HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suzuki, R. Articles by Morita, F. Search for Related Content PubMed PubMed Citation Articles by Suzuki, R. Articles by Morita, F. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 262, Issue 24, 11410-11412, 08, 1987

F-actin-binding synthetic heptapeptide having the amino acid sequence around the SH1 cysteinyl residue of myosin

R Suzuki, N Nishi, S Tokura and F Morita

The heptapeptide Ile-Arg-Ile-Cys-Arg-Lys-Gly-ethyl ester, having the amino acid sequence around the SH1 of myosin heavy chain, was coprecipitated with F-actin after ultracentrifugation. The heptapeptide inhibited the formation of acto-S-1 rigor complex by competing with S-1 for actin. Assuming a simple competitive inhibition, the dissociation constant of acto-heptapeptide complex was evaluated as 0.23 mM. An N- terminal tripeptide derivative from the heptapeptide Ile-Arg-Ile-methyl ester also formed a complex with F-actin with a dissociation constant of 1.1 mM. However, the other piece, Cys-Arg-Lys-Gly-ethyl ester, and a tetrapeptide, Val-Leu-Glu-Gly-ethyl ester, having the sequence adjacent to the N-terminal of the heptapeptide, scarcely bound with F-actin. These results suggest that part of the actin-binding site of myosin heavy chain around SH1 (Katoh, T., Katoh, H., and Morita, F. (1985) J. Biol. Chem. 260, 6723-6727) has the sequence of Ile-Arg-Ile and it is located adjacent to SH1 on its N-terminal side.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. Chen, K. Pavlish, and J. N. Benoit Myosin phosphorylation triggers actin polymerization in vascular smooth muscle Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2172 - H2177. [Abstract] [Full Text] [PDF]

				E.-K. Lim, M. R. Roberts, and D. J. Bowles Biochemical Characterization of Tomato Annexin p35. INDEPENDENCE OF CALCIUM BINDING AND PHOSPHATASE ACTIVITIES J. Biol. Chem., December 25, 1998; 273(52): 34920 - 34925. [Abstract] [Full Text] [PDF]

				J. R. Sims, S. Karp, and D. E. Ingber Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape J. Cell Sci., December 1, 1992; 103(4): 1215 - 1222. [Abstract] [PDF]