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J. Biol. Chem., Vol. 281, Issue 43, 99933, October 27, 2006

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A New Role for DNA Polymerase

Epigenetic gene silencing can result from modifications in gene expression due to changes in DNA methylation or chromatin structure. In Saccharomyces cerevisiae, DNA polymerase (Pol ) has been shown to bind to double-stranded DNA (dsDNA) and to participate in the stable inheritance of the silenced state of chromatin. Pol is a four-subunit complex that contains a catalytic subunit, Pol2p, and three auxiliary subunits, Dpb2p, Dpb3p, and Dpb4p. Dpb3p and Dpb4p contain a histone-fold motif, and cells lacking DPB3 and/or DPB4 are defective in silencing, suggesting the subunits' involvement in dsDNA binding.

DNA polymerase binds to double-stranded DNA.

In this Paper of the Week, Toshiaki Tsubota and colleagues show that neither Pol2p-Dpb2p nor Dpb3p-Dpb4p binds stably to dsDNA but that binding is efficiently reconstituted when the two subassemblies are combined, indicating that both complexes are required for stable association with dsDNA. Further characterization of mutant forms of Pol , having amino acid substitutions in Dpb3p, suggests that the Dpb3p-Dpb4p subassembly in Pol binds dsDNA around its dimeric histone-fold structure in a manner resembling the histone-DNA interaction. The Pol mutants also displayed reduced telomeric silencing, suggesting that the dsDNA binding property of Pol is required for epigenetic silencing at telomeres.

FOOTNOTES

See referenced article, J. Biol. Chem. 2006, 281, 32898-32908

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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Related Collections Papers Of The Week Social Bookmarking                      What's this?