HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 28, 18909-18913, July 14, 2006

This Article Abstract Full Text (PDF) All Versions of this Article: 281/28/18909    most recent C600042200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Crack, J. C. Articles by Thomson, A. J. Search for Related Content PubMed PubMed Citation Articles by Crack, J. C. Articles by Thomson, A. J. Social Bookmarking                      What's this?

Detection of Sulfide Release from the Oxygen-sensing [4Fe-4S] Cluster of FNR^*

Jason C. Crack¹, Jeffrey Green, Nick E. Le Brun, and Andrew J. Thomson²

From the Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom and the Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom

Received for publication, February 22, 2006 , and in revised form, May 4, 2006.

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

 
The Escherichia coli FNR protein regulates the transcription of >100 genes in response to environmental O₂, thereby coordinating the response to anoxia. Under O₂-limiting conditions, FNR binds a [4Fe-4S]²⁺ cluster through four cysteine residues (Cys²⁰, Cys²³, Cys²⁹, Cys¹²²). The acquisition of the [4Fe-4S]²⁺ cluster converts FNR into the transcriptionally active dimeric form. Upon exposure to O₂, the cluster converts to a [2Fe-2S]²⁺ form, generating FNR monomers that no longer bind DNA with high affinity. The mechanism of the cluster conversion reaction and the nature of the released iron and sulfur are of considerable current interest. Here, we report the application of a novel in vitro method, involving 5,5'-dithiobis-(2-nitrobenzoic acid), for determining the oxidation state of the sulfur atoms released during FNR cluster conversion following the addition of O₂. Conversion of [4Fe-4S]²⁺ to [2Fe-2S]²⁺ clusters by O₂ for both native and reconstituted FNR results in the release of 2 sulfide ions per [4Fe-4S]²⁺ cluster. This demonstrates that the reaction between O₂ and the [4Fe-4S]²⁺ cluster does not require sulfide oxidation and hence must entail iron oxidation.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

 
Escherichia coli is a facultative anaerobe that adopts different metabolic modes in a hierarchal manner in response to the availability of oxygen (1–3). This hierarchy reflects the amount of energy that can be harnessed in each metabolic mode and is maintained by transcriptional regulators that monitor either environmental oxygen or the cellular redox state (4). Aerobic respiration, a process that utilizes molecular oxygen (O₂) as the terminal electron acceptor, is preferred over anaerobic respiration, which utilizes alternative electron acceptors such as nitrate because of the enormous energetic benefits conferred by aerobic respiration (3). Likewise, anaerobic respiration is preferred over fermentation for similar reasons (3).

The global transcriptional regulator FNR (designated due to defects in the utilization of fumarate or nitrate during anaerobic growth in corresponding fnr mutants (5)) activates the expression of genes that encode components of alternative electron transport chains essential for anaerobic respiration (6, 7). Under anaerobic growth conditions, FNR also represses the expression of some genes associated with aerobic respiration (6, 8, 9).

FNR shares sequence homology with the cyclic-AMP receptor protein (CRP)³ (10) and, like CRP, consists of two distinct domains that provide DNA binding and sensory functions (11). The C-terminal DNA-binding domain recognizes specific FNR binding sequences within FNR-controlled promoters. The N-terminal sensory domain contains five cysteine residues, four of which (Cys²⁰, Cys²³, Cys²⁹, Cys¹²²) are essential (12) and assumed to be capable of binding either a [4Fe-4S]²⁺ or a [2Fe-2S]²⁺ cluster (13, 14). A variety of studies have shown that FNR is specifically activated under anaerobic conditions by the acquisition of one [4Fe-4S]²⁺ cluster per monomer (15–17). This promotes dimerization and enhances site specific DNA-binding to target promoters (18, 19). Exposure of the [4Fe-4S]²⁺ cluster to oxygen or air causes it to undergo a conversion into a [2Fe-2S]²⁺ cluster, both in vivo and in vitro, resulting in the loss of site specific DNA binding (13, 19–22).

Recently we reported that the [4Fe-4S]²⁺ to [2Fe-2S]²⁺ cluster conversion proceeds through a [3Fe-4S]⁺ intermediate, leading to production of hydrogen peroxide presumably by a two electron reduction of oxygen and the release of two iron ions (17). The oxidation states of the released iron atoms have yet to be unequivocally established. An alternative [4Fe-4S]²⁺ to [2Fe-2S]²⁺ conversion mechanism was recently proposed by Sutton et al. (23). Using Ferene, a strong Fe²⁺ chelator, release of two Fe²⁺ ions per cluster was determined, leading to the suggestion that oxidation of released sulfide ions to S⁰, rather than cluster iron, was taking place. Here we report, using a novel analytical method, direct determination of the amount of cluster sulfide ions released upon in vitro conversion of [4Fe-4S] FNR into [2Fe-2S] FNR. The data demonstrate that cluster sulfur is ejected as sulfide ions and is not, therefore, oxidized upon cluster conversion. The implications of this for the mechanism of oxygen sensing by FNR [4Fe-4S]²⁺ clusters are discussed.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

 
Purification of [4Fe-4S] FNR—Native FNR protein was over-produced in aerobic cultures of JRG5369 (E. coli BL21DE3 pGS1859). Plasmid pGS1859 was constructed by ligating the fnr gene from pGS199 (24) as a NcoI-BamHI fragment into the corresponding restriction sites within the expression vector pET11d (Stratagene). FNR overproduction was initiated by the addition of isopropyl -D-thiogalactopyranoside. Formation of [4Fe-4S] FNR was promoted, in vivo, by sparging cultures with oxygen-free nitrogen gas at 4 °C, essentially as described by Sutton and Kiley (25). All protein purification and handling was carried out under strictly anaerobic conditions in an anaerobic cabinet (Belle Technology), typically operating at 2.0 ppm O₂ by volume, equipped with a specially designed fridge-freezer for anaerobic sample storage and fitted with a liquid nitrogen access port. All buffers were sparged with oxygen-free nitrogen gas for a minimum of 2 h. The purity of isolated FNR was assessed by SDS-PAGE. Full details of the growth conditions and purification of FNR will be published elsewhere.

Purification of Reconstituted [4Fe-4S] FNR—Glutathione S-transferase-FNR fusion protein was produced in aerobically grown E. coli BL21DE3 pGS572, as described previously (17). FNR was cleaved from the fusion protein using thrombin and [4Fe-4S] FNR reconstituted in vitro, as described previously (17), except that a 1-ml HiTrap heparin column (GE Healthcare) was used (25) in place of a Sephadex G25 column to remove low molecular weight contaminants and to perform buffer exchange.

Purification of [2Fe-2S] FNR—An aliquot of native [4Fe-4S] FNR (1 ml, 119 µM in cluster) in buffer A was removed from the anaerobic cabinet, treated with 500 µl of buffer A containing dissolved atmospheric oxygen, and gently agitated in air for 90 s. The sample was then returned to the anaerobic cabinet. [2Fe-2S] FNR was isolated from cluster breakdown products using a Sephadex G25 column (PD10, GE Healthcare). The protein, iron, and sulfide contents of the sample were determined as described below.

Quantitative Methods—FNR protein concentrations were determined using the method of Bradford (Bio-Rad), with bovine serum albumin as the standard (26) and a previously determined correction factor of 0.83 (15). FNR iron content was determined in the following way: 0.1 ml of 21.7% HNO₃ was added to the same volume of protein and incubated at 95 °C for 30 min. Cooled samples were centrifuged to remove any precipitate, treated with 0.6 ml of 7.5% (w/v) ammonium acetate, 0.1 ml of 12.5% (w/v) ascorbic acid, 0.1 ml of 10 mM Ferene, mixed, and incubated at room temperature for 30 min before absorbance at 593 nm was measured. Iron concentrations were determined by reference to a calibration curve generated from Fe³⁺ solutions in the range 0–200 µM, prepared from SpectrosoL standard iron solution (BDH, Lot OC495679), and treated as described above. Acid-labile sulfide was determined according to the method of Beinert (27). Based on the analyses, both native and reconstituted [4Fe-4S]²⁺ FNR samples exhibited _{405 nm} values of 16,220 ± 135 M^–1 cm^–1, in close agreement to previously reported values (23). The concentration of dissolved atmospheric oxygen present in buffer solutions was determined by chemical analysis according to the method of Winkler (28).

Quantitation of Sulfide Released during Cluster Conversion—Quantitation of sulfide released from the cluster during the [4Fe-4S]²⁺ to [2Fe-2S]²⁺ conversion was carried out using a modified version of the procedure based on DTNB (Ellman's reagent) reported by Nashef and colleagues (29). Briefly, [4Fe-4S] FNR (5–10 µM) was treated with DTNB (200 µM) under anaerobic conditions and incubated at room temperature for 2 min prior to measurement of absorbance at 412 nm (30). The [4Fe-4S]²⁺ to [2Fe-2S]²⁺ cluster transition was subsequently induced by injecting an aliquot of buffer containing dissolved atmospheric oxygen (40 µM final concentration). Absorbance at 412 nm was measured again after an incubation period of 12 min at room temperature (sufficient time for the reaction (A_{412 nm}) to plateau under the given conditions (data not shown)). The reactive thiol content of [2Fe-2S] FNR was measured as absorbance changes at 412 nm following the anaerobic addition of DTNB (200 µM) and incubation for 2 min at room temperature.

Reactive thiol and free sulfide concentrations were calculated by determining the concentration of released TNB anion, using an ₄₁₂ value of 14,151 M^–1 cm^–1 in buffer A at pH 6.8, 12,344 M^–1 cm^–1 in buffer B at pH 7.5, or 14,611 M^–1 cm^–1 in buffer B containing 6 M guanidine HCl (see below). A values at 412 nm were corrected for the changes at 412 nm, which were due only to the [4Fe-4S]²⁺ to [2Fe-2S]²⁺ conversion. To determine whether the liberation of the TNB anions was due, in part, to the generation of superoxide or hydrogen peroxide, experiments were repeated in the presence of catalase (268 units) and superoxide dismutase (35 units). Iron released during the conversion was determined by adding Ferene (100 µM) and ascorbate (600 µM), to generate [Fe(II)(Ferene)₃]^4–, and by incubating at room temperature for 2 min prior to measurement of absorbance at 593 nm. Adventitiously bound iron was determined by treating an anaerobic [4Fe-4S] FNR sample with Ferene and ascorbate as described above. Experiments were performed in two different buffer systems: 10 mM potassium phosphate, 400 mM KCl, 10% (v/v) glycerol, pH 6.8 (buffer A), and 25 mM HEPES, 2.5 mM CaCl₂, 100 mM NaCl, 100 mM NaNO₃, pH 7.5 (buffer B).

We verified that the [4Fe-4S]²⁺ cluster was stable to the presence of DTNB and Ferene in both buffer systems tested under anaerobic conditions, at least for the duration of the experiment (data not shown). The response of DTNB to sulfide was calibrated using a standard solution of Na₂S prepared as described by Beinert (27) and verified by iodometric titration as described by Vogel (28). 0.1 ml of Na₂S was added to 2 ml of buffer A containing excess DTNB (2.4 mM) and absorbance at 412 nm measured after 2 min. The amount of TNB anion produced was calculated using a ₄₁₂ value of 14,151 M^–1 cm^–1. To assess the effects of protein and Fe²⁺, DTNB reactions were repeated in the presence of lysozyme (0.8 mg/ml) and (NH₄)₂Fe(SO₄)₂ (117 µM). Cysteine, rather than Na₂S, was used for the Fe²⁺ control experiment due to the poor solubility of FeS. All determinations were carried out in triplicate. Absorbance measurements were made with a Jasco V550 UV-visible spectrophotometer.

	RESULTS AND DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

 
Since the initial report by Ellman (31) on the thiol-specific reactions of DTNB, the reagent has been used extensively as a means for the quantitation of thiol groups in proteins. However, DTNB may also be used as a colorimetric reagent, as reported by Nashef et al. (29), for the determination of sulfide ions, see Scheme 1. We have suitably modified the published method (29) to quantify sulfide released from FNR during [4Fe-4S]²⁺ to [2Fe-2S]²⁺ cluster conversion.

View larger version (7K): [in this window] [in a new window]   SCHEME 1

The addition of excess DTNB to [4Fe-4S] FNR under anaerobic conditions causes spectral changes due to the release of TNB anions (Fig. 1A). These arise from the reaction of DTNB with free protein thiol. FNR contains five cysteine residues of which four (Cys²⁰, Cys²³, Cys²⁹, Cys¹²²) ligate the [4Fe-4S] cluster. Hence Cys¹⁶ should be available for reaction with a modifying reagent such as DTNB, see Scheme 2. In addition, samples of reconstituted and native FNR are not 100% replete with [4Fe-4S] cluster. There is a component of cluster-free (apo-) protein typically ranging from 15 to 30% and 23 to 43% for reconstituted and native FNR, respectively. All five cysteines of apo-FNR are potentially available for reaction with DTNB. However, it has been demonstrated recently (32) that only four out of the five cysteines present in apo-FNR are reactive toward thiol-specific modifying reagents under anaerobic conditions in the presence of denaturants. We have found that the apo-FNR content of samples contains up to three thiols available for modification (see Table 1) depending upon the method used for purification. Under anaerobic denaturing conditions (buffer B containing 6 M guanidine HCl) we have found 4.7 (±0.4) and 4.1 (±0.5) thiols available for modification per monomer in reconstituted and native FNR, respectively (data not shown), consistent with the observations of Achebach and colleagues (32).

To determine the amount of sulfide ion released during FNR cluster conversion, a correction to the A_{412 nm} value was made to account for the spectral changes arising from the cluster conversion [4Fe-4S] to [2Fe-2S] (Fig. 1B).

The [2Fe-2S] clusters are believed to have the same cysteine ligands as the [4Fe-4S]²⁺ clusters (13). To confirm that cluster conversion does not expose any of the [4Fe-4S]²⁺ cysteine ligands to reaction with thiol modification reagents, [2Fe-2S] FNR was isolated, as described under "Experimental Procedures." Reaction of an aliquot of 5.2 µM [2Fe-2S] FNR (containing 5.2 µM protein, 10.9 µM iron, and 10.2 µM sulfide) with DTNB resulted in a TNB anion concentration of 5.7 µM, indicating the modification of 1.1 (±0.1) thiols per [2Fe-2S] FNR. In the absence of apo-FNR, this is consistent with the modification of Cys¹⁶ in each [2Fe-2S] FNR molecule. This supports the conclusion that Cys²⁰, Cys²³, Cys²⁹, and Cys¹²² are the ligands that bind both the [4Fe-4S]²⁺ and [2Fe-2S]²⁺ clusters (13). Hence the observed increase in absorbance at 412 nm is not due to further thiol modification. Instead, we ascribe it to the reaction of DTNB with sulfide ions released during cluster conversion.

View larger version (15K): [in this window] [in a new window]   FIGURE 1. Quantitation of sulfide released during cluster conversion. A, UV-visible absorbance spectra of: 8.0 µM reconstituted FNR containing 5.6 mM [4Fe-4S]2+ in buffer A (see "Expeimental Procedures") before (solid black line) and after the addition of DTNB (184 µM final concentration) (solid green line). The arrow indicates A at 412 nm, which corresponds to the free thiol content of the sample; B, the sample in A (dashed black and green lines) following the addition of oxygen containing buffer (final O2 concentration 40 mM)(solid green line). The spectrum in solid black results from an equivalent addition of oxygen to 8.0 mM reconstituted FNR containing 5.6 µM [4Fe-4S]2+ in buffer A in the absence of DTNB. From these two spectra, the A412 nm due to sulfide released during the [4Fe-4S] to [2Fe-2S] switch can be calculated (as indicated by arrows); C, the DTNB-containing sample in B following the addition of Ferene and ascorbate (91.1 and 617 µM, respectively) (solid blue line). The arrow indicates A593 nm corresponds to the recovery of iron (13.6µM) released during the [4Fe-4S] to [2Fe-2S] switch. The sample contained 1.8 µM adventitious iron, resulting in 11.8 µM released iron. All absorbance changes corresponds to reconstituted sample 3 in Table 1.

The [4Fe-4S]²⁺ cluster formally consists of two Fe²⁺ and two Fe³⁺ ions that are valence delocalised providing an average oxidation state of Fe^2.5+ across all four iron ions. The [2Fe-2S]²⁺ cluster formally contains two Fe³⁺ ions, therefore ejection of two Fe²⁺ ions with two sulfide ions from the [4Fe-4S]²⁺ cluster with formation of a [2Fe-2S]²⁺ cluster is not oxidative (see Equation 1).

(Eq. 1)

If one Fe²⁺ ion was oxidized to Fe³⁺ by oxygen (here called metal oxidation) to yield a [2Fe-2S]²⁺ cluster then a superoxide ion, one Fe³⁺ ion and one Fe²⁺ ion would be expected as the products (see Equation 2).

(Eq. 2)

Indeed, such a mixture of iron oxidation states has been observed by Khoroshilova et al. (13, 16). Furthermore, we cannot rule out the possibility that the hydrogen peroxide detected following reaction of [4Fe-4S] FNR with oxygen (17) originates from the dismutation of superoxide. Importantly, it has been reported that superoxide can reduce DTNB to TNB species (33). Therefore, the sulfide release experiments were repeated in the presence of catalase and superoxide dismutase. No specific effects attributable to superoxide (or hydrogen peroxide) were detected (Table 1).

View larger version (14K): [in this window] [in a new window]   SCHEME 2

(Eq. 3)

Previously we have proposed a mechanism for FNR cluster conversion based upon the observation of a [3Fe-4S]¹⁺ intermediate cluster together with the detection of hydrogen peroxide generated during cluster conversion (Ref. 17; see Equation 4). Note that the [3Fe-4S]¹⁺ cluster contains three Fe³⁺ ions.

(Eq. 4)

This mechanism results in an overall two electron oxidation reaction, in which both Fe²⁺ ions present in the [4Fe-4S] cluster are oxidized to Fe³⁺ ions (see Equation 5).

(Eq. 5)

View larger version (17K): [in this window] [in a new window]   SCHEME 3

In conclusion, our results demonstrate that two sulfide ions are ejected from the FNR [4Fe-4S]²⁺ cluster in response to oxygen, in vitro, irrespective of whether the [4Fe-4S] FNR is reconstituted or native protein. This is consistent with the idea that the reaction between oxygen and the cluster occurs via metal oxidation, rather than via a sulfide oxidation pathway. The method of sulfide ion determination reported here might be more widely applicable to other studies of iron-sulfur systems that involve cluster conversion or disassembly.

	FOOTNOTES

 
^* This work was supported by Biotechnology and Biological Sciences Research Council Grant BB/C500360/1 and by a Wellcome Trust award from the Joint Infrastructure Fund for equipment. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence may be addressed. Tel.: 44-1603-593830; Fax: 44-1603-592003; E-mail: J.Crack{at}uea.ac.uk. ² To whom correspondence may be addressed. Tel.: 44-1603-593051; Fax: 44-1603-593045; E-mail: A.Thomson{at}uea.ac.uk.

³ The abbreviations used are: CRP, cyclic-AMP receptor protein; DTNB, 5,5'-dithiobis-(2-nitrobenzoic acid); Ferene, 5,5'(3-(2-pyridyl)-1,2,4-triazine-5,6-diyl)-bis-2-furansulfonate; TNB, 5-thio-2-nitrobenzoate.

	ACKNOWLEDGMENTS

 
We are grateful to Prof. Dennis Dean (Virginia Tech) for plasmid pDB551 and to Belle Technology for their assistance with the anaerobic glove box fridge-freezer.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

 

1. Guest, J. R. (1995) Philos. Trans. R. Soc. Lond. B. Biol. Sci. 350, 189–202[Medline] [Order article via Infotrieve]
2. Spiro, S., and Guest, J. R. (1991) Trends Biochem. Sci. 16, 310–314[CrossRef][Medline] [Order article via Infotrieve]
3. Unden, G., and Bongaerts, J. (1997) Biochim. Biophys. Acta 1320, 217–234[Medline] [Order article via Infotrieve]
4. Perrenoud, A., and Sauer, U. (2005) J. Bacteriol. 187, 3171–3179[Abstract/Free Full Text]
5. Lambden, P. R., and Guest, J. R. (1976) J. Gen. Microbiol. 97, 145–160[Medline] [Order article via Infotrieve]
6. Kang, Y. S., Weber, K. D., Yu, Q., Kiley, P. J., and Blattner, F. R. (2005) J. Bacteriol. 187, 1135–1160[Abstract/Free Full Text]
7. Unden, G., and Trageser, M. (1991) Antonie Van Leeuwenhoek 59, 65–76[CrossRef][Medline] [Order article via Infotrieve]
8. Shalel-Levanon, S., San, K. Y., and Bennett, G. N. (2005) Metab. Eng. 7, 364–374[CrossRef][Medline] [Order article via Infotrieve]
9. Shalel-Levanon, S., San, K. Y., and Bennett, G. N. (2005) Biotech. Bioeng. 92, 147–159
10. Shaw, D. J., Rice, D. W., and Guest, J. R. (1983) J. Mol. Biol. 166, 241–247[Medline] [Order article via Infotrieve]
11. Green, J., Scott, C., and Guest, J. R. (2001) Adv. Microb. Physiol. 44, 1–34[Medline] [Order article via Infotrieve]
12. Green, J., Sharrocks, A. D., Green, B., Geisow, M., and Guest, J. R. (1993) Mol. Microbiol. 8, 61–68[CrossRef][Medline] [Order article via Infotrieve]
13. Khoroshilova, N., Popescu, C., Munck, E., Beinert, H., and Kiley, P. J. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 6087–6092[Abstract/Free Full Text]
14. Kiley, P. J., and Beinert, H. (1999) FEMS Microbiol. Rev. 22, 341–352[CrossRef]
15. Green, J., Bennett, B., Jordan, P., Ralph, E. T., Thomson, A. J., and Guest, J. R. (1996) Biochem. J. 316, 887–892
16. Khoroshilova, N., Beinert, H., and Kiley, P. J. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 2499–2503[Abstract/Free Full Text]
17. Crack, J., Green, J., and Thomson, A. J. (2004) J. Biol. Chem. 279, 9278–9286[Abstract/Free Full Text]
18. Sharrocks, A. D., Green, J., and Guest, J. R. (1991) Proc. Biol. Sci. 245, 219–226
19. Lazazzera, B. A., Bates, D. M., and Kiley, P. J. (1993) Genes Dev. 7, 1993–2005[Abstract/Free Full Text]
20. Lazazzera, B. A., Beinert, H., Khoroshilova, N., Kennedy, M. C., and Kiley, P. J. (1996) J. Biol. Chem. 271, 2762–2768[Abstract/Free Full Text]
21. Popescu, C. V., Bates, D. M., Beinert, H., Munck, E., and Kiley, P. J. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 13431–13435[Abstract/Free Full Text]
22. Jordan, P. A., Thomson, A. J., Ralph, E. T., Guest, J. R., and Green, J. (1997) FEBS Lett. 416, 349–352[CrossRef][Medline] [Order article via Infotrieve]
23. Sutton, V. R., Mettert, E. L., Beinert, H., and Kiley, P. J. (2004) J. Bacteriol. 186, 8018–8025[Abstract/Free Full Text]
24. Spiro, S., and Guest, J. R. (1987) Microbiology 133, 3279–3288[Medline] [Order article via Infotrieve]
25. Sutton, V. R., and Kiley, P. J. (2003) Methods Enzymol. 370, 300–312[Medline] [Order article via Infotrieve]
26. Bradford, M. M. (1976) Anal. Biochem. 72, 248–254[CrossRef][Medline] [Order article via Infotrieve]
27. Beinert, H. (1983) Anal. Biochem. 131, 373–378[CrossRef][Medline] [Order article via Infotrieve]
28. Vogel, A. I. (1989) in Textbook of Quantitative Chemical Analysis (Mendham, J., Jeffery, G. H., Bassett, J., and Denney, R. C., eds) 5th Ed., pp. 395–399, Longman Scientific & Technical, Harlow, UK
29. Nashef, A. S., Osuga, D. T., and Feeney, R. E. (1977) Anal. Biochem. 79, 394–405[Medline] [Order article via Infotrieve]
30. Riddles, P. W., Blakeley, R. L., and Zerner, B. (1983) Methods Enzymol. 91, 49–60[Medline] [Order article via Infotrieve]
31. Ellman, G., and Lysko, H. (1979) Anal. Biochem. 93, 98–102[Medline] [Order article via Infotrieve]
32. Achebach, S., Selmer, T., and Unden, G. (2005) FEBS J. 272, 4260–4269[CrossRef][Medline] [Order article via Infotrieve]
33. Peterson, D. A., Archer, S. L., and Weir, E. K. (1994) Biochem. Biophys. Res. Commun. 200, 1586–1591[Medline] [Order article via Infotrieve]

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. A. Gaskell, J. C. Crack, G. H. Kelemen, M. I. Hutchings, and N. E. Le Brun RsmA Is an Anti-sigma Factor That Modulates Its Activity through a [2Fe-2S] Cluster Cofactor J. Biol. Chem., October 26, 2007; 282(43): 31812 - 31820. [Abstract] [Full Text] [PDF]

				J. D. Partridge, R. K. Poole, and J. Green The Escherichia coli yhjA gene, encoding a predicted cytochrome c peroxidase, is regulated by FNR and OxyR Microbiology, May 1, 2007; 153(5): 1499 - 1509. [Abstract] [Full Text] [PDF]

				A. J. Jervis and J. Green In Vivo Demonstration of FNR Dimers in Response to Lower O2 Availability J. Bacteriol., April 1, 2007; 189(7): 2930 - 2932. [Abstract] [Full Text] [PDF]

				S. T. Pullan, M. D. Gidley, R. A. Jones, J. Barrett, T. M. Stevanin, R. C. Read, J. Green, and R. K. Poole Nitric Oxide in Chemostat-Cultured Escherichia coli Is Sensed by Fnr and Other Global Regulators: Unaltered Methionine Biosynthesis Indicates Lack of S Nitrosation J. Bacteriol., March 1, 2007; 189(5): 1845 - 1855. [Abstract] [Full Text] [PDF]

				J. C. Crack, J. Green, M. R. Cheesman, N. E. Le Brun, and A. J. Thomson Superoxide-mediated amplification of the oxygen-induced switch from [4Fe-4S] to [2Fe-2S] clusters in the transcriptional regulator FNR PNAS, February 13, 2007; 104(7): 2092 - 2097. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 281/28/18909    most recent C600042200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Crack, J. C. Articles by Thomson, A. J. Search for Related Content PubMed PubMed Citation Articles by Crack, J. C. Articles by Thomson, A. J. Social Bookmarking                      What's this?