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Fig. 1.
The experimental system for single molecule
observation of redox control. The
3
3
TCT complex was
immobilized onto a slide glass via histidine tags, and microspheres
(blue) were attached to a cysteine residue (light
blue) on the subunit with a biotin/streptavidin linkage
(gray). The switch region containing two Cys residues is
shown as a red line. The disulfide bond in this region is
reduced by DTT plus thioredoxin (Red) and reformed by
oxidation with CuCl2 (Ox), thus modulating the
activity. HS, sulfhydryl group.
