|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 266, Issue 12, 7774-7778, 04, 1991
JJ Cali and DW Russell
The enzyme sterol 27-hydroxylase catalyzes the first step in the oxidation
of the side chain of sterol intermediates in the bile acid synthesis
pathway. Human sterol 27-hydroxylase cDNAs were isolated from a liver cDNA
library by cross-hybridization with a previously cloned rabbit cDNA probe.
DNA sequence analysis of hybridization-positive clones predicted a human
sterol 27-hydroxylase consisting of a 33-amino- acid mitochondrial signal
sequence followed by a mature protein of 498 amino acids. RNA blotting
experiments demonstrated sterol 27- hydroxylase mRNAs of approximately 1.8
to 2.2 kilobases in liver and fibroblast cells. The steady state levels of
the mRNA did not change when cultured cells were grown in the presence or
absence of sterols. Introduction of the sterol 27-hydroxylase cDNA into
Simian COS cells resulted in the expression of active enzyme capable of
catalyzing multiple oxidation reactions (R-CH3----R-CH2OH----R-COOH) at
carbon 27 of sterol intermediates of the bile acid synthesis pathway.
Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. S Bevelander, E. S L C Pinto, A. V M Canario, T. Spanings, and G. Flik CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein J. Endocrinol., March 1, 2008; 196(3): 625 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. DuSell, M. Umetani, P. W. Shaul, D. J. Mangelsdorf, and D. P. McDonnell 27-Hydroxycholesterol Is an Endogenous Selective Estrogen Receptor Modulator Mol. Endocrinol., January 1, 2008; 22(1): 65 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fuda, N. B. Javitt, K. Mitamura, S. Ikegawa, and C. A. Strott Oxysterols are substrates for cholesterol sulfotransferase J. Lipid Res., June 1, 2007; 48(6): 1343 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Pikuleva CHOLESTEROL-METABOLIZING CYTOCHROMES P450 Drug Metab. Dispos., April 1, 2006; 34(4): 513 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. He, M. J. Cryle, J. J. O. De Voss, and P. R. de Montellano Calibration of the Channel That Determines the {omega}-Hydroxylation Regiospecificity of Cytochrome P4504A1: CATALYTIC OXIDATION OF 12-HALODODECANOIC ACIDS J. Biol. Chem., June 17, 2005; 280(24): 22697 - 22705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rahmaniyan, K. Patrick, and N. H. Bell Characterization of recombinant CYP2C11: a vitamin D 25-hydroxylase and 24-hydroxylase Am J Physiol Endocrinol Metab, April 1, 2005; 288(4): E753 - E760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Murtazina, U. Andersson, I.-S. Hahn, I. Bjorkhem, G. A. S. Ansari, and I. A. Pikuleva Phospholipids modify substrate binding and enzyme activity of human cytochrome P450 27A1 J. Lipid Res., December 1, 2004; 45(12): 2345 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Szanto, S. Benko, I. Szatmari, B. L. Balint, I. Furtos, R. Ruhl, S. Molnar, L. Csiba, R. Garuti, S. Calandra, et al. Transcriptional Regulation of Human CYP27 Integrates Retinoid, Peroxisome Proliferator-Activated Receptor, and Liver X Receptor Signaling in Macrophages Mol. Cell. Biol., September 15, 2004; 24(18): 8154 - 8166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Brown III, C. Theisler, S. Silberman, D. Magnuson, N. Gottardi-Littell, J. M. Lee, D. Yager, J. Crowley, K. Sambamurti, M. M. Rahman, et al. Differential Expression of Cholesterol Hydroxylases in Alzheimer's Disease J. Biol. Chem., August 13, 2004; 279(33): 34674 - 34681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Federico and M. T. Dotti Cerebrotendinous Xanthomatosis: Clinical Manifestations, Diagnostic Criteria, Pathogenesis, and Therapy J Child Neurol, September 1, 2003; 18(9): 633 - 638. [Abstract] [PDF]
|
|
|
|
|
|
M. Norlin, S. von Bahr, I. Bjorkhem, and K. Wikvall On the substrate specificity of human CYP27A1: implications for bile acid and cholestanol formation J. Lipid Res., August 1, 2003; 44(8): 1515 - 1522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Meir, D. Kitsberg, I. Alkalay, F. Szafer, H. Rosen, S. Shpitzen, L. B. Avi, B. Staels, C. Fievet, V. Meiner, et al. Human Sterol 27-Hydroxylase (CYP27) Overexpressor Transgenic Mouse Model. EVIDENCE AGAINST 27-HYDROXYCHOLESTEROL AS A CRITICAL REGULATOR OF CHOLESTEROL HOMEOSTASIS J. Biol. Chem., September 6, 2002; 277(37): 34036 - 34041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Javitt 25R,26-Hydroxycholesterol revisited: synthesis, metabolism, and biologic roles J. Lipid Res., May 1, 2002; 43(5): 665 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Davis, J. H. Miyake, T. Y. Hui, and N. J. Spann Regulation of cholesterol-7{alpha}-hydroxylase: BAREly missing a SHP J. Lipid Res., April 1, 2002; 43(4): 533 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Moore and R. A. Davis Expression of cholesterol-7{alpha}-hydroxylase in murine macrophages prevents cholesterol loading by acetyl-LDL J. Lipid Res., April 1, 2002; 43(4): 629 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Buhler, B. Witholt, B. Hauer, and A. Schmid Characterization and Application of Xylene Monooxygenase for Multistep Biocatalysis Appl. Envir. Microbiol., February 1, 2002; 68(2): 560 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bodin, L. Bretillon, Y. Aden, L. Bertilsson, U. Broome, C. Einarsson, and U. Diczfalusy Antiepileptic Drugs Increase Plasma Levels of 4beta -Hydroxycholesterolin Humans. EVIDENCE FOR INVOLVEMENT OF CYTOCHROME P450 3A4 J. Biol. Chem., October 12, 2001; 276(42): 38685 - 38689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Theodoropoulos, C. Demers, A. Mirshahi, and M. Gascon-Barre 1,25-Dihydroxyvitamin D3 downregulates the rat intestinal vitamin D3-25-hydroxylase CYP27A Am J Physiol Endocrinol Metab, August 1, 2001; 281(2): E315 - E325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Javitt, Y. C. Lee, C. Shimizu, H. Fuda, and C. A. Strott Cholesterol and Hydroxycholesterol Sulfotransferases: Identification, Distinction from Dehydroepiandrosterone Sulfotransferase, and Differential Tissue Expression Endocrinology, July 1, 2001; 142(7): 2978 - 2984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hall, P. Hylemon, Z. Vlahcevic, D. Mallonee, K. Valerie, N. Avadhani, and W. Pandak Overexpression of CYP27 in hepatic and extrahepatic cells: role in the regulation of cholesterol homeostasis Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G293 - G301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Lee, S. Hazard, J. D. Carpten, S. Yi, J. Cohen, G. T. Gerhardt, G. Salen, and S. B. Patel Fine-mapping, mutation analyses, and structural mapping of cerebrotendinous xanthomatosis in U.S. pedigrees J. Lipid Res., February 1, 2001; 42(2): 159 - 169. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Verrips, L. H. Hoefsloot, G. C. H. Steenbergen, J. P. Theelen, R. A. Wevers, F. J. M. Gabreels, B. G. M. van Engelen, and L. P. W. J. van den Heuvel Clinical and molecular genetic characteristics of patients with cerebrotendinous xanthomatosis Brain, May 1, 2000; 123(5): 908 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verrips, B. G. M. van Engelen, R. A. Wevers, B. M. van Geel, J. R. M. Cruysberg, L. P. W. J. van den Heuvel, A. Keyser, and F. J. M. Gabreels Presence of Diarrhea and Absence of Tendon Xanthomas in Patients With Cerebrotendinous Xanthomatosis Arch Neurol, April 1, 2000; 57(4): 520 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Honda, G. Salen, S. Shefer, Y. Matsuzaki, G. Xu, A. K. Batta, G. S. Tint, and N. Tanaka Regulation of 25- and 27-hydroxylation side chain cleavage pathways for cholic acid biosynthesis in humans, rabbits, and mice: assay of enzyme activities by high-resolution gas chromatography;-mass spectrometry J. Lipid Res., March 1, 2000; 41(3): 442 - 451. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. J. Schroepfer Jr. Oxysterols: Modulators of Cholesterol Metabolism and Other Processes Physiol Rev, January 1, 2000; 80(1): 361 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Shiga, R Fukuyama, S Kimura, K Nakajima, and S Fushiki Mutation of the sterol 27-hydroxylase gene (CYP27) results in truncation of mRNA expressed in leucocytes in a Japanese family with cerebrotendinous xanthomatosis J. Neurol. Neurosurg. Psychiatry, November 1, 1999; 67(5): 675 - 677. [Abstract] [Full Text]
|
|
|
|
|
|
Q.-s. Zhu, P. von Dippe, W. Xing, and D. Levy Membrane Topology and Cell Surface Targeting of Microsomal Epoxide Hydrolase. EVIDENCE FOR MULTIPLE TOPOLOGICAL ORIENTATIONS J. Biol. Chem., September 24, 1999; 274(39): 27898 - 27904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Bell-Parikh and F. P. Guengerich Kinetics of Cytochrome P450 2E1-Catalyzed Oxidation of Ethanol to Acetic Acid via Acetaldehyde J. Biol. Chem., August 20, 1999; 274(34): 23833 - 23840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Honda, G. Salen, S. Shefer, A. K. Batta, M. Honda, G. Xu, G. S. Tint, Y. Matsuzaki, J. Shoda, and N. Tanaka Bile acid synthesis in the Smith-Lemli-Opitz syndrome: effects of dehydrocholesterols on cholesterol 7{alpha}-hydroxylase and 27-hydroxylase activities in rat liver J. Lipid Res., August 1, 1999; 40(8): 1520 - 1528. [Abstract] [Full Text]
|
|
|
|
|
|
N. Wakamatsu, M. Hayashi, H. Kawai, H. Kondo, Y. Gotoda, Y. Nishida, R. Kondo, S. Tsuji, and T. Matsumoto Mutations producing premature termination of translation and an amino acid substitution in the sterol 27-hydroxylase gene cause cerebrotendinous xanthomatosis associated with parkinsonism J. Neurol. Neurosurg. Psychiatry, August 1, 1999; 67(2): 195 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Verrips, G. J. Lycklama a Nijeholt, F. Barkhof, B. G. M. Van Engelen, P. Wesseling, J. A. F. M. Luyten, R. A. Wevers, J. Stam, J. H. J. Wokke, L. P. W. J. van den Heuvel, et al. Spinal xanthomatosis: a variant of cerebrotendinous xanthomatosis Brain, August 1, 1999; 122(8): 1589 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. JONES, S. A. STRUGNELL, and H. F. DeLUCA Current Understanding of the Molecular Actions of Vitamin D Physiol Rev, October 1, 1998; 78(4): 1193 - 1231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Pikuleva, A. Babiker, M. R. Waterman, and I. Bjorkhem Activities of Recombinant Human Cytochrome P450c27 (CYP27) Which Produce Intermediates of Alternative Bile Acid Biosynthetic Pathways J. Biol. Chem., July 17, 1998; 273(29): 18153 - 18160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Rosen, A. Reshef, N. Maeda, A. Lippoldt, S. Shpizen, L. Triger, G. Eggertsen, I. Bjorkhem, and E. Leitersdorf Markedly Reduced Bile Acid Synthesis but Maintained Levels of Cholesterol and Vitamin D Metabolites in Mice with Disrupted Sterol 27-Hydroxylase Gene J. Biol. Chem., June 12, 1998; 273(24): 14805 - 14812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chen, S. Kubota, and Y. Seyama Alternative pre-mRNA splicing of the sterol 27-hydroxylase gene (CYP 27) caused by a G to A mutation at the last nucleotide of exon 6 in a patient with cerebrotendinous xanthomatosis (CTX) J. Lipid Res., March 1, 1998; 39(3): 509 - 517. [Abstract] [Full Text]
|
|
|
|
|
|
G. K. Fu, D. Lin, M. Y. H. Zhang, D. D. Bikle, C. H. L. Shackleton, W. L. Miller, and A. A. Portale Cloning of Human 25-Hydroxyvitamin D-1{alpha}-Hydroxylase and Mutations Causing Vitamin D-Dependent Rickets Type 1 Mol. Endocrinol., December 1, 1997; 11(13): 1961 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Lala, P. M. Syka, S. B. Lazarchik, D. J. Mangelsdorf, K. L. Parker, and R. A. Heyman Activation of the orphan nuclear receptor steroidogenic factor 1 by oxysterols PNAS, May 13, 1997; 94(10): 4895 - 4900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Shayiq and R. M. Shayiq Role of Gene Overlap in the Regulation of mRNA Translation for Mitochondrial Cytochrome P-450c27/25 in the Rat J. Biol. Chem., February 14, 1997; 272(7): 4050 - 4057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Eggertsen, M. Olin, U. Andersson, H. Ishida, S. Kubota, U. Hellman, K.-I. Okuda, and I. Bjorkhem Molecular Cloning and Expression of Rabbit Sterol 12alpha -Hydroxylase J. Biol. Chem., December 13, 1996; 271(50): 32269 - 32275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. von Dippe, M. Amoui, R. H. Stellwagen, and D. Levy The Functional Expression of Sodium-dependent Bile Acid Transport in Madin-Darby Canine Kidney Cells Transfected with the cDNA for Microsomal Epoxide Hydrolase J. Biol. Chem., July 26, 1996; 271(30): 18176 - 18180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Payne, C. Shackleton, H. Toms, I. Ben-Shlomo, S. Kol, M. deMoura, J. F. Strauss, and E. Y. Adashi A Novel Nonhepatic Hydroxycholesterol 7alpha-Hydroxylase That Is Markedly Stimulated by Interleukin-1beta J. Biol. Chem., August 11, 1995; 270(32): 18888 - 18896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Memon, A. H. Moser, J. K. Shigenaga, C. Grunfeld, and K. R. Feingold In Vivo and in Vitro Regulation of Sterol 27-Hydroxylase in the Liver during the Acute Phase Response. POTENTIAL ROLE OF HEPATOCYTE NUCLEAR FACTOR-1 J. Biol. Chem., August 3, 2001; 276(32): 30118 - 30126. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |