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J Biol Chem, Vol. 273, Issue 30, 19055-19059, July 24, 1998

Isolation of a Delta 5-Fatty Acid Desaturase Gene from Mortierella alpina

Louise V. MichaelsonDagger , Colin M. LazarusDagger , Gareth Griffiths, Johnathan A. Napierparallel , and A. Keith StobartDagger

From the Dagger  School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG,  Horticulture Research International, Wellesbourne, Warwick CV35 9EF, and parallel  Institute of Arable Crops Research-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, United Kingdom

Arachidonic acid (C20:4 Delta 5,8,11,14) is a polyunsaturated fatty acid synthesized by the Delta 5-fatty acid desaturation of di-homo-gamma -linolenic acid (C20:3 Delta 8,11,14). In mammals, it is known to be a precursor of the prostaglandins and the leukotrienes but it is also accumulated by the filamentous fungus Mortierella alpina. We have isolated a cDNA encoding the Delta 5-fatty acid desaturase from M. alpina via a polymerase chain reaction-based strategy using primers designed to the conserved histidine box regions of microsomal desaturases, and confirmed its function by expression in the yeast Saccharomyces cerevisiae. Analysis of the lipids from the transformed yeast demonstrated the accumulation of arachidonic acid. The M. alpina Delta 5-desaturase is the first example of a cloned Delta 5-desaturase, and differs from other fungal desaturases previously characterized by the presence of an N-terminal domain related to cytochrome b5.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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