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J. Biol. Chem., Vol. 277, Issue 49, 46849-46851, December 6, 2002
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From the International Centre for Genetic Engineering and
Biotechnology (ICGEB), P. O. Box 10504, Aruna Asaf Ali Marg, New
Delhi 110 067, India
Insecticidal crystal proteins of Bacillus
thuringiensis bind to receptors in the midgut of susceptible
insects leading to pore formation and death of the insect. The identity
of the receptor is not clearly established. Recently a direct
interaction between a cloned and heterologously expressed
aminopeptidase (slapn) from Spodoptera litura
and the Cry1C protein was demonstrated by immunofluorescence and
in vitro ligand blot interaction. Here we show that
administration of slapn double-stranded RNA to S. litura larvae reduces its expression. As a consequence of the
reduced expression, a corresponding decrease in the sensitivity of
these larvae to Cry1C toxin was observed. The gene silencing was
retained during the insect's moulting and development and transmitted
to the subsequent generation albeit with a reduced effect. These
results directly implicate larval midgut aminopeptidase N as receptor
for Bacillus thuringiensis insecticidal proteins.
ACCELERATED PUBLICATION
Silencing of Midgut Aminopeptidase N of Spodoptera
litura by Double-stranded RNA Establishes Its Role as
Bacillus thuringiensis Toxin Receptor*
,,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
These authors contributed equally to this work.
§
To whom correspondence should be addressed: Insect Resistance
Group, ICGEB, P. O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110 067, India. Tel.: 91-11-6195007 (or 91-11-6177357, 91-11-6189358, 91-11-6189360, or 91-11-6189361); Fax: 91-11-616-2316; E-mail: raj@icgeb.res.in.
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