|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 263, Issue 34, 18078-18085, 12, 1988
MJ Kelley, AM Bailis, SA Henry and GM Carman
The addition of inositol to the growth medium of Saccharomyces cerevisiae
resulted in rapid changes in the rates of phospholipid biosynthesis. The
partitioning of the phospholipid intermediate CDP- diacylglycerol was
shifted to phosphatidylinositol at the expense of phosphatidylserine and
its derivatives phosphatidylethanolamine and phosphatidylcholine. Serine at
133-fold greater concentrations than that of inositol shifted the
partitioning of CDP-diacylglycerol to phosphatidylserine at the expense of
phosphatidylinositol but to a much lesser degree. Kinetic experiments with
pure phosphatidylserine synthase and phosphatidylinositol synthase
indicated that the partitioning of CDP-diacylglycerol between
phosphatidylserine and phosphatidylinositol was not governed by the
affinities both enzymes have for their common substrate CDP-diacylglycerol.
Instead, the main regulation of phosphatidylinositol and phosphatidylserine
synthesis was through the exogenous supply of inositol. The Km of inositol
(0.21 mM) for phosphatidylinositol synthase was 9-fold higher than
cytosolic concentration of inositol (24 microM). The Km of serine (0.83 mM)
for phosphatidylserine synthase was 3-fold below the cytosolic
concentration of serine (2.6 mM). Therefore, inositol supplementation
resulted in a dramatic increase in the rate of phosphatidylinositol
synthesis, whereas serine supplementation resulted in little affect on the
rate of phosphatidylserine synthesis. Inositol also contributed to the
regulation of phosphatidylinositol and phosphatidylserine synthesis by
having a direct affect on phosphatidylserine synthase activity. Kinetic
experiments with pure phosphatidylserine synthase showed that inositol was
a noncompetitive inhibitor of the enzyme with a Ki of 65 microM.
Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by inositol. Inositol is an inhibitor of phosphatidylserine synthase activity
Department of Food Science, Cook College, Rutgers University, New Brunswick, 08903.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. R. Nunez, S. A. Jesch, M. L. Gaspar, C. Almaguer, M. Villa-Garcia, M. Ruiz-Noriega, J. Patton-Vogt, and S. A. Henry Cell Wall Integrity MAPK Pathway Is Essential for Lipid Homeostasis J. Biol. Chem., December 5, 2008; 283(49): 34204 - 34217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gaspar, S. A. Jesch, R. Viswanatha, A. L. Antosh, W. J. Brown, S. D. Kohlwein, and S. A. Henry A Block in Endoplasmic Reticulum-to-Golgi Trafficking Inhibits Phospholipid Synthesis and Induces Neutral Lipid Accumulation J. Biol. Chem., September 12, 2008; 283(37): 25735 - 25751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Carman and S. A. Henry Phosphatidic Acid Plays a Central Role in the Transcriptional Regulation of Glycerophospholipid Synthesis in Saccharomyces cerevisiae J. Biol. Chem., December 28, 2007; 282(52): 37293 - 37297. [Full Text] [PDF]
|
|
|
|
|
|
G.-S. Han, S. Siniossoglou, and G. M. Carman The Cellular Functions of the Yeast Lipin Homolog Pah1p Are Dependent on Its Phosphatidate Phosphatase Activity J. Biol. Chem., December 21, 2007; 282(51): 37026 - 37035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Choi and G. M. Carman Respiratory Deficiency Mediates the Regulation of CHO1-encoded Phosphatidylserine Synthase by mRNA Stability in Saccharomyces cerevisiae J. Biol. Chem., October 26, 2007; 282(43): 31217 - 31227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Jesch, P. Liu, X. Zhao, M. T. Wells, and S. A. Henry Multiple Endoplasmic Reticulum-to-Nucleus Signaling Pathways Coordinate Phospholipid Metabolism with Gene Expression by Distinct Mechanisms J. Biol. Chem., August 18, 2006; 281(33): 24070 - 24083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gaspar, M. A. Aregullin, S. A. Jesch, and S. A. Henry Inositol Induces a Profound Alteration in the Pattern and Rate of Synthesis and Turnover of Membrane Lipids in Saccharomyces cerevisiae J. Biol. Chem., August 11, 2006; 281(32): 22773 - 22785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Han, G.-S. Han, W. M. Iwanyshyn, and G. M. Carman Regulation of the PIS1-encoded Phosphatidylinositol Synthase in Saccharomyces cerevisiae by Zinc J. Biol. Chem., August 12, 2005; 280(32): 29017 - 29024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Jesch, X. Zhao, M. T. Wells, and S. A. Henry Genome-wide Analysis Reveals Inositol, Not Choline, as the Major Effector of Ino2p-Ino4p and Unfolded Protein Response Target Gene Expression in Yeast J. Biol. Chem., March 11, 2005; 280(10): 9106 - 9118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Gardocki, M. Bakewell, D. Kamath, K. Robinson, K. Borovicka, and J. M. Lopes Genomic Analysis of PIS1 Gene Expression Eukaryot. Cell, March 1, 2005; 4(3): 604 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Kersting, H.-S. Choi, and G. M. Carman Regulation of the Yeast EKI1-encoded Ethanolamine Kinase by Inositol and Choline J. Biol. Chem., August 20, 2004; 279(34): 35353 - 35359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Iwanyshyn, G.-S. Han, and G. M. Carman Regulation of Phospholipid Synthesis in Saccharomyces cerevisiae by Zinc J. Biol. Chem., May 21, 2004; 279(21): 21976 - 21983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Choi, A. Sreenivas, G.-S. Han, and G. M. Carman Regulation of Phospholipid Synthesis in the Yeast cki1{Delta} eki1{Delta} Mutant Defective in the Kennedy Pathway: THE CHO1-ENCODED PHOSPHATIDYLSERINE SYNTHASE IS REGULATED BY mRNA STABILITY J. Biol. Chem., March 26, 2004; 279(13): 12081 - 12087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Gardocki and J. M. Lopes Expression of the Yeast PIS1 Gene Requires Multiple Regulatory Elements Including a Rox1p Binding Site J. Biol. Chem., October 3, 2003; 278(40): 38646 - 38652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhong and M. L. Greenberg Regulation of Phosphatidylglycerophosphate Synthase by Inositol in Saccharomyces cerevisiae Is Not at the Level of PGS1 mRNA Abundance J. Biol. Chem., September 5, 2003; 278(36): 33978 - 33984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-S. Park, D. J. O'Brien, and G. M. Carman Phosphorylation of CTP Synthetase on Ser36, Ser330, Ser354, and Ser454 Regulates the Levels of CTP and Phosphatidylcholine Synthesis in Saccharomyces cerevisiae J. Biol. Chem., May 30, 2003; 278(23): 20785 - 20794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Chang, E. W. Jones, and S. A. Henry Role of the Unfolded Protein Response Pathway in Regulation of INO1 and in the sec14 Bypass Mechanism in Saccharomyces cerevisiae Genetics, September 1, 2002; 162(1): 29 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Shirra, J. Patton-Vogt, A. Ulrich, O. Liuta-Tehlivets, S. D. Kohlwein, S. A. Henry, and K. M. Arndt Inhibition of Acetyl Coenzyme A Carboxylase Activity Restores Expression of the INO1 Gene in a snf1 Mutant Strain of Saccharomyces cerevisiae Mol. Cell. Biol., September 1, 2001; 21(17): 5710 - 5722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Birner, M. Bürgermeister, R. Schneiter, and G. Daum Roles of Phosphatidylethanolamine and of Its Several Biosynthetic Pathways in Saccharomyces cerevisiae Mol. Biol. Cell, April 1, 2001; 12(4): 997 - 1007. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Elkhaimi, M. R. Kaadige, D. Kamath, J. C. Jackson, H. Biliran Jr, and J. M. Lopes Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes Nucleic Acids Res., August 15, 2000; 28(16): 3160 - 3167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Patton-Vogt, P. Griac, A. Sreenivas, V. Bruno, S. Dowd, M. J. Swede, and S. A. Henry Role of the Yeast Phosphatidylinositol/Phosphatidylcholine Transfer Protein (Sec14p) in Phosphatidylcholine Turnover and INO1 Regulation J. Biol. Chem., August 15, 1997; 272(33): 20873 - 20883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shen and W. Dowhan Regulation of Phospholipid Biosynthetic Enzymes by the Level of CDP-Diacylglycerol Synthase Activity J. Biol. Chem., April 25, 1997; 272(17): 11215 - 11220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Carman and G. M. Zeimetz Regulation of Phospholipid Biosynthesis in the Yeast Saccharomyces cerevisiae J. Biol. Chem., June 7, 1996; 271(23): 13293 - 13296. [Full Text] [PDF]
|
|
|
|
|
|
H. Shen, P. N. Heacock, C. J. Clancey, and W. Dowhan The CDS1 Gene Encoding CDP-diacylglycerol Synthase In Saccharomyces cerevisiae Is Essential for Cell Growth J. Biol. Chem., January 12, 1996; 271(2): 789 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-I Wu, V. M. McDonough, J. T. NickelsJr., J. Ko, A. S. Fischl, T. R. Vales, A. H. MerrillJr., and G. M. Carman Regulation of Lipid Biosynthesis in Saccharomyces cerevisiae by Fumonisin B[IMAGE] J. Biol. Chem., June 2, 1995; 270(22): 13171 - 13178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lai, C. P. Bolognese, S. Swift, and P. McGraw Regulation of Inositol Transport in Saccharomyces cerevisiae Involves Inositol-induced Changes in Permease Stability and Endocytic Degradation in the Vacuole J. Biol. Chem., February 10, 1995; 270(6): 2525 - 2534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oshiro, S. Rangaswamy, X. Chen, G.-S. Han, J. E. Quinn, and G. M. Carman Regulation of the DPP1-encoded Diacylglycerol Pyrophosphate (DGPP) Phosphatase by Inositol and Growth Phase. INHIBITION OF DGPP PHOSPHATASE ACTIVITY BY CDP-DIACYLGLYCEROL AND ACTIVATION OF PHOSPHATIDYLSERINE SYNTHASE ACTIVITY BY DGPP J. Biol. Chem., December 22, 2000; 275(52): 40887 - 40896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Baudry, E. Swain, A. Rahier, M. Germann, A. Batta, S. Rondet, S. Mandala, K. Henry, G. S. Tint, T. Edlind, et al. The Effect of the erg26-1 Mutation on the Regulation of Lipid Metabolism in Saccharomyces cerevisiae J. Biol. Chem., April 13, 2001; 276(16): 12702 - 12711. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |