| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print June 19, 2000
University of California, Irvine, Irvine, CA 92697-3900
Corresponding Author: poulos{at}uci.edu
The structure of the first P450 identified in archaea, CYP119 from Sulfolobus solfataricus, has been solved in 2 different crystal forms. The first form has imidazole and the second has 4-phenylimidazole coordinated to the heme iron. A comparison of the 2 structures reveals an unprecedented rearrangement of the active site in order to adapt to the different size and shape of ligands bound to the heme iron. These changes involve unraveling of the F helix C-terminal segment in order to extend a loop structure connecting the F and G helices, allowing the longer loop to dip down into the active site and interact with the smaller imidazole ligand. A comparison of CYP119 with P450cam and P450eryF indicates an extensive clustering of aromatic residues may be the structural basis for the enhanced thermal stability of CYP119. An additional feature of the 4-phenylimidazole bound structure is a zinc ion tetrahedrally bound by symmetry related His and Glu residues.
J. Biol. Chem, 10.1074/jbc.M004281200
Submitted on May 18, 2000
Revised on June 15, 2000
Accepted on June 15, 2000
Crystal Structure of a Thermophilic Cytochrome P450 from the Archaeon Sulfolobus solfataricus
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Li, Z. Chang, Z. Pan, Z.-Q. Fu, and X. Wang Modes of heme binding and substrate access for cytochrome P450 CYP74A revealed by crystal structures of allene oxide synthase PNAS, September 16, 2008; 105(37): 13883 - 13888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Makino, H. Sugimoto, Y. Shiro, S. Asamizu, H. Onaka, and S. Nagano Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeleton PNAS, July 10, 2007; 104(28): 11591 - 11596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verras, A. Alian, and P. R.O. d. Montellano Cytochrome P450 active site plasticity: attenuation of imidazole binding in cytochrome P450cam by an L244A mutation Protein Eng. Des. Sel., November 1, 2006; 19(11): 491 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Sherman, S. Li, L. V. Yermalitskaya, Y. Kim, J. A. Smith, M. R. Waterman, and L. M. Podust The Structural Basis for Substrate Anchoring, Active Site Selectivity, and Product Formation by P450 PikC from Streptomyces venezuelae J. Biol. Chem., September 8, 2006; 281(36): 26289 - 26297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baudry, S. Rupasinghe, and M. A. Schuler Class-dependent sequence alignment strategy improves the structural and functional modeling of P450s Protein Eng. Des. Sel., August 1, 2006; 19(8): 345 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Muralidhara, S. Negi, C. C. Chin, W. Braun, and J. R. Halpert Conformational Flexibility of Mammalian Cytochrome P450 2B4 in Binding Imidazole Inhibitors with Different Ring Chemistry and Side Chains: SOLUTION THERMODYNAMICS AND MOLECULAR MODELING J. Biol. Chem., March 24, 2006; 281(12): 8051 - 8061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Poulos STRUCTURAL AND FUNCTIONAL DIVERSITY IN HEME MONOOXYGENASES Drug Metab. Dispos., January 1, 2005; 33(1): 10 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Scott, M. A. White, Y. A. He, E. F. Johnson, C. D. Stout, and J. R. Halpert Structure of Mammalian Cytochrome P450 2B4 Complexed with 4-(4-Chlorophenyl)imidazole at 1.9-A Resolution: INSIGHT INTO THE RANGE OF P450 CONFORMATIONS AND THE COORDINATION OF REDOX PARTNER BINDING J. Biol. Chem., June 25, 2004; 279(26): 27294 - 27301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Schoch, J. K. Yano, M. R. Wester, K. J. Griffin, C. D. Stout, and E. F. Johnson Structure of Human Microsomal Cytochrome P450 2C8: EVIDENCE FOR A PERIPHERAL FATTY ACID BINDING SITE J. Biol. Chem., March 5, 2004; 279(10): 9497 - 9503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Podust, H. Bach, Y. Kim, D. C. Lamb, M. Arase, D. H. Sherman, S. L. Kelly, and M. R. Waterman Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways Protein Sci., January 1, 2004; 13(1): 255 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Poulos Cytochrome P450 flexibility PNAS, November 11, 2003; 100(23): 13121 - 13122. [Full Text] [PDF]
|
|
|
|
|
|
S. Nagano, H. Li, H. Shimizu, C. Nishida, H. Ogura, P. R. O. de Montellano, and T. L. Poulos Crystal Structures of Epothilone D-bound, Epothilone B-bound, and Substrate-free Forms of Cytochrome P450epoK J. Biol. Chem., November 7, 2003; 278(45): 44886 - 44893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Podust, Y. Kim, M. Arase, B. A. Neely, B. J. Beck, H. Bach, D. H. Sherman, D. C. Lamb, S. L. Kelly, and M. R. Waterman The 1.92-A Structure of Streptomyces coelicolor A3(2) CYP154C1. A NEW MONOOXYGENASE THAT FUNCTIONALIZES MACROLIDE RING SYSTEMS J. Biol. Chem., March 28, 2003; 278(14): 12214 - 12221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-S. Lee, A. Yamada, H. Sugimoto, I. Matsunaga, H. Ogura, K. Ichihara, S.-i. Adachi, S.-Y. Park, and Y. Shiro Substrate Recognition and Molecular Mechanism of Fatty Acid Hydroxylation by Cytochrome P450 from Bacillus subtilis. CRYSTALLOGRAPHIC, SPECTROSCOPIC, AND MUTATIONAL STUDIES J. Biol. Chem., March 7, 2003; 278(11): 9761 - 9767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Leys, C. G. Mowat, K. J. McLean, A. Richmond, S. K. Chapman, M. D. Walkinshaw, and A. W. Munro Atomic Structure of Mycobacterium tuberculosis CYP121 to 1.06 A Reveals Novel Features of Cytochrome P450 J. Biol. Chem., February 7, 2003; 278(7): 5141 - 5147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Yano, F. Blasco, H. Li, R. D. Schmid, A. Henne, and T. L. Poulos Preliminary Characterization and Crystal Structure of a Thermostable Cytochrome P450 from Thermus thermophilus J. Biol. Chem., January 3, 2003; 278(1): 608 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zerbe, O. Pylypenko, F. Vitali, W. Zhang, S. Rouset, M. Heck, J. W. Vrijbloed, D. Bischoff, B. Bister, R. D. Sussmuth, et al. Crystal Structure of OxyB, a Cytochrome P450 Implicated in an Oxidative Phenol Coupling Reaction during Vancomycin Biosynthesis J. Biol. Chem., November 27, 2002; 277(49): 47476 - 47485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Panek and M. R. O'Brian A whole genome view of prokaryotic haem biosynthesis Microbiology, August 1, 2002; 148(8): 2273 - 2282. [Full Text] [PDF]
|
|
|
|
|
|
P. J. Winn, S. K. Ludemann, R. Gauges, V. Lounnas, and R. C. Wade Comparison of the dynamics of substrate access channels in three cytochrome P450s reveals different opening mechanisms and a novel functional role for a buried arginine PNAS, April 16, 2002; 99(8): 5361 - 5366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Dunn, I. J. Dmochowski, A. M. Bilwes, H. B. Gray, and B. R. Crane Probing the open state of cytochrome P450cam with ruthenium-linker substrates PNAS, October 12, 2001; (2001) 221297998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Podust, T. L. Poulos, and M. R. Waterman Crystal structure of cytochrome P450 14alpha -sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors PNAS, March 13, 2001; 98(6): 3068 - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Maves and S. G. Sligar Understanding thermostability in cytochrome P450 by combinatorial mutagenesis Protein Sci., January 1, 2001; 10(1): 161 - 168. [Abstract] [Full Text]
|
|
|
|
|
|
K. Nakayama, A. Puchkaev, and I. A. Pikuleva Membrane Binding and Substrate Access Merge in Cytochrome P450 7A1, a Key Enzyme in Degradation of Cholesterol J. Biol. Chem., August 10, 2001; 276(33): 31459 - 31465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamazaki, T. Shimada, M. V. Martin, and F. P. Guengerich Stimulation of Cytochrome P450 Reactions by Apo-cytochrome b5. EVIDENCE AGAINST TRANSFER OF HEME FROM CYTOCHROME P450 3A4 TO APO-CYTOCHROME b5 OR HEME OXYGENASE J. Biol. Chem., August 10, 2001; 276(33): 30885 - 30891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Dunn, I. J. Dmochowski, A. M. Bilwes, H. B. Gray, and B. R. Crane Probing the open state of cytochrome P450cam with ruthenium-linker substrates PNAS, October 23, 2001; 98(22): 12420 - 12425. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
