charo, I. F. Articles by Phillips, D. R. Search for Related Content PubMed PubMed Citation Articles by Charo, I. F. Articles by Phillips, D. R. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 21, 9935-9938, Jul, 1987

Platelet glycoprotein IIb-IIIa-like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix

IF Charo, LS Bekeart and DR Phillips

The adherence of human umbilical vein endothelial (HUVE) cells to adhesive matrix proteins was examined to determine if cell attachment and spreading were mediated by the glycoprotein (GP) IIb-IIIa complex on endothelial cells. The HUVE cells adhered well to glass slides that had been coated with fibronectin, vitronectin, fibrinogen, or von Willebrand factor but failed to adhere to albumin-coated or to uncoated slides. The HUVE cell attachment and spreading on vitronectin, fibrinogen, and von Willebrand factor were greatly inhibited by a GP IIb-IIIa monoclonal antibody (7E3). In contrast, HUVE cell attachment to fibronectin was not inhibited by 7E3 but was inhibited by a fibronectin-receptor antibody (alpha GP140), which had no effect on cell attachment to the other adhesive proteins. The 7E3 antibody, but not alpha GP140, disrupted HUVE cell monolayers by detaching cells from their naturally occurring extracellular matrix. These data indicate that platelet GP IIb-IIIa-like proteins mediate the adherence of HUVE cells to specific adhesive proteins and to the extracellular matrix.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Artoni, J. Li, B. Mitchell, J. Ruan, J. Takagi, T. A. Springer, D. L. French, and B. S. Coller Inaugural Article: Integrin {beta}3 regions controlling binding of murine mAb 7E3: Implications for the mechanism of integrin {alpha}IIb{beta}3 activation PNAS, September 7, 2004; 101(36): 13114 - 13120. [Abstract] [Full Text] [PDF]

				H. M. Nugent and E. R. Edelman Tissue Engineering Therapy for Cardiovascular Disease Circ. Res., May 30, 2003; 92(10): 1068 - 1078. [Abstract] [Full Text] [PDF]

				A. F. Drew, H. L. Schiman, K. W. Kombrinck, T. H. Bugge, J. L. Degen, and A. H. Kaufman Persistent Corneal Haze after Excimer Laser Photokeratectomy in Plasminogen-Deficient Mice Invest. Ophthalmol. Vis. Sci., January 1, 2000; 41(1): 67 - 72. [Abstract] [Full Text]

				S. H. Tam, P. M. Sassoli, R. E. Jordan, and M. T. Nakada Abciximab (ReoPro, Chimeric 7E3 Fab) Demonstrates Equivalent Affinity and Functional Blockade of Glycoprotein IIb/IIIa and {alpha}vß3 Integrins Circulation, September 15, 1998; 98(11): 1085 - 1091. [Abstract] [Full Text] [PDF]

				D. A. Beacham, L.-P. Tran, and S. S. Shapiro Cytokine Treatment of Endothelial Cells Increases Glycoprotein Ibalpha -Dependent Adhesion to von Willebrand Factor Blood, June 1, 1997; 89(11): 4071 - 4077. [Abstract] [Full Text] [PDF]

				K. Suehiro, J. Gailit, and E. F. Plow Fibrinogen Is a Ligand for Integrin alpha 5beta 1 on Endothelial Cells J. Biol. Chem., February 21, 1997; 272(8): 5360 - 5366. [Abstract] [Full Text] [PDF]

				S. E. D'Souza, V. J. Byers-Ward, E. E. Gardiner, H. Wang, and S.-S. Sung Identification of an Active Sequence within the First Immunoglobulin Domain of Intercellular Cell Adhesion Molecule-1 (ICAM-1) That Interacts with Fibrinogen J. Biol. Chem., September 27, 1996; 271(39): 24270 - 24277. [Abstract] [Full Text] [PDF]

				J. Lefkovits, E. F. Plow, and E. J. Topol Platelet Glycoprotein IIb/IIIa Receptors in Cardiovascular Medicine N. Engl. J. Med., June 8, 1995; 332(23): 1553 - 1559. [Full Text] [PDF]

				J. Marshall, D. Rutherford, A. McCartney, F Mitjans, S. Goodman, and I. Hart Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin J. Cell Sci., January 3, 1995; 108(3): 1227 - 1238. [Abstract] [PDF]

				M.H. Ginsberg, T.E. O'Toole, J.C. Loftus, and E.F. Plow Ligand Binding to Integrins: Dynamic Regulation and Common Mechanisms Cold Spring Harb Symp Quant Biol, January 1, 1992; 57(0): 221 - 231. [Abstract] [PDF]