Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.R600023200 on September 20, 2006

J. Biol. Chem., Vol. 281, Issue 48, 36477-36481, December 1, 2006
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/48/36477    most recent
R600023200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bundschu, K.
Right arrow Articles by Schuh, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bundschu, K.
Right arrow Articles by Schuh, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Minireview

The VASP-Spred-Sprouty Domain Puzzle*

Karin Bundschu{ddagger}§1, Ulrich Walter§, and Kai Schuh§

From the {ddagger}Abteilung Biochemie und Molekulare Biologie, Universität Ulm, 89081 Ulm, §Institut für Klinische Biochemie und Pathobiochemie, Universität Würzburg, 97080 Würzburg, and Physiologisches Institut, Universität Würzburg, 97070 Würzburg, Germany


   ABSTRACT
TOP
 ABSTRACT
Family Members, Structure, and...
 Domains: Structure and Functions
 Signaling Pathways and...
 Summary and Future Perspectives
 REFERENCES
 
Sprouty-related proteins with an EVH1 domain (Spreds) belong to a new protein family harboring a conserved N-terminal EVH1 domain, which is related to the VASP (vasodilator-stimulated phosphoprotein) EVH1 domain (Enabled/VASP homology 1 domain) and a C-terminal Sprouty-related domain, typical for Sprouty proteins. Spreds were, like Sproutys, initially discovered as inhibitors of the Ras/MAPK pathway, and the SPR (Sprouty-related) domains of both protein families seem to be very important for many protein interactions and cellular processes. VASP was initially characterized as a proline-rich substrate of protein kinases A and G in human platelets and later shown to be a scaffold protein, regulating both signal transduction pathways and the actin filament system. The VASP-EVH1 domain is known to bind specifically to a FP4 binding motif, which is, for example, present in the focal adhesion proteins vinculin and zyxin. In this review we give a structural and functional overview on these three protein families and ask whether nature plays a modular protein domain puzzle with stable exchangeable elements or if these closely related domains have various functions when pasted in a different protein context.


   Family Members, Structure, and Expression
TOP
 ABSTRACT
 Family Members, Structure, and...
Domains: Structure and Functions
 Signaling Pathways and...
 Summary and Future Perspectives
 REFERENCES
 
Spred—Spred2 proteins are a new family of membrane-associated suppressors of growth factor-induced ERK activation. The Drosophila founder member AE33 (1) was followed by human, mouse, and Xenopus Spreds (2, 3) and, recently, by Eve-3, a splice variant of Spred-3 (4). Spred family members contain an N-terminal EVH1 domain, a central c-Kit binding domain (KBD), and a C-terminal Sprouty-like cysteine-rich domain (SPR domain) (2, 5). Spred-3 lacks a functional c-Kit binding domain (2), and Eve-3 consists of merely a single EVH1 domain (4). So far, four binding partners were described, namely Raf1, caveolin-1, Ras, and RhoA (5–8). The former two bind to the Spred-SPR domain, but for the latter two no binding sites are known so far. Spred-1 is expressed predominantly in adult brain (9) and in some fetal tissues, suggesting a role during development (9). Spred-2 expression was rare in fetal tissues but mostly ubiquitous in adults, whereas an especially strong expression was seen in neural tissues and different glandular epithelia (9, 10). Spred-3 was detected only in brain (2, 9), whereas Eve-3 was limited to the developed liver (4).

VASP—Ena/VASP (vasodilator-stimulated phosphoprotein) proteins contain, like Spreds, an N-terminal EVH1 domain. Mammalian VASP was first characterized in platelets as a proline-rich substrate of cAMP- and cGMP-dependent protein kinases (11). Additional members of the Ena/VASP family are the Drosophila Enabled (Ena), its mammalian homologue Mena (mammalian Enabled), the C. elegans homologue unc34, and the murine Evl (Enabled/vasodilator-stimulated phosphoprotein-like) (12–14). In general, the EVH1 domain is followed by a central proline-rich region responsible for interaction with Src homology 3 domains and profilins (compiled in Ref. 15) and, finally, by an EVH2 domain, mediating binding to G- and F-actin (16–18). Because of their important role in actin cytoskeleton organization, members of the Ena/VASP family are expressed most likely in almost all mammalian cell types (11, 19). VASP is associated to actin filaments and localized in focal adhesion complexes, dynamic membrane regions, leading membrane areas of non-epithelial cells, and cell/cell contacts of Madin-Darby canine kidney cells (12). Drosophila Enabled, Mena, and unc34 are localized in axonal structures and are important during neuronal development (13, 20).

Sprouty—Sprouty proteins contain, like Spreds, a highly conserved cysteine-rich domain at the C terminus (21–25) and are inhibitors of the MAPK signaling pathway. The N-terminal regions of Sprouty proteins are astonishingly variable, but they contain a conserved SH2 domain binding motif (NXYXXXP) including a central tyrosine residue (Tyr-55 in Sprouty2) (26, 27). So far, Drosophila Sprouty, Xenopus Sprouty, avian Sprouty (chick Sprouty2), and four mammalian isoforms have been identified (21–25, 28). Interaction partners of Sproutys are c-Cbl (cellular homologue of Casitas B lineage lymphoma proto-oncogene product), Grb2 (growth factor receptor-bound 2), Gap1 (GTPase-activating protein), FRS2 (fibroblast growth factor receptor substrate 2), SHP-2 (Src homology 2 domain-containing protein-tyrosine phosphatase 2), Raf1, TESK1 (testis-specific protein kinase-1), caveolin-1, and PTP1B (protein-tyrosine phosphatase 1B) (for review see Refs. 29 and 30), whereas the latter four bind to the C-terminal part. Binding to caveolin-1 targets Sprouty, like Spred, to membrane microdomains (31), TESK1 binding suppresses cell spreading by inhibiting the kinase activity of TESK1 (32), and PTP1 binding inhibits cell migration (33). Murine Sprouty1, -2, and -4 are widely expressed in embryonic and adult tissues (22–24), whereas Sprouty3 expression is restricted to adult brain and testis (24, 34). In unstimulated cells, Sprouty proteins are distributed evenly in the cytoplasm, except of human Sprouty2, which is additionally colocalized with microtubules. Following ligand-dependent RTK activation, a subpopulation translocates to the leading edge of the plasma membrane (31, 35–37).


   Domains: Structure and Functions
TOP
 ABSTRACT
 Family Members, Structure, and...
 Domains: Structure and Functions
Signaling Pathways and...
 Summary and Future Perspectives
 REFERENCES
 
EVH1—So far, four EVH1 families are known, the Ena/VASP, the Wiskott-Aldrich-Syndrome (WASP), the Homer/Vesl, and the Spred family. The EVH1 domain specifically binds proteins with proline-rich sequences (38–41), thereby playing an important role in the integrity of the cytoskeleton, actin-based cell motility, postsynaptical signal transduction, cell proliferation, and differentiation (reviewed in Refs. 15 and 42).

The EVH1 domain of the Ena/VASP family recognizes the distinct (E/D)FPPPPX(D/E) (FP4) binding motif (40, 43). This motif is regarded as a protein binding site, present in the focal adhesion proteins vinculin (39, 40) and zyxin (40), the axon guidance protein roundabout (Robo) (44), lipoma preferred partner (LPP) (45), semaphorin 6A-1 (Sema6A-1) (43), T-cell signaling Fyn-binding protein/SLP-76-associated protein (Fyb/SLAP) (46), and the surface protein ActA of Listeria (40).

In Spred proteins, the N-terminal EVH1 domain seems to play an essential role in the inhibition of ERK activation (4, 5, 47). Recently, structural information on the Spred EVH1 domain revealed that this domain adopts, like the other EVH1 domains, a pleckstrin homology fold (48, 49). However, for the Spred EVH1 domain a different induced fit peptide binding mechanism has been proposed even in the absence of a characterized ligand for this domain (48, 49), which makes the identification of binding partners a point of major interest (Fig. 1).

KBD—KBD in the middle part of the Spred proteins consists of about 50 amino acids (amino acids 234–286) and is not related to any previously identified tyrosine kinase interaction domains (5). Spred-3 with a non-functional KBD and deletion mutants of Spred-1 in this region revealed that this internal region is involved but not essential for ERK suppression (2, 5, 50). This raises the question of what the physiological function of this domain might be.

SPR—Spred and Sprouty proteins harbor a well conserved cysteine-rich SPR domain at the C terminus. In the case of Spred-2, it is required for efficient suppression of stem cell factor-induced ERK phosphorylation and suppression of hematopoietic cell development (51). A C-terminal deletion mutant of Spred-1 was shown to act as a dominant negative form and augments growth factor-induced ERK activation (5, 50). Contrary data were recently published by King et al. (47), where {Delta}C-Spred-2 was unable to suppress ERK activation but {Delta}C-Spred-1 and Eve-3 were still functional in suppressing ERK activation (4, 47). Therefore, future work has to be done, elucidating the physiological function of the SPR domain.

The SPR domain of Spred and Sprouty proteins is thought to be palmitoylated and necessary for membrane localization after growth factor stimulation (2, 5, 31, 37, 52, 53). Additionally, the minimal membrane-targeting region of the SPR domain in both protein families is necessary and sufficient for plasma membrane localization and binding to phosphatidylinositol 4,5-bisphosphate (37). Moreover, mutations disrupting this region interfere with their functions in modulating cell migration, cell proliferation, and ERK/MAPK activation (52, 53). All four mammalian Sprouty proteins are able to form hetero- and homo-oligomers via their C-terminal SPR domain (54), and although each of the Sprouty isoforms by itself inhibits growth factor-induced activation of the ERK pathway, hetero-oligomers show a more pronounced inhibitory activity (54). Recently, Spred-1 and Spred-2 were also presented to form heterodimers via their SPR domains, which increases the variety of regulatory possibilities (47).


Figure 1
View larger version (40K):
[in this window]
[in a new window]

  		FIGURE 1.
Domain functions and binding partners of VASP, Spred, and Sprouty. Numbers refer to reference numbers. RBM, Raf binding motif.

 

   Signaling Pathways and Physiological Functions
TOP
 ABSTRACT
 Family Members, Structure, and...
 Domains: Structure and Functions
 Signaling Pathways and...
Summary and Future Perspectives
 REFERENCES
 
Spred—Spreds were first described as suppressors of the Ras/Raf/MAPK signaling pathway (5). They were constitutively associated with Ras, but the inhibitory effect was exerted by suppressing the phosphorylation and activation of Raf (5). King et al. (47) located the target upstream of Ras, suggesting different interaction modes dependent on the cellular context. Spreds are potent inhibitors of a wide range of mitogenic stimuli like different growth factors, cytokines, and chemokines, but the suppressing effect seems to be restricted to the Ras/ERK/MAPK pathway (2, 7, 47, 50, 55).

In vivo studies using knock-out mouse models begin now to uncover the physiological Spred functions. Spred-1–/– and Spred-2–/– mice revealed that these proteins are not existentially necessary for fertility and development, and young adult mice are viable and show no apparent abnormalities (56, 57). However, loss of functional Spred-2 protein causes a dwarf phenotype, similar to hypochondroplasia, a common form of human dwarfism (56). In this context, Spred-2 seems to be an important modulator of bone morphogenesis by inhibiting the fibroblast growth factor-induced MAPK pathway (56). A similar phenotype was also mentioned for Spred-1–/– mice (57), underlining the role of Spred proteins in growth regulation and supporting the idea of overlapping physiological functions. Furthermore, Spreds seem to be important for hematopoiesis. In Spred-2–/– midgestation mouse embryos, Spred-2 suppresses hematopoietic processes by inhibiting MAPK activation (51), and stem cell factor or interleukin 3 stimulation of mature Spred-1–/– bone marrow-derived mast cells resulted in increased cell proliferation and MAPK activation (50). Therefore, Spred-2 could serve as a negative regulator of embryonic (51) and Spred-1 of mature late phase hematopoiesis, respectively (50). Moreover, Spred-1 seems to play a role in the regulation of eosinophils during allergic asthma processes (57). A specific function of Spred-3, which is expressed exclusively in brain (2), is not known yet.

VASP—Members of the Ena/VASP family are regarded as regulators of the actin cytoskeleton. Ena/VASP proteins promote actin polymerization and assembly, and several mechanisms have been proposed, which include the regulation of nucleation, bundling, branching, and, perhaps somewhat controversial, capping. However, the precise mechanisms remain to be elucidated and may also vary with the cell type and model used (15, 58, 59). At the molecular level, VASP proteins are considered as some kind of adapters, linking the proteins binding to the EVH1 domain to the actin polymerization process, which is regulated by the EVH2 domain or vice versa, guiding the actin polymerization machinery to intracellular spaces predetermined by EVH1-interacting proteins (13, 17, 40, 45, 60, 61).

Phosphorylation by protein kinase A and G at the site within the VASP EVH2 domain (Ser-239) but not at Ser-153/157 is established to inhibit F-actin binding/bundling by VASP (62, 63), which may also be caused by AMP-dependent kinase-mediated phosphorylation of VASP threonine 278.3 Moreover, the functions of VASP-like proteins can be adjusted to the particular cellular needs by protein kinase C phosphorylation (Ser-157) (64) or by several phosphatases (65). A further level of actin cytoskeleton regulation was added by the observation that SH3 domains interact with the central proline-rich region (for review see Ref. 15), located between the two EVH domains, thereby influencing a variety of pathways. The profilin/G-actin complexes are also recruited by this central region (66), thereby bringing these complexes in close proximity to the nascent filamentous actin bundle emerging from the flower bouquet-like tetramer of VASP-like proteins, held together by the tetramerization domains at the C termini of EVH2 domains (16, 67).

In vertebrates, genetic analysis of Ena/VASP function was hampered by the broad and overlapping expression of the three highly related family members Mena, VASP, and EVL (Ena-VASP-like). Mice deficient in either Mena or VASP exhibit subtle defects in forebrain commissure formation and platelet activation, respectively (68–71). Mena–/–/VASP–/– double mutants die perinatally and display defects in neurulation, in development of craniofacial structures, and in the formation of several fiber tracts in the central and peripheral nervous system (72). It is likely that the expression of the third family member, EVL, masks the requirement for Ena/VASP function in other cell types of Mena–/–/VASP–/– animals.

Sprouty—Like Spreds, members of the Sprouty family negatively regulate ERK activation (21, 73, 74). However, the related Sprouty proteins seem to differ in their pathway specificity and regulate other signaling pathways in addition to ERK (28, 31). Sprouty can target RTK signaling at many levels, upstream of ERK/MAPK and downstream of RTKs, and the exact position of Sprouty in the pathway is probably dependent on the cellular context (for review see Ref. 30). Compared with Spreds, mammalian Sproutys are more selective inhibitors, blocking fibroblast growth factor- but not epidermal growth factor-induced ERK activation (31, 55, 75, 76). Moreover, Sprouty family members regulate the RTK signaling not only negatively but also positively (reviewed in Refs. 77 and 78). This effect might be due to the large differences in the N-terminal areas of these proteins, but the molecular mechanisms need further clarification.

Sproutys act as antagonists in many physiological and developmental processes and play a role in cell proliferation, cell motility, and receptor trafficking. In mammals, Sproutys regulate different developmental branching processes, like lung development (79), angiogenesis (35, 80), and placenta morphogenesis (81). Moreover, they play a role during human follicle maturation (82) and myogenic differentiation (83). Surprisingly, during chick bone development (24), a contrary phenomenon was observed compared with Spred-2-null mice (56). Here, the overexpression of Sprouty (24) and not the loss of Spred-2 (56) led to a hypochondroplasia-like phenotype. Therefore, Spred and Sprouty may have different regulatory functions during this developmental process.

Phosphorylation of Sproutys is not only observed but also necessary for physiological functions (27, 52, 55, 84–86). The phosphorylation of the N-terminal Tyr-55 in Sprouty2 is necessary for the interaction with and acts as binding site for the SH2 domains of c-Cbl (27) and Grb2 (52, 76, 86). Besides this, the phosphorylation of C-terminal tyrosine residues seems to be important for the specific Sprouty inhibition (87). An additional regulatory mechanism of Sproutys is the recently discovered mitogen-activated protein kinase-interacting kinase 1 (Mnk1)-mediated serine phosphorylation, in which serine phosphorylation stabilizes Sprouty2 (88). Furthermore, Sprouty activity is controlled by SHP-2 tyrosine phosphatases, which dephosphorylate the critical tyrosine of Sproutys (89).

Knock-out mouse models begin now to bring some light into Sprouty in vivo functions. Sprouty1–/– mice displayed multiple ureteric and kidney failures due to increased sensitivity of the Wolffian duct to glial cell line-derived neurotrophic factor (GDNF)/RET signaling (90). Astonishingly, Sprouty2–/– mice did not show renal defects but suffer from enteric nerve hyperplasia and hypergangliosis resulting in esophageal achalasia, dilated esophagus, intestinal pseudo-obstruction, and abnormal physiology of the digestive motility system (91). Sprouty1–/– and Sprouty2–/– mice displayed both premature death and had, like the Spred-2–/– mice, a reduced body size (91). Another study confirmed these abnormalities, whereas these mice also had severe hearing loss with an abnormal Corti organ and abnormal cochlear hair cell morphology (92).


   Summary and Future Perspectives
TOP
 ABSTRACT
 Family Members, Structure, and...
 Domains: Structure and Functions
 Signaling Pathways and...
 Summary and Future Perspectives
REFERENCES
 
Here, we gave an overview of the three closely related protein families VASP, Spred, and Sprouty. Compared with VASP, Spred and Sprouty share a more similar overall domain organization, and comparison of both SPR domains revealed some overlapping functions. Thus, nature may use a restricted modular domain system in similar protein families as building blocks for the fine tuning of a variety of developmental processes, but there are still a lot of important unanswered questions on which future work should be focused.

Ligands and the Role of the Spred EVH1 Domain?—So far, no ligands were discovered for the Spred-EVH1 domain, but the three-dimensional structure of the Spred-EVH1 domain suggests a binding mode differing from all known EVH1 domains.

Role of the Spred KBD?—As Spred-3 with a non-functional KBD and deletion mutants of Spred-1 in this region are still functional in ERK suppression, the specific modular role of this domain is still unclear. An interesting point to follow might be the potential involvement in c-Kit-mediated signaling (93), which was shown to be altered in malignant growth, and c-Kit is also regarded as a marker for interstitial pace-making Cajal cells in the digestive system and in the urogenital tract (94, 95).

Functions of Spred and Sprouty in the Ras/MAPK Pathway?—Spreds and Sproutys are inhibitors of the Ras/MAPK pathway, and both groups seem to be able to inhibit at different levels, surely dependent on the intracellular context. Detailed clarification of the spatial distribution, and functional interactions will be very important for a deeper understanding. Sproutys can also positively regulate RTK signaling, but such mechanisms are not known for Spreds. Therefore, Spreds and Sproutys might provide complementary and contrary modes for RTK signaling, and especially examination of the different functions during bone development seems very interesting.

Regulatory Mechanisms?—For VASP and Sprouty proteins, the phosphorylation state regulated by different kinases and phosphatases at distinct phosphorylation sites was shown to be functionally very important. However, whether the known sites and kinases are already complete and if phosphorylation plays an important role for Spred regulation as well are important future tasks to clarify. Furthermore, hetero-/homo-oligomerization could be an additional regulatory mechanism in Spreds.

In Vivo Roles of VASP, Spred, and Sprouty?—Recently, knock-out mouse models of all three protein families started to shed some light into their physiological functions. However, overlapping expression of highly related family members hamper the studies. Therefore, the combination of conventional and conditional knock-out mouse models to circumvent embryonic lethality of double or even triple knock-outs will enlighten the physiological functions of these protein families.

Considering all data, there is no general structural or functional model fitting all three protein families. A principle role of the VASP-EVH1 domain is to recruit an actin filament regulator to cellular sites where a spatial and temporal regulation of actin polymerization is required. Therefore, we postulate that the Spred-EVH1 domain could be required to recruit an important inhibitor of the Ras/ERK/MAPK pathway to cellular sites where this inhibition will be essential. The identification of Spred-EVH1 ligands will begin to unravel this important question.


   FOOTNOTES
 
* This minireview will be reprinted in the 2006 Minireview Compendium, which will be available in January, 2007. This work was supported by the Ministerium für Wissenschaft, Forschung und Kunst, Baden-Württemberg, Germany ("Stipendium der Universität Ulm" (to K. B.) and by the DFG. Back

1 To whom correspondence should be addressed. Tel.: 49-731-50-23281; Fax: 49-731-50-23277; E-mail: karin.bundschu{at}gmx.de.

2 The abbreviations used are: Spred, Sprouty-related protein with an EVH1 domain; VASP, vasodilator-stimulated phosphoprotein; EVH1, Ena/vasodilator-stimulated phosphoprotein homology1; KBD, c-Kit binding domain; SPR, Sprouty-related domain; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; RTK, receptor tyrosine kinase. Back

3 C. Blume, personal communication. Back



   REFERENCES
TOP
 ABSTRACT
 Family Members, Structure, and...
 Domains: Structure and Functions
 Signaling Pathways and...
 Summary and Future Perspectives
 REFERENCES
 

  1. DeMille, M. M., Kimmel, B. E., and Rubin, G. M. (1996) Gene (Amst.) 183, 103–108[CrossRef][Medline] [Order article via Infotrieve]
  2. Kato, R., Nonami, A., Taketomi, T., Wakioka, T., Kuroiwa, A., Matsuda, Y., and Yoshimura, A. (2003) Biochem. Biophys. Res. Commun. 302, 767–772[CrossRef][Medline] [Order article via Infotrieve]
  3. Sivak, J. M., Petersen, L. F., and Amaya, E. (2005) Dev. Cell 8, 689–701[CrossRef][Medline] [Order article via Infotrieve]
  4. King, J. A., Corcoran, N. M., D'Abaco, G. M., Straffon, A. F., Smith, C. T., Poon, C. L., Buchert, M., I, S., Hall, N. E., Lock, P., and Hovens, C. M. (2006) J. Hepatol. 44, 758–767[CrossRef][Medline] [Order article via Infotrieve]
  5. Wakioka, T., Sasaki, A., Kato, R., Shouda, T., Matsumoto, A., Miyoshi, K., Tsuneoka, M., Komiya, S., Baron, R., and Yoshimura, A. (2001) Nature 412, 647–651[CrossRef][Medline] [Order article via Infotrieve]
  6. Sasaki, A., Taketomi, T., Kato, R., Saeki, K., Nonami, A., Sasaki, M., Kuriyama, M., Saito, N., Shibuya, M., and Yoshimura, A. (2003) Nat. Cell Biol. 5, 427–432[CrossRef][Medline] [Order article via Infotrieve]
  7. Miyoshi, K., Wakioka, T., Nishinakamura, H., Kamio, M., Yang, L., Inoue, M., Hasegawa, M., Yonemitsu, Y., Komiya, S., and Yoshimura, A. (2004) Oncogene 23, 5567–5576[CrossRef][Medline] [Order article via Infotrieve]
  8. Nonami, A., Taketomi, T., Kimura, A., Saeki, K., Takaki, H., Sanada, T., Taniguchi, K., Harada, M., Kato, R., and Yoshimura, A. (2005) Genes Cells 10, 887–895[Abstract/Free Full Text]
  9. Engelhardt, C. M., Bundschu, K., Messerschmitt, M., Renne, T., Walter, U., Reinhard, M., and Schuh, K. (2004) Histochem. Cell Biol. 122, 527–538[CrossRef][Medline] [Order article via Infotrieve]
  10. Bundschu, K., Gattenlohner, S., Knobeloch, K. P., Walter, U., and Schuh, K. (2006) Gene Expr. Patterns 6, 247–255[CrossRef][Medline] [Order article via Infotrieve]
  11. Halbrugge, M., and Walter, U. (1989) Eur. J. Biochem. 185, 41–50[Medline] [Order article via Infotrieve]
  12. Haffner, C., Jarchau, T., Reinhard, M., Hoppe, J., Lohmann, S. M., and Walter, U. (1995) EMBO J. 14, 19–27[Medline] [Order article via Infotrieve]
  13. Gertler, F. B., Niebuhr, K., Reinhard, M., Wehland, J., and Soriano, P. (1996) Cell 87, 227–239[CrossRef][Medline] [Order article via Infotrieve]
  14. Lanier, L. M., and Gertler, F. B. (2000) Curr. Opin. Neurobiol. 10, 80–87[CrossRef][Medline] [Order article via Infotrieve]
  15. Reinhard, M., Jarchau, T., and Walter, U. (2001) Trends Biochem. Sci. 26, 243–249[CrossRef][Medline] [Order article via Infotrieve]
  16. Bachmann, C., Fischer, L., Walter, U., and Reinhard, M. (1999) J. Biol. Chem. 274, 23549–23557[Abstract/Free Full Text]
  17. Huttelmaier, S., Harbeck, B., Steffens, O., Messerschmidt, T., Illenberger, S., and Jockusch, B. M. (1999) FEBS Lett. 451, 68–74[CrossRef][Medline] [Order article via Infotrieve]
  18. Harbeck, B., Huttelmaier, S., Schluter, K., Jockusch, B. M., and Illenberger, S. (2000) J. Biol. Chem. 275, 30817–30825[Abstract/Free Full Text]
  19. Reinhard, M., Zumbrunn, J., Jaquemar, D., Kuhn, M., Walter, U., and Trueb, B. (1999) J. Biol. Chem. 274, 13410–13418[Abstract/Free Full Text]
  20. Colavita, A., and Culotti, J. G. (1998) Dev. Biol. 194, 72–85[CrossRef][Medline] [Order article via Infotrieve]
  21. Hacohen, N., Kramer, S., Sutherland, D., Hiromi, Y., and Krasnow, M. A. (1998) Cell 92, 253–263[CrossRef][Medline] [Order article via Infotrieve]
  22. Tefft, J. D., Lee, M., Smith, S., Leinwand, M., Zhao, J., Bringas, P., Jr., Crowe, D. L., and Warburton, D. (1999) Curr. Biol. 9, 219–222[CrossRef][Medline] [Order article via Infotrieve]
  23. de Maximy, A. A., Nakatake, Y., Moncada, S., Itoh, N., Thiery, J. P., and Bellusci, S. (1999) Mech. Dev. 81, 213–216[CrossRef][Medline] [Order article via Infotrieve]
  24. Minowada, G., Jarvis, L. A., Chi, C. L., Neubuser, A., Sun, X., Hacohen, N., Krasnow, M. A., and Martin, G. R. (1999) Development 126, 4465–4475[Abstract]
  25. Chambers, D., and Mason, I. (2000) Mech. Dev. 91, 361–364[CrossRef][Medline] [Order article via Infotrieve]
  26. Guy, G. R., Wong, E. S., Yusoff, P., Chandramouli, S., Lo, T. L., Lim, J., and Fong, C. W. (2003) J. Cell Sci. 116, 3061–3068[Abstract/Free Full Text]
  27. Rubin, C., Litvak, V., Medvedovsky, H., Zwang, Y., Lev, S., and Yarden, Y. (2003) Curr. Biol. 13, 297–307[CrossRef][Medline] [Order article via Infotrieve]
  28. Nutt, S. L., Dingwell, K. S., Holt, C. E., and Amaya, E. (2001) Genes Dev. 15, 1152–1166[Abstract/Free Full Text]
  29. Dikic, I., and Giordano, S. (2003) Curr. Opin. Cell Biol. 15, 128–135[CrossRef][Medline] [Order article via Infotrieve]
  30. Kim, H. J., and Bar-Sagi, D. (2004) Nat. Rev. Mol. Cell. Biol. 5, 441–450[CrossRef][Medline] [Order article via Infotrieve]
  31. Impagnatiello, M. A., Weitzer, S., Gannon, G., Compagni, A., Cotten, M., and Christofori, G. (2001) J. Cell Biol. 152, 1087–1098[Abstract/Free Full Text]
  32. Tsumura, Y., Toshima, J., Leeksma, O. C., Ohashi, K., and Mizuno, K. (2005) Biochem. J. 387, 627–637[CrossRef][Medline] [Order article via Infotrieve]
  33. Yigzaw, Y., Poppleton, H. M., Sreejayan, N., Hassid, A., and Patel, T. B. (2003) J. Biol. Chem. 278, 284–288[Abstract/Free Full Text]
  34. Leeksma, O. C., Van Achterberg, T. A., Tsumura, Y., Toshima, J., Eldering, E., Kroes, W. G., Mellink, C., Spaargaren, M., Mizuno, K., Pannekoek, H., and de Vries, C. J. (2002) Eur. J. Biochem. 269, 2546–2556[Medline] [Order article via Infotrieve]
  35. Lee, S. H., Schloss, D. J., Jarvis, L., Krasnow, M. A., and Swain, J. L. (2001) J. Biol. Chem. 276, 4128–4133[Abstract/Free Full Text]
  36. Lim, J., Wong, E. S., Ong, S. H., Yusoff, P., Low, B. C., and Guy, G. R. (2000) J. Biol. Chem. 275, 32837–32845[Abstract/Free Full Text]
  37. Lim, J., Yusoff, P., Wong, E. S., Chandramouli, S., Lao, D. H., Fong, C. W., and Guy, G. R. (2002) Mol. Cell. Biol. 22, 7953–7966[Abstract/Free Full Text]
  38. Reinhard, M., Jouvenal, K., Tripier, D., and Walter, U. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 7956–7960[Abstract/Free Full Text]
  39. Reinhard, M., Rudiger, M., Jockusch, B. M., and Walter, U. (1996) FEBS Lett. 399, 103–107[CrossRef][Medline] [Order article via Infotrieve]
  40. Niebuhr, K., Ebel, F., Frank, R., Reinhard, M., Domann, E., Carl, U. D., Walter, U., Gertler, F. B., Wehland, J., and Chakraborty, T. (1997) EMBO J. 16, 5433–5444[CrossRef][Medline] [Order article via Infotrieve]
  41. Ball, L. J., Jarchau, T., Oschkinat, H., and Walter, U. (2002) FEBS Lett. 513, 45–52[CrossRef][Medline] [Order article via Infotrieve]
  42. Renfranz, P. J., and Beckerle, M. C. (2002) Curr. Opin. Cell Biol. 14, 88–103[CrossRef][Medline] [Order article via Infotrieve]
  43. Klostermann, A., Lutz, B., Gertler, F., and Behl, C. (2000) J. Biol. Chem. 275, 39647–39653[Abstract/Free Full Text]
  44. Bashaw, G. J., Kidd, T., Murray, D., Pawson, T., and Goodman, C. S. (2000) Cell 101, 703–715[CrossRef][Medline] [Order article via Infotrieve]
  45. Petit, M. M., Fradelizi, J., Golsteyn, R. M., Ayoubi, T. A., Menichi, B., Louvard, D., Van de Ven, W. J., and Friederich, E. (2000) Mol. Biol. Cell 11, 117–129[Abstract/Free Full Text]
  46. Krause, M., Sechi, A. S., Konradt, M., Monner, D., Gertler, F. B., and Wehland, J. (2000) J. Cell Biol. 149, 181–194[Abstract/Free Full Text]
  47. King, J. A., Straffon, A. F., D'Abaco, G. M., Poon, C. L., I, S. T., Smith, C. M., Buchert, M., Corcoran, N. M., Hall, N. E., Callus, B. A., Sarcevic, B., Martin, D., Lock, P., and Hovens, C. M. (2005) Biochem. J. 388, 445–454[CrossRef][Medline] [Order article via Infotrieve]
  48. Zimmermann, J., Jarchau, T., Walter, U., Oschkinat, H., and Ball, L. J. (2004) J. Biomol. NMR 29, 435–436[CrossRef][Medline] [Order article via Infotrieve]
  49. Harmer, N. J., Sivak, J. M., Amaya, E., and Blundell, T. L. (2005) FEBS Lett. 579, 1161–1166[CrossRef][Medline] [Order article via Infotrieve]
  50. Nonami, A., Kato, R., Taniguchi, K., Yoshiga, D., Taketomi, T., Fukuyama, S., Harada, M., Sasaki, A., and Yoshimura, A. (2004) J. Biol. Chem. 279, 52543–52551[Abstract/Free Full Text]
  51. Nobuhisa, I., Kato, R., Inoue, H., Takizawa, M., Okita, K., Yoshimura, A., and Taga, T. (2004) J. Exp. Med. 199, 737–742[Abstract/Free Full Text]
  52. Hanafusa, H., Torii, S., Yasunaga, T., and Nishida, E. (2002) Nat. Cell Biol. 4, 850–858[CrossRef][Medline] [Order article via Infotrieve]
  53. Yigzaw, Y., Cartin, L., Pierre, S., Scholich, K., and Patel, T. B. (2001) J. Biol. Chem. 276, 22742–22747[Abstract/Free Full Text]
  54. Ozaki, K., Miyazaki, S., Tanimura, S., and Kohno, M. (2005) J. Cell Sci. 118, 5861–5871[Abstract/Free Full Text]
  55. Sasaki, A., Taketomi, T., Wakioka, T., Kato, R., and Yoshimura, A. (2001) J. Biol. Chem. 276, 36804–36808[Abstract/Free Full Text]
  56. Bundschu, K., Knobeloch, K. P., Ullrich, M., Schinke, T., Amling, M., Engelhardt, C. M., Renne, T., Walter, U., and Schuh, K. (2005) J. Biol. Chem. 280, 28572–28580[Abstract/Free Full Text]
  57. Inoue, H., Kato, R., Fukuyama, S., Nonami, A., Taniguchi, K., Matsumoto, K., Nakano, T., Tsuda, M., Matsumura, M., Kubo, M., Ishikawa, F., Moon, B. G., Takatsu, K., Nakanishi, Y., and Yoshimura, A. (2005) J. Exp. Med. 201, 73–82[Abstract/Free Full Text]
  58. Barzik, M., Kotova, T. I., Higgs, H. N., Hazelwood, L., Hanein, D., Gertler, F. B., and Schafer, D. A. (2005) J. Biol. Chem. 280, 28653–28662[Abstract/Free Full Text]
  59. Schirenbeck, A., Arasada, R., Bretschneider, T., Stradal, T. E., Schleicher, M., and Faix, J. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 7694–7699[Abstract/Free Full Text]
  60. Ahern-Djamali, S. M., Bachmann, C., Hua, P., Reddy, S. K., Kastenmeier, A. S., Walter, U., and Hoffmann, F. M. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 4977–4982[Abstract/Free Full Text]
  61. Drees, B., Friederich, E., Fradelizi, J., Louvard, D., Beckerle, M. C., and Golsteyn, R. M. (2000) J. Biol. Chem. 275, 22503–22511[Abstract/Free Full Text]
  62. Butt, E., Abel, K., Krieger, M., Palm, D., Hoppe, V., Hoppe, J., and Walter, U. (1994) J. Biol. Chem. 269, 14509–14517[Abstract/Free Full Text]
  63. Zhuang, S., Nguyen, G. T., Chen, Y., Gudi, T., Eigenthaler, M., Jarchau, T., Walter, U., Boss, G. R., and Pilz, R. B. (2004) J. Biol. Chem. 279, 10397–10407[Abstract/Free Full Text]
  64. Chitaley, K., Chen, L., Galler, A., Walter, U., Daum, G., and Clowes, A. W. (2004) FEBS Lett. 556, 211–215[CrossRef][Medline] [Order article via Infotrieve]
  65. Abel, K., Mieskes, G., and Walter, U. (1995) FEBS Lett. 370, 184–188[CrossRef][Medline] [Order article via Infotrieve]
  66. Reinhard, M., Giehl, K., Abel, K., Haffner, C., Jarchau, T., Hoppe, V., Jockusch, B. M., and Walter, U. (1995) EMBO J. 14, 1583–1589[Medline] [Order article via Infotrieve]
  67. Kuhnel, K., Jarchau, T., Wolf, E., Schlichting, I., Walter, U., Wittinghofer, A., and Strelkov, S. V. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 17027–17032[Abstract/Free Full Text]
  68. Aszodi, A., Pfeifer, A., Ahmad, M., Glauner, M., Zhou, X. H., Ny, L., Andersson, K. E., Kehrel, B., Offermanns, S., and Fassler, R. (1999) EMBO J. 18, 37–48[CrossRef][Medline] [Order article via Infotrieve]
  69. Hauser, W., Knobeloch, K. P., Eigenthaler, M., Gambaryan, S., Krenn, V., Geiger, J., Glazova, M., Rohde, E., Horak, I., Walter, U., and Zimmer, M. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 8120–8125[Abstract/Free Full Text]
  70. Lanier, L. M., Gates, M. A., Witke, W., Menzies, A. S., Wehman, A. M., Macklis, J. D., Kwiatkowski, D., Soriano, P., and Gertler, F. B. (1999) Neuron 22, 313–325[CrossRef][Medline] [Order article via Infotrieve]
  71. Massberg, S., Gruner, S., Konrad, I., Garcia Arguinzonis, M. I., Eigenthaler, M., Hemler, K., Kersting, J., Schulz, C., Muller, I., Besta, F., Nieswandt, B., Heinzmann, U., Walter, U., and Gawaz, M. (2004) Blood 103, 136–142[Abstract/Free Full Text]
  72. Menzies, A. S., Aszodi, A., Williams, S. E., Pfeifer, A., Wehman, A. M., Goh, K. L., Mason, C. A., Fassler, R., and Gertler, F. B. (2004) J. Neurosci. 24, 8029–8038[Abstract/Free Full Text]
  73. Kramer, S., Okabe, M., Hacohen, N., Krasnow, M. A., and Hiromi, Y. (1999) Development 126, 2515–2525[Abstract]
  74. Reich, A., Sapir, A., and Shilo, B. (1999) Development 126, 4139–4147[Abstract]
  75. Egan, J. E., Hall, A. B., Yatsula, B. A., and Bar-Sagi, D. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 6041–6046[Abstract/Free Full Text]
  76. Wong, E. S., Lim, J., Low, B. C., Chen, Q., and Guy, G. R. (2001) J. Biol. Chem. 276, 5866–5875[Abstract/Free Full Text]
  77. Cabrita, M. A., and Christofori, G. (2003) Thromb. Haemostasis 90, 586–590[Medline] [Order article via Infotrieve]
  78. Christofori, G. (2003) Nat. Cell Biol. 5, 377–379[CrossRef][Medline] [Order article via Infotrieve]
  79. Mailleux, A. A., Tefft, D., Ndiaye, D., Itoh, N., Thiery, J. P., Warburton, D., and Bellusci, S. (2001) Mech. Dev. 102, 81–94[CrossRef][Medline] [Order article via Infotrieve]
  80. Zhang, C., Chaturvedi, D., Jaggar, L., Magnuson, D., Lee, J. M., and Patel, T. B. (2005) Arterioscler. Thromb. Vasc. Biol. 25, 533–538[Abstract/Free Full Text]
  81. Anteby, E. Y., Natanson-Yaron, S., Greenfield, C., Goldman-Wohl, D., Haimov-Kochman, R., Holzer, H., and Yagel, S. (2005) Placenta 26, 476–483[CrossRef][Medline] [Order article via Infotrieve]
  82. Haimov-Kochman, R., Ravhon, A., Prus, D., Greenfield, C., Finci-Yeheskel, Z., D, S. G.-W., Natanson-Yaron, S., Reich, R., Yagel, S., and Hurwitz, A. (2005) Mol. Hum. Reprod. 11, 537–542[Abstract/Free Full Text]
  83. de Alvaro, C., Martinez, N., Rojas, J. M., and Lorenzo, M. (2005) Mol. Biol. Cell 16, 4454–4461[Abstract/Free Full Text]
  84. Tefft, D., Lee, M., Smith, S., Crowe, D. L., Bellusci, S., and Warburton, D. (2002) Am. J. Physiol. 283, L700–L706
  85. Mason, J. M., Morrison, D. J., Bassit, B., Dimri, M., Band, H., Licht, J. D., and Gross, I. (2004) Mol. Biol. Cell 15, 2176–2188[Abstract/Free Full Text]
  86. Fong, C. W., Leong, H. F., Wong, E. S., Lim, J., Yusoff, P., and Guy, G. R. (2003) J. Biol. Chem. 278, 33456–33464[Abstract/Free Full Text]
  87. Rubin, C., Zwang, Y., Vaisman, N., Ron, D., and Yarden, Y. (2005) J. Biol. Chem. 280, 9735–9744[Abstract/Free Full Text]
  88. DaSilva, J., Xu, L., Kim, H. J., Miller, W. T., and Bar-Sagi, D. (2006) Mol. Cell. Biol. 26, 1898–1907[Abstract/Free Full Text]
  89. Jarvis, L. A., Toering, S. J., Simon, M. A., Krasnow, M. A., and Smith-Bolton, R. K. (2006) Development 133, 1133–1142[Abstract/Free Full Text]
  90. Basson, M. A., Akbulut, S., Watson-Johnson, J., Simon, R., Carroll, T. J., Shakya, R., Gross, I., Martin, G. R., Lufkin, T., McMahon, A. P., Wilson, P. D., Costantini, F. D., Mason, I. J., and Licht, J. D. (2005) Dev. Cell 8, 229–239[CrossRef][Medline] [Order article via Infotrieve]
  91. Taketomi, T., Yoshiga, D., Taniguchi, K., Kobayashi, T., Nonami, A., Kato, R., Sasaki, M., Sasaki, A., Ishibashi, H., Moriyama, M., Nakamura, K., Nishimura, J., and Yoshimura, A. (2005) Nat. Neurosci. 8, 855–857[Medline] [Order article via Infotrieve]
  92. Shim, K., Minowada, G., Coling, D. E., and Martin, G. R. (2005) Dev. Cell 8, 553–564[CrossRef][Medline] [Order article via Infotrieve]
  93. Roskoski, R., Jr. (2005) Biochem. Biophys. Res. Commun. 337, 1–13[CrossRef][Medline] [Order article via Infotrieve]
  94. Hanani, M., and Freund, H. R. (2000) Acta Physiol. Scand. 170, 177–190[CrossRef][Medline] [Order article via Infotrieve]
  95. Brading, A. F., and McCloskey, K. D. (2005) Nat. Clin. Pract. Urol. 2, 546–554[Medline] [Order article via Infotrieve]

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C. Johne, D. Matenia, X.-y. Li, T. Timm, K. Balusamy, and E.-M. Mandelkow
Spred1 and TESK1--Two New Interaction Partners of the Kinase MARKK/TAO1 That Link the Microtubule and Actin Cytoskeleton
Mol. Biol. Cell, April 1, 2008; 19(4): 1391 - 1403.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. C. Kraemer and G. D. Schellenberg
SUT-1 enables tau-induced neurotoxicity in C. elegans
Hum. Mol. Genet., August 15, 2007; 16(16): 1959 - 1971.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/48/36477    most recent
R600023200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager